First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Revista brasileira de ginecologia e obstetrícia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021001100878 |
Resumo: | Abstract Non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) aiming to assess cell-free embryonic DNA in spent culturemedia is promising, especially because it might overcome the diminished rates of implantation caused by the inadequate performance of trophectoderm (TE) biopsy. Our center is part of the largest study to date assessing the concordance between conventional PGT-A and niPGT-A, and we report here the delivery of the first baby born in Brazil using niPGT-A. The parents of the baby were admitted to our center in 2018. They did not present history of infertility, and they were interested in using in vitro fertilization (IVF) and PGT-A in order to avoid congenital anomalies in the offspring. A total of 11 (3 day-5 and 8 day-6) expanded blastocysts were biopsied, and the spent culture media (culture from day-4 to day-6) from 8 day-6 blastocysts were collected for niPGT-A. Overall, 7 embryos yielded informative results for trophectoderm (TE) and media samples. Among the embryos with informative results, 5 presented concordant diagnosis between conventional PGTA and niPGT-A, and 2 presented discordant diagnosis (1 false-positive and one falsenegative). The Blastocyst 4, diagnosed as 46, XY by both niPGT-A and conventional PGTA, was warmed up and transferred, resulting in the birth of a healthy 3.8 kg boy in February 2020. Based on our results and the recent literature, we believe that the safest current application of niPGT-A would be as a method of embryo selection for patients without an indication for conventional PGT-A. The approximate 80% of reliability of niPGT-A in the diagnosis of ploidy is superior to predictions provided by other noninvasive approaches like morphology and morphokinetics selection. |
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First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-AniPGT-Aspent culture mediaPGT-Atrophectoderm biopsyblastocystAbstract Non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) aiming to assess cell-free embryonic DNA in spent culturemedia is promising, especially because it might overcome the diminished rates of implantation caused by the inadequate performance of trophectoderm (TE) biopsy. Our center is part of the largest study to date assessing the concordance between conventional PGT-A and niPGT-A, and we report here the delivery of the first baby born in Brazil using niPGT-A. The parents of the baby were admitted to our center in 2018. They did not present history of infertility, and they were interested in using in vitro fertilization (IVF) and PGT-A in order to avoid congenital anomalies in the offspring. A total of 11 (3 day-5 and 8 day-6) expanded blastocysts were biopsied, and the spent culture media (culture from day-4 to day-6) from 8 day-6 blastocysts were collected for niPGT-A. Overall, 7 embryos yielded informative results for trophectoderm (TE) and media samples. Among the embryos with informative results, 5 presented concordant diagnosis between conventional PGTA and niPGT-A, and 2 presented discordant diagnosis (1 false-positive and one falsenegative). The Blastocyst 4, diagnosed as 46, XY by both niPGT-A and conventional PGTA, was warmed up and transferred, resulting in the birth of a healthy 3.8 kg boy in February 2020. Based on our results and the recent literature, we believe that the safest current application of niPGT-A would be as a method of embryo selection for patients without an indication for conventional PGT-A. The approximate 80% of reliability of niPGT-A in the diagnosis of ploidy is superior to predictions provided by other noninvasive approaches like morphology and morphokinetics selection.Federação Brasileira das Sociedades de Ginecologia e Obstetrícia2021-11-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021001100878Revista Brasileira de Ginecologia e Obstetrícia v.43 n.11 2021reponame:Revista brasileira de ginecologia e obstetrícia (Online)instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)instacron:FEBRASGO10.1055/s-0041-1736302info:eu-repo/semantics/openAccessKulmann,Marcos Iuri RoosRiboldi,MárciaMartello,CarolinaBos-Mikich,AdrianaFrantz,GertaDutra,CarolineDonatti,Luiza MezzomoOliveira,NormaFrantz,Niloeng2022-01-10T00:00:00Zoai:scielo:S0100-72032021001100878Revistahttp://www.scielo.br/rbgohttps://old.scielo.br/oai/scielo-oai.phppublicações@febrasgo.org.br||rbgo@fmrp.usp.br1806-93390100-7203opendoar:2022-01-10T00:00Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO)false |
