Coumarin determination in Commercial Extracts of Guaco: A Case Study of the Quality Control of Phytotherapics

• Autor(a) principal: Kaiser, C. R.
• Data de Publicação: 2007
• Outros Autores: Santos, A. R., Abreu, A. S.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/67
• topic: Phytotherapics; Quality Control; Guaco; Coumarin; Fitoterápicos; Controle de Qualidade; Guaco; Cumarina

Phytotherapics need a quality control of production (planting, drying and storage) and the finish product commercialization. For that, it is necessary to get the results of qualitative and quantitative analysis of the active compounds, showing that there was no degradation and lost of pharmacological properties. This article describe a selective method of extraction and coumarin quantification in commercial samples of guaco by gas chromatography (GC), commercialized as Mikania glomerata Sprengel. For this end, seven commercial samples were used from different States of Brazil. The results were confronted with the rules of the National Health Surveillance Agency (ANVISA), Brazilian agency that regulates and registers medicines, which recommends a daily maximum dose of 4.89 mg. Guaco is an important bronchodilatator used in Brazil, whose biological activity comes from coumarin. The commercial samples of guaco were according to ANVISA’s Recommendations. However, the wide range of coumarin noticed (1.41 mg to 4.48 mg for leaf gram) shows that the commercial samples need to be standardized, getting a safe consumption of this phytotherapic. The method allowed a fast and simple quantification of coumarin in commercial samples of guaco, showing a recovery of 90.22 ± 0.32%.