Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Agropecuária Catarinense (Online) |
Texto Completo: | https://publicacoes.epagri.sc.gov.br/rac/article/view/718 |
Resumo: | Banana plants were regenerated using tissue culture of top buds from Cavendish plants, cultivar Grande Naine, maintained under ten successive cultivations in different culture media. The genomic DNA was extractedfrom the original matrix plant rhizoms, also from the dwarf mutant ones, variegated plants and from the plants originated from the five and ten successive in vitro cultivations. The Random Amplified Polymorphic DNA (RAPD) analysis was carried out using 20 primers, having two of them (10%) shown polymorphism among the dwarf and variegated plants in relation to the normal plants. The OPJ-04 primer has amplified two fragments of approximately 1.5 and 2.0kb present in the normal plants, but absent in the dwarf ones. The OPH-09 primer, however, presentedan amplification of approximately 1.7kb fragment, present in both dwarf and in the variegated plants, but absent in the normal ones. This study shows that using the RAPD molecular marker makes it is possible to detect induced somaclonal variation through micropropagation in banana plants. |
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Agropecuária Catarinense (Online) |
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Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD markerAnálise da variação somaclonal em mudas micropropagadas de Musa acuminata cultivar Grande Naine por meio de marcadores RAPDbanana plantstissue culturemutationmolecular markerbananacultura de tecidosmutaçãomarcador molecularBanana plants were regenerated using tissue culture of top buds from Cavendish plants, cultivar Grande Naine, maintained under ten successive cultivations in different culture media. The genomic DNA was extractedfrom the original matrix plant rhizoms, also from the dwarf mutant ones, variegated plants and from the plants originated from the five and ten successive in vitro cultivations. The Random Amplified Polymorphic DNA (RAPD) analysis was carried out using 20 primers, having two of them (10%) shown polymorphism among the dwarf and variegated plants in relation to the normal plants. The OPJ-04 primer has amplified two fragments of approximately 1.5 and 2.0kb present in the normal plants, but absent in the dwarf ones. The OPH-09 primer, however, presentedan amplification of approximately 1.7kb fragment, present in both dwarf and in the variegated plants, but absent in the normal ones. This study shows that using the RAPD molecular marker makes it is possible to detect induced somaclonal variation through micropropagation in banana plants. Plantas de banana foram regeneradas através da cultura de tecidos a partir de gemas apicais de bananeira Cavendish, cultivar Grande Naine, e mantidas por dez subcultivos em diferentes meios de cultura. O DNA genômico foi extraído a partir de rizomas das plantas matrizes originais, das plantas mutantes anãs e variegadas oriundas do cultivo in vitro e das plantas provindas de cinco e dez subcultivos. A análise, Random Amplified Polymorphic DNA (RAPD) foi realizada utilizando-se 20 “primers” (iniciadores – sequência conhecida de nucleotídeos3), dois dos quais (10%) revelaram polimorfismo entre as plantas anãs e variegadas em relação às plantas normais. Com a utilização do “primer” OPJ-04, foram amplificados, nas plantas normais, dois fragmentos de aproximadamente 1,5 e 2,0kb (quilobase, mil pares de bases) os quais estavam ausentes nas plantas anãs. Já o “primer” OPH-09 apresentou aamplificação de uma banda de aproximadamente 1,7kb presente tanto nas plantas anãs quanto nas variegadas, mas ausente nas plantas normais. Neste estudo, mostrou-se que através do uso de marcador molecular RAPD é possível detectar variação somaclonal surgida durante o processo de micropropagação nos mutantes. Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri2020-04-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://publicacoes.epagri.sc.gov.br/rac/article/view/718Agropecuária Catarinense Journal; Vol. 23 No. 1 (2010); 76-80Agropecuária Catarinense; v. 23 n. 1 (2010); 76-802525-60760103-0779reponame:Agropecuária Catarinense (Online)instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)instacron:EPAGRIporhttps://publicacoes.epagri.sc.gov.br/rac/article/view/718/618Copyright (c) 2020 Revista Agropecuária Catarinenseinfo:eu-repo/semantics/openAccessZaffari, Gilmar RobertoKerbauy, Gilberto Barbante2021-06-23T14:37:38Zoai:ojs.publicacoes.epagri.sc.gov.br:article/718Revistahttps://publicacoes.epagri.sc.gov.br/RAC/indexPUBhttps://publicacoes.epagri.sc.gov.br/index.php/RAC/oaieditoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br2525-60760103-0779opendoar:2021-06-23T14:37:38Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)false |
dc.title.none.fl_str_mv |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker Análise da variação somaclonal em mudas micropropagadas de Musa acuminata cultivar Grande Naine por meio de marcadores RAPD |
title |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker |
spellingShingle |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker Zaffari, Gilmar Roberto banana plants tissue culture mutation molecular marker banana cultura de tecidos mutação marcador molecular |
title_short |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker |
title_full |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker |
title_fullStr |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker |
title_full_unstemmed |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker |
title_sort |
Somaclonal variation analysis in micropropagated plants of Musa acuminata cultivar Grande Naine using RAPD marker |
author |
Zaffari, Gilmar Roberto |
author_facet |
Zaffari, Gilmar Roberto Kerbauy, Gilberto Barbante |
author_role |
author |
author2 |
Kerbauy, Gilberto Barbante |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Zaffari, Gilmar Roberto Kerbauy, Gilberto Barbante |
dc.subject.por.fl_str_mv |
banana plants tissue culture mutation molecular marker banana cultura de tecidos mutação marcador molecular |
topic |
banana plants tissue culture mutation molecular marker banana cultura de tecidos mutação marcador molecular |
description |
Banana plants were regenerated using tissue culture of top buds from Cavendish plants, cultivar Grande Naine, maintained under ten successive cultivations in different culture media. The genomic DNA was extractedfrom the original matrix plant rhizoms, also from the dwarf mutant ones, variegated plants and from the plants originated from the five and ten successive in vitro cultivations. The Random Amplified Polymorphic DNA (RAPD) analysis was carried out using 20 primers, having two of them (10%) shown polymorphism among the dwarf and variegated plants in relation to the normal plants. The OPJ-04 primer has amplified two fragments of approximately 1.5 and 2.0kb present in the normal plants, but absent in the dwarf ones. The OPH-09 primer, however, presentedan amplification of approximately 1.7kb fragment, present in both dwarf and in the variegated plants, but absent in the normal ones. This study shows that using the RAPD molecular marker makes it is possible to detect induced somaclonal variation through micropropagation in banana plants. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://publicacoes.epagri.sc.gov.br/rac/article/view/718 |
url |
https://publicacoes.epagri.sc.gov.br/rac/article/view/718 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://publicacoes.epagri.sc.gov.br/rac/article/view/718/618 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista Agropecuária Catarinense info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista Agropecuária Catarinense |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri |
publisher.none.fl_str_mv |
Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri |
dc.source.none.fl_str_mv |
Agropecuária Catarinense Journal; Vol. 23 No. 1 (2010); 76-80 Agropecuária Catarinense; v. 23 n. 1 (2010); 76-80 2525-6076 0103-0779 reponame:Agropecuária Catarinense (Online) instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri) instacron:EPAGRI |
instname_str |
Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri) |
instacron_str |
EPAGRI |
institution |
EPAGRI |
reponame_str |
Agropecuária Catarinense (Online) |
collection |
Agropecuária Catarinense (Online) |
repository.name.fl_str_mv |
Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri) |
repository.mail.fl_str_mv |
editoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br |
_version_ |
1754917259795496960 |