dc.title.none.fl_str_mv |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
title |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
spellingShingle |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A Kulmann,Marcos Iuri Roos niPGT-A spent culture media PGT-A trophectoderm biopsy blastocyst |
title_short |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
title_full |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
title_fullStr |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
title_full_unstemmed |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
title_sort |
First Baby Born in Brazil after Simultaneous Diagnosis through Non-Invasive and Conventional PGT-A |
author |
Kulmann,Marcos Iuri Roos |
author_facet |
Kulmann,Marcos Iuri Roos Riboldi,Márcia Martello,Carolina Bos-Mikich,Adriana Frantz,Gerta Dutra,Caroline Donatti,Luiza Mezzomo Oliveira,Norma Frantz,Nilo |
author_role |
author |
author2 |
Riboldi,Márcia Martello,Carolina Bos-Mikich,Adriana Frantz,Gerta Dutra,Caroline Donatti,Luiza Mezzomo Oliveira,Norma Frantz,Nilo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Kulmann,Marcos Iuri Roos Riboldi,Márcia Martello,Carolina Bos-Mikich,Adriana Frantz,Gerta Dutra,Caroline Donatti,Luiza Mezzomo Oliveira,Norma Frantz,Nilo |
dc.subject.por.fl_str_mv |
niPGT-A spent culture media PGT-A trophectoderm biopsy blastocyst |
topic |
niPGT-A spent culture media PGT-A trophectoderm biopsy blastocyst |
description |
Abstract Non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) aiming to assess cell-free embryonic DNA in spent culturemedia is promising, especially because it might overcome the diminished rates of implantation caused by the inadequate performance of trophectoderm (TE) biopsy. Our center is part of the largest study to date assessing the concordance between conventional PGT-A and niPGT-A, and we report here the delivery of the first baby born in Brazil using niPGT-A. The parents of the baby were admitted to our center in 2018. They did not present history of infertility, and they were interested in using in vitro fertilization (IVF) and PGT-A in order to avoid congenital anomalies in the offspring. A total of 11 (3 day-5 and 8 day-6) expanded blastocysts were biopsied, and the spent culture media (culture from day-4 to day-6) from 8 day-6 blastocysts were collected for niPGT-A. Overall, 7 embryos yielded informative results for trophectoderm (TE) and media samples. Among the embryos with informative results, 5 presented concordant diagnosis between conventional PGTA and niPGT-A, and 2 presented discordant diagnosis (1 false-positive and one falsenegative). The Blastocyst 4, diagnosed as 46, XY by both niPGT-A and conventional PGTA, was warmed up and transferred, resulting in the birth of a healthy 3.8 kg boy in February 2020. Based on our results and the recent literature, we believe that the safest current application of niPGT-A would be as a method of embryo selection for patients without an indication for conventional PGT-A. The approximate 80% of reliability of niPGT-A in the diagnosis of ploidy is superior to predictions provided by other noninvasive approaches like morphology and morphokinetics selection. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021001100878 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032021001100878 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1055/s-0041-1736302 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
publisher.none.fl_str_mv |
Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
dc.source.none.fl_str_mv |
Revista Brasileira de Ginecologia e Obstetrícia v.43 n.11 2021 reponame:Revista brasileira de ginecologia e obstetrícia (Online) instname:Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) instacron:FEBRASGO |
instname_str |
Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) |
instacron_str |
FEBRASGO |
institution |
FEBRASGO |
reponame_str |
Revista brasileira de ginecologia e obstetrícia (Online) |
collection |
Revista brasileira de ginecologia e obstetrícia (Online) |
repository.name.fl_str_mv |
Revista brasileira de ginecologia e obstetrícia (Online) - Federação Brasileira das Sociedades de Ginecologia e Obstetrícia (FEBRASGO) |
repository.mail.fl_str_mv |
publicações@febrasgo.org.br||rbgo@fmrp.usp.br |
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1754115945898442752 |