The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk

Detalhes bibliográficos
Autor(a) principal: Myburgh, Jacobus
Data de Publicação: 2024
Outros Autores: Calitz, Anné
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Laticínios Cândido Tostes
Texto Completo: https://www.revistadoilct.com.br/rilct/article/view/948
Resumo: In this study, yoghurt fermentation time was accelerated by using the proteolytic enzyme Flavourzyme to produce sufficient peptides. Flavourzyme was added directly to the milk used for fermentation at different concentrations (0.1 g/L, 0.01 g/L, and 0.001g/L), and before heat treatment (90˚C for 15 min) where Flavourzyme was inactivated. The liberation of peptides during Flavourzyme pre-treatment was confirmed by RP-HPLC peptide profiling. It was possible to accelerate the fermentation time by 13.33% with Flavourzyme. The yoghurt made with Flavourzyme pre-treated milk did not demonstrate any significant quality changes. However, consumers were able to detect a difference between yoghurt made with Flavourzyme pre-treated milk and yoghurt made with untreated milk (control). Yoghurt made with Flavourzyme pre-treated milk scored lower in taste, texture, appearance, and overall acceptability, but was generally not rejected. Accelerated fermentation times could lead to economic advantages in the yoghurt manufacturing industry.
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spelling The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milkThe acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milkflavourzyme; protease; consumer satisfaction.flavourzyme; protease; satisfação do consumidor.In this study, yoghurt fermentation time was accelerated by using the proteolytic enzyme Flavourzyme to produce sufficient peptides. Flavourzyme was added directly to the milk used for fermentation at different concentrations (0.1 g/L, 0.01 g/L, and 0.001g/L), and before heat treatment (90˚C for 15 min) where Flavourzyme was inactivated. The liberation of peptides during Flavourzyme pre-treatment was confirmed by RP-HPLC peptide profiling. It was possible to accelerate the fermentation time by 13.33% with Flavourzyme. The yoghurt made with Flavourzyme pre-treated milk did not demonstrate any significant quality changes. However, consumers were able to detect a difference between yoghurt made with Flavourzyme pre-treated milk and yoghurt made with untreated milk (control). Yoghurt made with Flavourzyme pre-treated milk scored lower in taste, texture, appearance, and overall acceptability, but was generally not rejected. Accelerated fermentation times could lead to economic advantages in the yoghurt manufacturing industry.Neste estudo, o tempo de fermentação do iogurte foi acelerado usando a enzima proteolítica Flavourzyme para produzir peptídeos suficientes. Flavourzyme foi adicionado diretamente ao leite utilizado para fermentação em diferentes concentrações (0,1 g/L, 0,01 g/L e 0,001 g/L) e antes do tratamento térmico (90˚C por 15 min), onde o Flavourzyme foi inativado. A libertação de peptídeos durante o pré-tratamento com Flavourzyme foi confirmada pelo perfil peptídico de RP-HPLC. Foi possível acelerar o tempo de fermentação em 13,33% com Flavourzyme. O iogurte feito com leite pré-tratado Flavourzyme não apresentou alterações significativas na qualidade. No entanto, os consumidores conseguiram detectar uma diferença entre o iogurte feito com leite pré-tratado Flavourzyme e o iogurte feito com leite não tratado (controle). O iogurte feito com leite pré-tratado Flavourzyme obteve menor pontuação em sabor, textura, aparência e aceitabilidade geral, mas geralmente não foi rejeitado. Tempos de fermentação acelerados podem levar a vantagens econômicas na indústria de produção de iogurte.ILCTMyburgh, JacobusCalitz, Anné2024-08-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistadoilct.com.br/rilct/article/view/94810.14295/2238-6416.v77i3.948Journal of Candido Tostes Dairy Institute; v. 78, n. 3 (2023); 93-101Revista do Instituto de Laticínios Cândido Tostes; v. 78, n. 3 (2023); 93-1012238-64160100-3674reponame:Revista do Instituto de Laticínios Cândido Tostesinstname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)instacron:EPAMIGenghttps://www.revistadoilct.com.br/rilct/article/view/948/607https://www.revistadoilct.com.br/rilct/article/downloadSuppFile/948/559Direitos autorais 2024 Revista do Instituto de Laticínios Cândido Tosteshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2024-08-28T19:53:34Zoai:oai.rilct.emnuvens.com.br:article/948Revistahttp://www.revistadoilct.com.br/ONGhttps://www.revistadoilct.com.br/rilct/oai||revistadoilct@epamig.br|| revistadoilct@oi.com.br2238-64160100-3674opendoar:2024-08-28T19:53:34Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)false
dc.title.none.fl_str_mv The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
title The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
spellingShingle The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
Myburgh, Jacobus
flavourzyme; protease; consumer satisfaction.
