Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene

Detalhes bibliográficos
Autor(a) principal: Corrêa Puttkammer, Catarina
Data de Publicação: 2021
Outros Autores: Zappelini, Julia, Henrique Ferrero Klabunde, Gustavo, Pedro Guerra, Miguel
Tipo de documento: Artigo
Idioma: por
Título da fonte: Agropecuária Catarinense (Online)
Texto Completo: https://publicacoes.epagri.sc.gov.br/rac/article/view/1115
Resumo: The cultivated strawberry, Fragaria x ananassa Duch. (Rosaceae) is a hybrid from the crossing of American species Fragaria chiloensis and Fragaria virginiana, belonging to the Rosaceae family. Strawberry plants have vegetative reproduction and, therefore, it is common the propagation of viruses and other diseases. One of the four most important viruses in strawberry is the Strawberry vein banding virus (SVBV), which is transmitted by aphids in a semi-persistent manner. Molecular techniques are recommended to improve genome knowledge and classification of the SVBV isolates. The coat protein (CP) is a primary determinant of aphid transmissibility and specificity, critically important to infection establishment. In the present work, we sequenced the SVBV coat protein partial gene of a German strain, inoculated in strawberry plants maintained in green house in Brazil for ten years. It was performed phylogenetic analysis comparing to SVBV coat protein sequences from the GenBank database. Aiming to elucidate evolutionary relationships in this species, phylogenetic analysis showed that our SVBV sequence is closely related to USA and Egypt virus strains. These results will contribute to a better elucidation of evolutionary mechanisms and the SVBV-strawberry pathosystem in question.
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spelling Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein geneDetecção molecular e análise filogenética da sequência parcial do gene da proteína do capsídeo do vírus da faixa das nervuras do morangueiroCaulimovirusPhylogenyNeighbor-joiningSVBVvirus detectionCaulimovirusfilogeniaNeighbor-joiningdetecção de virosesSVBVThe cultivated strawberry, Fragaria x ananassa Duch. (Rosaceae) is a hybrid from the crossing of American species Fragaria chiloensis and Fragaria virginiana, belonging to the Rosaceae family. Strawberry plants have vegetative reproduction and, therefore, it is common the propagation of viruses and other diseases. One of the four most important viruses in strawberry is the Strawberry vein banding virus (SVBV), which is transmitted by aphids in a semi-persistent manner. Molecular techniques are recommended to improve genome knowledge and classification of the SVBV isolates. The coat protein (CP) is a primary determinant of aphid transmissibility and specificity, critically important to infection establishment. In the present work, we sequenced the SVBV coat protein partial gene of a German strain, inoculated in strawberry plants maintained in green house in Brazil for ten years. It was performed phylogenetic analysis comparing to SVBV coat protein sequences from the GenBank database. Aiming to elucidate evolutionary relationships in this species, phylogenetic analysis showed that our SVBV sequence is closely related to USA and Egypt virus strains. These results will contribute to a better elucidation of evolutionary mechanisms and the SVBV-strawberry pathosystem in question.O morango cultivado (Fragaria x ananassa Duch. (Rosaceae) é um híbrido originado pelo cruzamento das espécies americanas Fragaria chiloensis e Fragaria virginiana e pertence à família Rosaceae. O morangueiro possui reprodução vegetativa e, por isso, é comum o acúmulo de viroses e outras doenças de difícil controle. Uma das quatro viroses mais importantes na cultura do morango é causada pelo vírus da faixa das nervuras (Strawberry vein banding virus – SVBV), que é transmitido no campo por afídeos, de maneira semipersistente. Para melhorar o conhecimento genômico, são recomendadas técnicas moleculares e a classificação de isolados de SVBV. Um dos determinantes primários da transmissibilidade e especificidade por afídeos é a proteína do capsídeo, que possui importância crítica para o estabelecimento da infecção. Neste trabalho, foi sequenciada parcialmente a proteína do capsídeo do gene do SVBV de um isolado alemão, inoculado em morangueiros e mantido em casa de vegetação no Brasil, por mais de dez anos. Foi realizada a análise filogenética comparando as sequências contidas no GenBank com o objetivo de elucidar as relações evolutivas nesta espécie. As análises filogenéticas mostraram que a sequência do isolado está mais próxima dos isolados dos EUA e Egito. Estes resultados contribuem para a melhor elucidação dos mecanismos de evolução do vírus e do patossistema em questão.Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri2021-08-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://publicacoes.epagri.sc.gov.br/rac/article/view/111510.52945/rac.v34i2.1115Agropecuária Catarinense Journal; Vol. 34 No. 2 (2021); 37-41Agropecuária Catarinense; v. 34 n. 2 (2021); 37-412525-60760103-0779reponame:Agropecuária Catarinense (Online)instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)instacron:EPAGRIporhttps://publicacoes.epagri.sc.gov.br/rac/article/view/1115/1128Copyright (c) 2021 Catarina Corrêa Puttkammer, Julia Zappelini, Gustavo Henrique Ferrero Klabunde, Miguel Pedro Guerrahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCorrêa Puttkammer, CatarinaZappelini, JuliaHenrique Ferrero Klabunde, GustavoPedro Guerra, Miguel2021-11-08T20:17:02Zoai:ojs.publicacoes.epagri.sc.gov.br:article/1115Revistahttps://publicacoes.epagri.sc.gov.br/RAC/indexPUBhttps://publicacoes.epagri.sc.gov.br/index.php/RAC/oaieditoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br2525-60760103-0779opendoar:2021-11-08T20:17:02Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)false
dc.title.none.fl_str_mv Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
Detecção molecular e análise filogenética da sequência parcial do gene da proteína do capsídeo do vírus da faixa das nervuras do morangueiro
title Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
spellingShingle Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
Corrêa Puttkammer, Catarina
Caulimovirus
Phylogeny
Neighbor-joining
SVBV
virus detection
Caulimovirus
filogenia
Neighbor-joining
detecção de viroses
SVBV
title_short Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
title_full Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
title_fullStr Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
title_full_unstemmed Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
title_sort Molecular detection and phylogenetic analysis of partial sequence of Strawberry vein banding virus coat protein gene
author Corrêa Puttkammer, Catarina
author_facet Corrêa Puttkammer, Catarina
Zappelini, Julia
Henrique Ferrero Klabunde, Gustavo
Pedro Guerra, Miguel
author_role author
author2 Zappelini, Julia
Henrique Ferrero Klabunde, Gustavo
Pedro Guerra, Miguel
author2_role author
author
author
dc.contributor.author.fl_str_mv Corrêa Puttkammer, Catarina
Zappelini, Julia
Henrique Ferrero Klabunde, Gustavo
Pedro Guerra, Miguel
dc.subject.por.fl_str_mv Caulimovirus
Phylogeny
Neighbor-joining
SVBV
virus detection
Caulimovirus
filogenia
Neighbor-joining
detecção de viroses
SVBV
topic Caulimovirus
Phylogeny
Neighbor-joining
SVBV
virus detection
Caulimovirus
filogenia
Neighbor-joining
detecção de viroses
SVBV
description The cultivated strawberry, Fragaria x ananassa Duch. (Rosaceae) is a hybrid from the crossing of American species Fragaria chiloensis and Fragaria virginiana, belonging to the Rosaceae family. Strawberry plants have vegetative reproduction and, therefore, it is common the propagation of viruses and other diseases. One of the four most important viruses in strawberry is the Strawberry vein banding virus (SVBV), which is transmitted by aphids in a semi-persistent manner. Molecular techniques are recommended to improve genome knowledge and classification of the SVBV isolates. The coat protein (CP) is a primary determinant of aphid transmissibility and specificity, critically important to infection establishment. In the present work, we sequenced the SVBV coat protein partial gene of a German strain, inoculated in strawberry plants maintained in green house in Brazil for ten years. It was performed phylogenetic analysis comparing to SVBV coat protein sequences from the GenBank database. Aiming to elucidate evolutionary relationships in this species, phylogenetic analysis showed that our SVBV sequence is closely related to USA and Egypt virus strains. These results will contribute to a better elucidation of evolutionary mechanisms and the SVBV-strawberry pathosystem in question.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://publicacoes.epagri.sc.gov.br/rac/article/view/1115
10.52945/rac.v34i2.1115
url https://publicacoes.epagri.sc.gov.br/rac/article/view/1115
identifier_str_mv 10.52945/rac.v34i2.1115
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://publicacoes.epagri.sc.gov.br/rac/article/view/1115/1128
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri
publisher.none.fl_str_mv Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina - Epagri
dc.source.none.fl_str_mv Agropecuária Catarinense Journal; Vol. 34 No. 2 (2021); 37-41
Agropecuária Catarinense; v. 34 n. 2 (2021); 37-41
2525-6076
0103-0779
reponame:Agropecuária Catarinense (Online)
instname:Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
instacron:EPAGRI
instname_str Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
instacron_str EPAGRI
institution EPAGRI
reponame_str Agropecuária Catarinense (Online)
collection Agropecuária Catarinense (Online)
repository.name.fl_str_mv Agropecuária Catarinense (Online) - Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (Epagri)
repository.mail.fl_str_mv editoriarac@epagri.sc.gov.br || lamperuch@epagri.sc.gov.br
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