Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biota Neotropica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300029 |
Resumo: | Molecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analyzed as if they were orthologous sequences. In this contribution the different points of view regarding the implications of mitochondrial pseudogenes for entomology are reviewed and discussed. A discussion of the problem from a historical and conceptual perspective is presented as well as a discussion of strategies to keep these nuclear mtDNA copies out of sequence analyzes. |
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Biota Neotropica |
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Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issueCOImolecularNUMTsMolecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analyzed as if they were orthologous sequences. In this contribution the different points of view regarding the implications of mitochondrial pseudogenes for entomology are reviewed and discussed. A discussion of the problem from a historical and conceptual perspective is presented as well as a discussion of strategies to keep these nuclear mtDNA copies out of sequence analyzes.Instituto Virtual da Biodiversidade | BIOTA - FAPESP2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300029Biota Neotropica v.12 n.3 2012reponame:Biota Neotropicainstname:Instituto Virtual da Biodiversidade (BIOTA-FAPESP)instacron:BIOTA - FAPESP10.1590/S1676-06032012000300029info:eu-repo/semantics/openAccessLeite,Luis Anderson Ribeiroeng2012-11-08T00:00:00Zoai:scielo:S1676-06032012000300029Revistahttps://www.biotaneotropica.org.br/v20n1/pt/https://old.scielo.br/oai/scielo-oai.php||juliosa@unifap.br1676-06111676-0611opendoar:2012-11-08T00:00Biota Neotropica - Instituto Virtual da Biodiversidade (BIOTA-FAPESP)false |
dc.title.none.fl_str_mv |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
title |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
spellingShingle |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue Leite,Luis Anderson Ribeiro COI molecular NUMTs |
title_short |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
title_full |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
title_fullStr |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
title_full_unstemmed |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
title_sort |
Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue |
author |
Leite,Luis Anderson Ribeiro |
author_facet |
Leite,Luis Anderson Ribeiro |
author_role |
author |
dc.contributor.author.fl_str_mv |
Leite,Luis Anderson Ribeiro |
dc.subject.por.fl_str_mv |
COI molecular NUMTs |
topic |
COI molecular NUMTs |
description |
Molecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analyzed as if they were orthologous sequences. In this contribution the different points of view regarding the implications of mitochondrial pseudogenes for entomology are reviewed and discussed. A discussion of the problem from a historical and conceptual perspective is presented as well as a discussion of strategies to keep these nuclear mtDNA copies out of sequence analyzes. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300029 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300029 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-06032012000300029 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
publisher.none.fl_str_mv |
Instituto Virtual da Biodiversidade | BIOTA - FAPESP |
dc.source.none.fl_str_mv |
Biota Neotropica v.12 n.3 2012 reponame:Biota Neotropica instname:Instituto Virtual da Biodiversidade (BIOTA-FAPESP) instacron:BIOTA - FAPESP |
instname_str |
Instituto Virtual da Biodiversidade (BIOTA-FAPESP) |
instacron_str |
BIOTA - FAPESP |
institution |
BIOTA - FAPESP |
reponame_str |
Biota Neotropica |
collection |
Biota Neotropica |
repository.name.fl_str_mv |
Biota Neotropica - Instituto Virtual da Biodiversidade (BIOTA-FAPESP) |
repository.mail.fl_str_mv |
||juliosa@unifap.br |
_version_ |
1754575899033862144 |