flavourzyme; protease; satisfação do consumidor.
title_short The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
title_full The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
title_fullStr The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
title_full_unstemmed The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
title_sort The acceleration of yoghurt fermentation using peptides derived from proteolytic pre-treatment of milk
author Myburgh, Jacobus
author_facet Myburgh, Jacobus
Calitz, Anné
author_role author
author2 Calitz, Anné
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Myburgh, Jacobus
Calitz, Anné
dc.subject.none.fl_str_mv

dc.subject.por.fl_str_mv flavourzyme; protease; consumer satisfaction.
flavourzyme; protease; satisfação do consumidor.
topic flavourzyme; protease; consumer satisfaction.
flavourzyme; protease; satisfação do consumidor.
description In this study, yoghurt fermentation time was accelerated by using the proteolytic enzyme Flavourzyme to produce sufficient peptides. Flavourzyme was added directly to the milk used for fermentation at different concentrations (0.1 g/L, 0.01 g/L, and 0.001g/L), and before heat treatment (90˚C for 15 min) where Flavourzyme was inactivated. The liberation of peptides during Flavourzyme pre-treatment was confirmed by RP-HPLC peptide profiling. It was possible to accelerate the fermentation time by 13.33% with Flavourzyme. The yoghurt made with Flavourzyme pre-treated milk did not demonstrate any significant quality changes. However, consumers were able to detect a difference between yoghurt made with Flavourzyme pre-treated milk and yoghurt made with untreated milk (control). Yoghurt made with Flavourzyme pre-treated milk scored lower in taste, texture, appearance, and overall acceptability, but was generally not rejected. Accelerated fermentation times could lead to economic advantages in the yoghurt manufacturing industry.
publishDate 2024
dc.date.none.fl_str_mv 2024-08-23
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistadoilct.com.br/rilct/article/view/948
10.14295/2238-6416.v77i3.948
url https://www.revistadoilct.com.br/rilct/article/view/948
identifier_str_mv 10.14295/2238-6416.v77i3.948
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistadoilct.com.br/rilct/article/view/948/607
https://www.revistadoilct.com.br/rilct/article/downloadSuppFile/948/559
dc.rights.driver.fl_str_mv Direitos autorais 2024 Revista do Instituto de Laticínios Cândido Tostes
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2024 Revista do Instituto de Laticínios Cândido Tostes
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ILCT
publisher.none.fl_str_mv ILCT
dc.source.none.fl_str_mv Journal of Candido Tostes Dairy Institute; v. 78, n. 3 (2023); 93-101
Revista do Instituto de Laticínios Cândido Tostes; v. 78, n. 3 (2023); 93-101
2238-6416
0100-3674
reponame:Revista do Instituto de Laticínios Cândido Tostes
instname:Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)
instacron:EPAMIG
instname_str Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)
instacron_str EPAMIG
institution EPAMIG
reponame_str Revista do Instituto de Laticínios Cândido Tostes
collection Revista do Instituto de Laticínios Cândido Tostes
repository.name.fl_str_mv Revista do Instituto de Laticínios Cândido Tostes - Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG)
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