Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation

Detalhes bibliográficos
Autor(a) principal: Fischer da Silva, AV
Data de Publicação: 2007
Outros Autores: Borges, SA, Maiorka, A, Givisiez, PEN [UNESP], Rocha, C, Macari, Marcos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-635X2007000200006
http://hdl.handle.net/11449/2678
Resumo: Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.
id UNSP_8c1e9c9cc3293a57f9098d8fea00ca4f
oai_identifier_str oai:repositorio.unesp.br:11449/2678
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementationFeed nutritionglutamineintestinal mucosamucosa growthornitine decarboxylaseSix hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.UFPR Dept. FisiologiaUFPR Dept. ZootecniaUTP Faculdade de Ciências Biológicas e da SaúdeUNESP FCAVUNESP FCAVFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Federal do Paraná (UFPR)UTP Faculdade de Ciências Biológicas e da SaúdeUniversidade Estadual Paulista (Unesp)Fischer da Silva, AVBorges, SAMaiorka, AGivisiez, PEN [UNESP]Rocha, CMacari, Marcos [UNESP]2014-05-20T13:15:33Z2014-05-20T13:15:33Z2007-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article111-115application/pdfhttp://dx.doi.org/10.1590/S1516-635X2007000200006Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 2, p. 111-115, 2007.1516-635Xhttp://hdl.handle.net/11449/267810.1590/S1516-635X2007000200006S1516-635X2007000200006WOS:000257537600006S1516-635X2007000200006.pdf5713558572926669SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2024-06-06T18:41:55Zoai:repositorio.unesp.br:11449/2678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:12:09.039727Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
spellingShingle Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
Fischer da Silva, AV
Feed nutrition
glutamine
intestinal mucosa
mucosa growth
ornitine decarboxylase
title_short Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_full Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_fullStr Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_full_unstemmed Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
title_sort Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
author Fischer da Silva, AV
author_facet Fischer da Silva, AV
Borges, SA
Maiorka, A
Givisiez, PEN [UNESP]
Rocha, C
Macari, Marcos [UNESP]
author_role author
author2 Borges, SA
Maiorka, A
Givisiez, PEN [UNESP]
Rocha, C
Macari, Marcos [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Paraná (UFPR)
UTP Faculdade de Ciências Biológicas e da Saúde
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fischer da Silva, AV
Borges, SA
Maiorka, A
Givisiez, PEN [UNESP]
Rocha, C
Macari, Marcos [UNESP]
dc.subject.por.fl_str_mv Feed nutrition
glutamine
intestinal mucosa
mucosa growth
ornitine decarboxylase
topic Feed nutrition
glutamine
intestinal mucosa
mucosa growth
ornitine decarboxylase
description Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
2014-05-20T13:15:33Z
2014-05-20T13:15:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-635X2007000200006
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 2, p. 111-115, 2007.
1516-635X
http://hdl.handle.net/11449/2678
10.1590/S1516-635X2007000200006
S1516-635X2007000200006
WOS:000257537600006
S1516-635X2007000200006.pdf
5713558572926669
url http://dx.doi.org/10.1590/S1516-635X2007000200006
http://hdl.handle.net/11449/2678
identifier_str_mv Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 2, p. 111-115, 2007.
1516-635X
10.1590/S1516-635X2007000200006
S1516-635X2007000200006
WOS:000257537600006
S1516-635X2007000200006.pdf
5713558572926669
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Ciência Avícola
0.463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 111-115
application/pdf
dc.publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129170587254784