Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation

Detalhes bibliográficos
Autor(a) principal: Fischer da Silva,AV
Data de Publicação: 2007
Outros Autores: Borges,SA, Maiorka,A, Givisiez,PEN, Rocha,C, Macari,M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Poultry Science (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006
Resumo: Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.
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spelling Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementationFeed nutritionglutamineintestinal mucosamucosa growthornitine decarboxylaseSix hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.Fundacao de Apoio a Ciência e Tecnologia Avicolas2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006Brazilian Journal of Poultry Science v.9 n.2 2007reponame:Brazilian Journal of Poultry Science (Online)instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)instacron:FACTA10.1590/S1516-635X2007000200006info:eu-repo/semantics/openAccessFischer da Silva,AVBorges,SAMaiorka,AGivisiez,PENRocha,CMacari,Meng2007-10-09T00:00:00Zoai:scielo:S1516-635X2007000200006Revistahttp://www.scielo.br/rbcahttps://old.scielo.br/oai/scielo-oai.php||rvfacta@terra.com.br1806-90611516-635Xopendoar:2007-10-09T00:00Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)false
dc.title.none.fl_str_mv Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
spellingShingle Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
Fischer da Silva,AV
Feed nutrition
glutamine
intestinal mucosa
mucosa growth
ornitine decarboxylase
title_short Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_full Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_fullStr Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_full_unstemmed Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_sort Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
author Fischer da Silva,AV
author_facet Fischer da Silva,AV
Borges,SA
Maiorka,A
Givisiez,PEN
Rocha,C
Macari,M
author_role author
author2 Borges,SA
Maiorka,A
Givisiez,PEN
Rocha,C
Macari,M
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fischer da Silva,AV
Borges,SA
Maiorka,A
Givisiez,PEN
Rocha,C
Macari,M
dc.subject.por.fl_str_mv Feed nutrition
glutamine
intestinal mucosa
mucosa growth
ornitine decarboxylase
topic Feed nutrition
glutamine
intestinal mucosa
mucosa growth
ornitine decarboxylase
description Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2007000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-635X2007000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
publisher.none.fl_str_mv Fundacao de Apoio a Ciência e Tecnologia Avicolas
dc.source.none.fl_str_mv Brazilian Journal of Poultry Science v.9 n.2 2007
reponame:Brazilian Journal of Poultry Science (Online)
instname:Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron:FACTA
instname_str Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
instacron_str FACTA
institution FACTA
reponame_str Brazilian Journal of Poultry Science (Online)
collection Brazilian Journal of Poultry Science (Online)
repository.name.fl_str_mv Brazilian Journal of Poultry Science (Online) - Fundação APINCO de Ciência e Tecnologia Avícolas (FACTA)
repository.mail.fl_str_mv ||rvfacta@terra.com.br
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