Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.

Detalhes bibliográficos
Autor(a) principal: CATTANI, A. M.
Data de Publicação: 2020
Outros Autores: FALAVIGNA, V. da S., SILVEIRA, C. P., BUFFON, V., MARASCHIN, F. dos S., PASQUALI, G., REVERS, L. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127859
Resumo: Key Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · Apple
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spelling Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.MdoBRRBud dormancyCytokininMdoDAM1ApplesKey Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · AppleAMANDA MALVESSI CATTANI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; VÍTOR DA SILVEIRA FALAVIGNA, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Köln, Germany; CAROLINA PEREIRA SILVEIRA, Laboratory of Plant Molecular Genetics, Embrapa Uva e Vinho, Bento Gonçalves, RS, Brazil; VANESSA BUFFON, CNPUV; FELIPE DOS SANTOS MARASCHIN, Departament of Botany, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; GIANCARLO PASQUALI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; LUIS FERNANDO REVERS, CNPUV.CATTANI, A. M.FALAVIGNA, V. da S.SILVEIRA, C. P.BUFFON, V.MARASCHIN, F. dos S.PASQUALI, G.REVERS, L. F.2020-12-10T09:03:34Z2020-12-10T09:03:34Z2020-12-082020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Cell Reports, v. 39, p. 1687-1703, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112785910.1007_s00299-020-02595-z.pdfenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-12-10T09:03:42Zoai:www.alice.cnptia.embrapa.br:doc/1127859Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-12-10T09:03:42Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
title Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
spellingShingle Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
CATTANI, A. M.
MdoBRR
Bud dormancy
Cytokinin
MdoDAM1
Apples
title_short Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
title_full Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
title_fullStr Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
title_full_unstemmed Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
title_sort Type-B cytokinin response regulators link hormonal stimuli and molecular responses during the transition from endoto ecodormancy in apple buds.
author CATTANI, A. M.
author_facet CATTANI, A. M.
FALAVIGNA, V. da S.
SILVEIRA, C. P.
BUFFON, V.
MARASCHIN, F. dos S.
PASQUALI, G.
REVERS, L. F.
author_role author
author2 FALAVIGNA, V. da S.
SILVEIRA, C. P.
BUFFON, V.
MARASCHIN, F. dos S.
PASQUALI, G.
REVERS, L. F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv AMANDA MALVESSI CATTANI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; VÍTOR DA SILVEIRA FALAVIGNA, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Köln, Germany; CAROLINA PEREIRA SILVEIRA, Laboratory of Plant Molecular Genetics, Embrapa Uva e Vinho, Bento Gonçalves, RS, Brazil; VANESSA BUFFON, CNPUV; FELIPE DOS SANTOS MARASCHIN, Departament of Botany, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; GIANCARLO PASQUALI, Graduate Program in Cell and Molecular Biology, Center for Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; LUIS FERNANDO REVERS, CNPUV.
dc.contributor.author.fl_str_mv CATTANI, A. M.
FALAVIGNA, V. da S.
SILVEIRA, C. P.
BUFFON, V.
MARASCHIN, F. dos S.
PASQUALI, G.
REVERS, L. F.
dc.subject.por.fl_str_mv MdoBRR
Bud dormancy
Cytokinin
MdoDAM1
Apples
topic MdoBRR
Bud dormancy
Cytokinin
MdoDAM1
Apples
description Key Message Cytokinin together with MdoBRR1, MdoBRR8 and MdoBRR10 genes participate in the downregulation of MdoDAM1, contributing to the transition from endo- to ecodormancy in apple buds. Abstract The fnal step of cytokinin (CK) signaling pathway culminates in the activation of type-B response regulators (BRRs), important transcriptional factors in the modulation of CK-responsive genes. In this study, we performed a genomewide analysis aiming to identify apple BRR family members and understand their involvement in bud dormancy control. The investigation identifed ten MdoBRR protein-coding genes. A higher expression of three MdoBRR (MdoBRR1, MdoBRR9 and MdoBRR10) was observed in dormant buds in comparison to other developmental stages. Interestingly, in ecodormant buds these three MdoBRR genes were upregulated in a CK-dependent manner. Transcription profles, determined during dormancy cycle under feld and artifcially controlled conditions, revealed that MdoBRR1 and MdoBRR8 played important roles in the transition from endo- to ecodormancy, probably mediated by endogenous CK stimuli. The expression of MdoBRR7, MdoBRR9, and MdoBRR10 was induced in ecodormant buds exposed to warm temperatures, indicating a putative role in growth resumption after chilling requirement fulfllment. Contrasting expression patternsin vivo between MdoBRRs and MdoDAM1, an essential dormancy establishment regulator, were observed during dormancy cycle and in CK-treated buds. Thereafter, in vivo transactivation assays showed that CK stimuli combined with transient overexpression of MdoBRR1, MdoBRR8, and MdoBRR10 resulted in downregulation of the reporter gene gusA driven by the MdoDAM1 promoter. These pieces of evidences point to the integration of CK-triggered responses through MdoBRRs that are able to downregulate MdoDAM1, contributing to dormancy release in apple. Keywords MdoBRR · Cytokinin · Bud dormancy · MdoDAM1 · Apple
publishDate 2020
dc.date.none.fl_str_mv 2020-12-10T09:03:34Z
2020-12-10T09:03:34Z
2020-12-08
2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plant Cell Reports, v. 39, p. 1687-1703, 2020.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127859
10.1007_s00299-020-02595-z.pdf
identifier_str_mv Plant Cell Reports, v. 39, p. 1687-1703, 2020.
10.1007_s00299-020-02595-z.pdf
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127859
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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