The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Laticínios Cândido Tostes |
Texto Completo: | https://www.revistadoilct.com.br/rilct/article/view/948 |
Resumo: | In this study, yoghurt fermentation time was accelerated by using the proteolytic enzyme Flavourzyme to produce sufficient peptides. Flavourzyme was added directly to the milk used for fermentation at different concentrations (0.1 g/L, 0.01 g/L, and 0.001g/L), and before heat treatment (90˚C for 15 min) where Flavourzyme was inactivated. The liberation of peptides during Flavourzyme pre-treatment was confirmed by RP-HPLC peptide profiling. It was possible to accelerate the fermentation time by 13.33% with Flavourzyme. The yoghurt made with Flavourzyme pre-treated milk did not demonstrate any significant quality changes. However, consumers were able to detect a difference between yoghurt made with Flavourzyme pre-treated milk and yoghurt made with untreated milk (control). Yoghurt made with Flavourzyme pre-treated milk scored lower in taste, texture, appearance, and overall acceptability, but was generally not rejected. Accelerated fermentation times could lead to economic advantages in the yoghurt manufacturing industry. |
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The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milkThe acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milkflavourzyme; protease; consumer satisfaction.flavourzyme; protease; satisfação do consumidor.In this study, yoghurt fermentation time was accelerated by using the proteolytic enzyme Flavourzyme to produce sufficient peptides. Flavourzyme was added directly to the milk used for fermentation at different concentrations (0.1 g/L, 0.01 g/L, and 0.001g/L), and before heat treatment (90˚C for 15 min) where Flavourzyme was inactivated. The liberation of peptides during Flavourzyme pre-treatment was confirmed by RP-HPLC peptide profiling. It was possible to accelerate the fermentation time by 13.33% with Flavourzyme. The yoghurt made with Flavourzyme pre-treated milk did not demonstrate any significant quality changes. However, consumers were able to detect a difference between yoghurt made with Flavourzyme pre-treated milk and yoghurt made with untreated milk (control). Yoghurt made with Flavourzyme pre-treated milk scored lower in taste, texture, appearance, and overall acceptability, but was generally not rejected. Accelerated fermentation times could lead to economic advantages in the yoghurt manufacturing industry.Neste estudo, o tempo de fermentação do iogurte foi acelerado usando a enzima proteolítica Flavourzyme para produzir peptídeos suficientes. Flavourzyme foi adicionado diretamente ao leite utilizado para fermentação em diferentes concentrações (0,1 g/L, 0,01 g/L e 0,001 g/L) e antes do tratamento térmico (90˚C por 15 min), onde o Flavourzyme foi inativado. A libertação de peptídeos durante o pré-tratamento com Flavourzyme foi confirmada pelo perfil peptídico de RP-HPLC. Foi possível acelerar o tempo de fermentação em 13,33% com Flavourzyme. O iogurte feito com leite pré-tratado Flavourzyme não apresentou alterações significativas na qualidade. No entanto, os consumidores conseguiram detectar uma diferença entre o iogurte feito com leite pré-tratado Flavourzyme e o iogurte feito com leite não tratado (controle). O iogurte feito com leite pré-tratado Flavourzyme obteve menor pontuação em sabor, textura, aparência e aceitabilidade geral, mas geralmente não foi rejeitado. Tempos de fermentação acelerados podem levar a vantagens econômicas na indústria de produção de iogurte.ILCTMyburgh, JacobusCalitz, Anné2024-08-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistadoilct.com.br/rilct/article/view/94810.14295/2238-6416.v77i3.948Journal of Candido Tostes Dairy Institute; v. 78, n. 3 (2023); 93-101Revista do Instituto de Laticínios Cândido Tostes; v. 78, n. 3 (2023); 93-1012238-64160100-3674reponame:Revista do Instituto de Laticínios Cândido Tostesinstname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)instacron:EPAMIGenghttps://www.revistadoilct.com.br/rilct/article/view/948/607https://www.revistadoilct.com.br/rilct/article/downloadSuppFile/948/559Direitos autorais 2024 Revista do Instituto de Laticínios Cândido Tosteshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2024-08-28T19:53:34Zoai:oai.rilct.emnuvens.com.br:article/948Revistahttp://www.revistadoilct.com.br/ONGhttps://www.revistadoilct.com.br/rilct/oai||revistadoilct@epamig.br|| revistadoilct@oi.com.br2238-64160100-3674opendoar:2024-08-28T19:53:34Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)false |
dc.title.none.fl_str_mv |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
title |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
spellingShingle |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk Myburgh, Jacobus flavourzyme; protease; consumer satisfaction. flavourzyme; protease; satisfação do consumidor. |
title_short |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
title_full |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
title_fullStr |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
title_full_unstemmed |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
title_sort |
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk |
author |
Myburgh, Jacobus |
author_facet |
Myburgh, Jacobus Calitz, Anné |
author_role |
author |
author2 |
Calitz, Anné |
author2_role |
author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Myburgh, Jacobus Calitz, Anné |
dc.subject.none.fl_str_mv |
|
dc.subject.por.fl_str_mv |
flavourzyme; protease; consumer satisfaction. flavourzyme; protease; satisfação do consumidor. |
topic |
flavourzyme; protease; consumer satisfaction. flavourzyme; protease; satisfação do consumidor. |
description |
In this study, yoghurt fermentation time was accelerated by using the proteolytic enzyme Flavourzyme to produce sufficient peptides. Flavourzyme was added directly to the milk used for fermentation at different concentrations (0.1 g/L, 0.01 g/L, and 0.001g/L), and before heat treatment (90˚C for 15 min) where Flavourzyme was inactivated. The liberation of peptides during Flavourzyme pre-treatment was confirmed by RP-HPLC peptide profiling. It was possible to accelerate the fermentation time by 13.33% with Flavourzyme. The yoghurt made with Flavourzyme pre-treated milk did not demonstrate any significant quality changes. However, consumers were able to detect a difference between yoghurt made with Flavourzyme pre-treated milk and yoghurt made with untreated milk (control). Yoghurt made with Flavourzyme pre-treated milk scored lower in taste, texture, appearance, and overall acceptability, but was generally not rejected. Accelerated fermentation times could lead to economic advantages in the yoghurt manufacturing industry. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-08-23 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistadoilct.com.br/rilct/article/view/948 10.14295/2238-6416.v77i3.948 |
url |
https://www.revistadoilct.com.br/rilct/article/view/948 |
identifier_str_mv |
10.14295/2238-6416.v77i3.948 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistadoilct.com.br/rilct/article/view/948/607 https://www.revistadoilct.com.br/rilct/article/downloadSuppFile/948/559 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2024 Revista do Instituto de Laticínios Cândido Tostes http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2024 Revista do Instituto de Laticínios Cândido Tostes http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ILCT |
publisher.none.fl_str_mv |
ILCT |
dc.source.none.fl_str_mv |
Journal of Candido Tostes Dairy Institute; v. 78, n. 3 (2023); 93-101 Revista do Instituto de Laticínios Cândido Tostes; v. 78, n. 3 (2023); 93-101 2238-6416 0100-3674 reponame:Revista do Instituto de Laticínios Cândido Tostes instname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) instacron:EPAMIG |
instname_str |
Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
instacron_str |
EPAMIG |
institution |
EPAMIG |
reponame_str |
Revista do Instituto de Laticínios Cândido Tostes |
collection |
Revista do Instituto de Laticínios Cândido Tostes |
repository.name.fl_str_mv |
Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG) |
repository.mail.fl_str_mv |
||revistadoilct@epamig.br|| revistadoilct@oi.com.br |
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1809738127743385600 |