Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-635X2007000200006 http://hdl.handle.net/11449/2678 |
Resumo: | Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers. |
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Repositório Institucional da UNESP |
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Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementationFeed nutritionglutamineintestinal mucosamucosa growthornitine decarboxylaseSix hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers.UFPR Dept. FisiologiaUFPR Dept. ZootecniaUTP Faculdade de Ciências Biológicas e da SaúdeUNESP FCAVUNESP FCAVFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Federal do Paraná (UFPR)UTP Faculdade de Ciências Biológicas e da SaúdeUniversidade Estadual Paulista (Unesp)Fischer da Silva, AVBorges, SAMaiorka, AGivisiez, PEN [UNESP]Rocha, CMacari, Marcos [UNESP]2014-05-20T13:15:33Z2014-05-20T13:15:33Z2007-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article111-115application/pdfhttp://dx.doi.org/10.1590/S1516-635X2007000200006Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 2, p. 111-115, 2007.1516-635Xhttp://hdl.handle.net/11449/267810.1590/S1516-635X2007000200006S1516-635X2007000200006WOS:000257537600006S1516-635X2007000200006.pdf5713558572926669SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2024-06-06T18:41:55Zoai:repositorio.unesp.br:11449/2678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:12:09.039727Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
spellingShingle |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation Fischer da Silva, AV Feed nutrition glutamine intestinal mucosa mucosa growth ornitine decarboxylase |
title_short |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_full |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_fullStr |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_full_unstemmed |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
title_sort |
Ornithine decarboxylase expression in the small intestine of broilers submitted to feed restriction and glutamine supplementation |
author |
Fischer da Silva, AV |
author_facet |
Fischer da Silva, AV Borges, SA Maiorka, A Givisiez, PEN [UNESP] Rocha, C Macari, Marcos [UNESP] |
author_role |
author |
author2 |
Borges, SA Maiorka, A Givisiez, PEN [UNESP] Rocha, C Macari, Marcos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) UTP Faculdade de Ciências Biológicas e da Saúde Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Fischer da Silva, AV Borges, SA Maiorka, A Givisiez, PEN [UNESP] Rocha, C Macari, Marcos [UNESP] |
dc.subject.por.fl_str_mv |
Feed nutrition glutamine intestinal mucosa mucosa growth ornitine decarboxylase |
topic |
Feed nutrition glutamine intestinal mucosa mucosa growth ornitine decarboxylase |
description |
Six hundred and forty one-day-old Cobb male broilers were used to evaluate ornithine decarboxylase (ODC) expression in the mucosa of the small intestine. Birds were submitted to early feed restriction from 7 to 14 days of age. The provided feed was supplemented with glutamine. A completely randomized design with a 2 x 2 factorial arrangement was used (with or without glutamine, with or without feed restriction). Restricted-fed birds were fed at 30% the amount of the ad libitum fed group from 7 to 14 days of age. Glutamine was added at the level of 1% in the diet supplied from 1 to 28 days of age. Protein concentration in the small intestine mucosa was determined, and ODC expression at 7, 14, 21, and 28 days of age was evaluated by dot blotting. ODC was present in the mucosa of broilers, and the presence of glutamine in the diet increased ODC activation. Glutamine prevented mucosa atrophy by stimulating protein synthesis, and was effective against the effects of feed restriction. Dot blotting can be used to quantify ODC expression in the intestinal mucosa of broilers. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 2014-05-20T13:15:33Z 2014-05-20T13:15:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-635X2007000200006 Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 2, p. 111-115, 2007. 1516-635X http://hdl.handle.net/11449/2678 10.1590/S1516-635X2007000200006 S1516-635X2007000200006 WOS:000257537600006 S1516-635X2007000200006.pdf 5713558572926669 |
url |
http://dx.doi.org/10.1590/S1516-635X2007000200006 http://hdl.handle.net/11449/2678 |
identifier_str_mv |
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 2, p. 111-115, 2007. 1516-635X 10.1590/S1516-635X2007000200006 S1516-635X2007000200006 WOS:000257537600006 S1516-635X2007000200006.pdf 5713558572926669 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ciência Avícola 0.463 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
111-115 application/pdf |
dc.publisher.none.fl_str_mv |
Fundação APINCO de Ciência e Tecnologia Avícolas |
publisher.none.fl_str_mv |
Fundação APINCO de Ciência e Tecnologia Avícolas |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129170587254784 |