Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Poultry Science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006 |
Resumo: | Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers. |
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Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementationFeed nutritionglutamineintestinal mucosamucosa growthornitine decarboxylaseSix hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.Fundacao de Apoio a Ciência e Tecnologia Avicolas2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006Brazilian Journal of Poultry Science v.9 n.2 2007reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/S1516-635X2007000200006info:eu-repo/semantics/openAccessFischer da Silva,AVBorges,SAMaiorka,AGivisiez,PENRocha,CMacari,Meng2007-10-09T00:00:00Zoai:scielo:S1516-635X2007000200006Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2007-10-09T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false |
dc.title.none.fl_str_mv |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
spellingShingle |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation Fischer da Silva,AV Feed nutrition glutamine intestinal mucosa mucosa growth ornitine decarboxylase |
title_short |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_full |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_fullStr |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_full_unstemmed |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_sort |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
author |
Fischer da Silva,AV |
author_facet |
Fischer da Silva,AV Borges,SA Maiorka,A Givisiez,PEN Rocha,C Macari,M |
author_role |
author |
author2 |
Borges,SA Maiorka,A Givisiez,PEN Rocha,C Macari,M |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fischer da Silva,AV Borges,SA Maiorka,A Givisiez,PEN Rocha,C Macari,M |
dc.subject.por.fl_str_mv |
Feed nutrition glutamine intestinal mucosa mucosa growth ornitine decarboxylase |
topic |
Feed nutrition glutamine intestinal mucosa mucosa growth ornitine decarboxylase |
description |
Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-635X2007000200006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
publisher.none.fl_str_mv |
Fundacao de Apoio a Ciência e Tecnologia Avicolas |
dc.source.none.fl_str_mv |
Brazilian Journal of Poultry Science v.9 n.2 2007 reponame:Brazilian Journal of Poultry Science (Online) instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) instacron:FACTA |
instname_str |
Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
instacron_str |
FACTA |
institution |
FACTA |
reponame_str |
Brazilian Journal of Poultry Science (Online) |
collection |
Brazilian Journal of Poultry Science (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA) |
repository.mail.fl_str_mv |
||rvfacta@terra.com.br |
_version_ |
1754122511349448704 |