Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article

Detalhes bibliográficos
Autor(a) principal: Zotarelli Filho, Idiberto José
Data de Publicação: 2022
Outros Autores: Liedtke, Fernanda Soubhia, Ferrari, Rafaella Scalabrini, Daher, Isabelle Dalloul, Neiva, Eneidia Batista, Moura, Malú Inês Perez, Murad, Ana Cristyna Saad
Tipo de documento: Artigo
Idioma: eng
Título da fonte: MedNEXT Journal of Medical and Health Sciences
Texto Completo: https://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/241
Resumo: The present technical study presented the mechanical dissociation protocol of adipose tissue from manual liposuction, as well as showed the immunobiological characterization (immunophenotyping), cell viability of the stromal vascular fraction of human adipose tissue, and cell concentration per milliliter. It was observed that the technique of the present study obtained a characterization yield of the stromal vascular fraction of the adipose tissue in terms of cell viability, cell concentration per milliliter, and immunophenotyping comparable and superior to most published studies with good scientific evidence.
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spelling Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original articleAdipose tissueStromal vascular fractionMechanical techniqueOptimizationThe present technical study presented the mechanical dissociation protocol of adipose tissue from manual liposuction, as well as showed the immunobiological characterization (immunophenotyping), cell viability of the stromal vascular fraction of human adipose tissue, and cell concentration per milliliter. It was observed that the technique of the present study obtained a characterization yield of the stromal vascular fraction of the adipose tissue in terms of cell viability, cell concentration per milliliter, and immunophenotyping comparable and superior to most published studies with good scientific evidence.Faceres2022-12-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticleapplication/pdfhttps://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/24110.54448/mdnt22412MedNEXT Journal of Medical and Health Sciences; Vol. 3 No. 4 (2022): MedNEXT - November 2022MedNEXT Journal of Medical and Health Sciences; v. 3 n. 4 (2022): MedNEXT - November 20222763-567810.54448/mdnt224reponame:MedNEXT Journal of Medical and Health Sciencesinstname:Faculdade de Medicina em São José do Rio Preto (Faceres)instacron:FACERESenghttps://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/241/229Copyright (c) 2022 Idiberto José Zotarelli Filho, Fernanda Soubhia Liedtke, Rafaella Scalabrini Ferrari, Isabelle Dalloul Daher, Eneidia Batista Neiva, Malú Inês Perez Moura, Ana Cristyna Saad Muradhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessZotarelli Filho, Idiberto JoséLiedtke, Fernanda SoubhiaFerrari, Rafaella ScalabriniDaher, Isabelle DalloulNeiva, Eneidia BatistaMoura, Malú Inês PerezMurad, Ana Cristyna Saad2022-12-26T20:51:35Zoai:ojs2.mednext.zotarellifilhoscientificworks.com:article/241Revistahttps://mednext.zotarellifilhoscientificworks.com/index.php/mednextPUBhttps://mednext.zotarellifilhoscientificworks.com/index.php/mednext/oaimednextjmhs@zotarellifilhoscientificworks.com2763-56782763-5678opendoar:2022-12-26T20:51:35MedNEXT Journal of Medical and Health Sciences - Faculdade de Medicina em São José do Rio Preto (Faceres)false
dc.title.none.fl_str_mv Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
title Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
spellingShingle Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
Zotarelli Filho, Idiberto José
Adipose tissue
Stromal vascular fraction
Mechanical technique
Optimization
title_short Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
title_full Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
title_fullStr Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
title_full_unstemmed Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
title_sort Protocol for obtaining and characterizing the stromal vascular fraction of human adipose tissue by mechanical dissociation: an original article
author Zotarelli Filho, Idiberto José
author_facet Zotarelli Filho, Idiberto José
Liedtke, Fernanda Soubhia
Ferrari, Rafaella Scalabrini
Daher, Isabelle Dalloul
Neiva, Eneidia Batista
Moura, Malú Inês Perez
Murad, Ana Cristyna Saad
author_role author
author2 Liedtke, Fernanda Soubhia
Ferrari, Rafaella Scalabrini
Daher, Isabelle Dalloul
Neiva, Eneidia Batista
Moura, Malú Inês Perez
Murad, Ana Cristyna Saad
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zotarelli Filho, Idiberto José
Liedtke, Fernanda Soubhia
Ferrari, Rafaella Scalabrini
Daher, Isabelle Dalloul
Neiva, Eneidia Batista
Moura, Malú Inês Perez
Murad, Ana Cristyna Saad
dc.subject.por.fl_str_mv Adipose tissue
Stromal vascular fraction
Mechanical technique
Optimization
topic Adipose tissue
Stromal vascular fraction
Mechanical technique
Optimization
description The present technical study presented the mechanical dissociation protocol of adipose tissue from manual liposuction, as well as showed the immunobiological characterization (immunophenotyping), cell viability of the stromal vascular fraction of human adipose tissue, and cell concentration per milliliter. It was observed that the technique of the present study obtained a characterization yield of the stromal vascular fraction of the adipose tissue in terms of cell viability, cell concentration per milliliter, and immunophenotyping comparable and superior to most published studies with good scientific evidence.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/241
10.54448/mdnt22412
url https://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/241
identifier_str_mv 10.54448/mdnt22412
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/241/229
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faceres
publisher.none.fl_str_mv Faceres
dc.source.none.fl_str_mv MedNEXT Journal of Medical and Health Sciences; Vol. 3 No. 4 (2022): MedNEXT - November 2022
MedNEXT Journal of Medical and Health Sciences; v. 3 n. 4 (2022): MedNEXT - November 2022
2763-5678
10.54448/mdnt224
reponame:MedNEXT Journal of Medical and Health Sciences
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collection MedNEXT Journal of Medical and Health Sciences
repository.name.fl_str_mv MedNEXT Journal of Medical and Health Sciences - Faculdade de Medicina em São José do Rio Preto (Faceres)
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