Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio

Detalhes bibliográficos
Autor(a) principal: Wolkers, Carla Patrícia Bejo
Data de Publicação: 2023
Outros Autores: Pinheiro, Letícia Rodrigues
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Fitos
Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479
Resumo: Pain is characterized as an unpleasant sensory and emotional experience associated with tissue damage or injury, being detected through specialized receptors called nociceptors. Antinociception is the process where the transmission of nociceptive information is inhibited, being mediated by endogenous systems and achieved by exogenous substances, such as drugs. The research for new pharmacological compounds for pain treatment is a wide field of study, therefore, the present work aimed evaluate a new biological model using the fish D. rerio to analyze the antinociceptive potential of substances extracted from plants. Twenty-four fish were filmed for 5 min (baseline), treated with vehicle (Control, n=8), C. citratus essential oil (200 mg/kg, n=8) or dipyrone (100 mg/kg, n=8), after 30 minutes the animals were submitted to the formalin nociceptive test and filmed for 5 min (post-stimulus) for behavioral evaluation. Decreased locomotor activity was observed in response to formalin, and this effect was blocked by treatment with dipyrone. The essential oil (EO) did not present significant effects on nociception, requiring further studies to determine its analgesic potential.
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spelling Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerioUso do teste da formalina para avaliação do potencial antinociceptivo do óleo essencial de capim limão (Cymbopogon citratus) utilizando o peixe Danio rerioAntinociceptionPainNociceptionZebrafishAntinocicepçãoDorNocicepçãoZebrafishPain is characterized as an unpleasant sensory and emotional experience associated with tissue damage or injury, being detected through specialized receptors called nociceptors. Antinociception is the process where the transmission of nociceptive information is inhibited, being mediated by endogenous systems and achieved by exogenous substances, such as drugs. The research for new pharmacological compounds for pain treatment is a wide field of study, therefore, the present work aimed evaluate a new biological model using the fish D. rerio to analyze the antinociceptive potential of substances extracted from plants. Twenty-four fish were filmed for 5 min (baseline), treated with vehicle (Control, n=8), C. citratus essential oil (200 mg/kg, n=8) or dipyrone (100 mg/kg, n=8), after 30 minutes the animals were submitted to the formalin nociceptive test and filmed for 5 min (post-stimulus) for behavioral evaluation. Decreased locomotor activity was observed in response to formalin, and this effect was blocked by treatment with dipyrone. The essential oil (EO) did not present significant effects on nociception, requiring further studies to determine its analgesic potential.A dor é caracterizada como uma experiência sensorial e emocional desagradável associada a um dano ou lesão tecidual, sendo detectada por meio dos receptores especializados denominados nociceptores. A antinocicepção é o processo em que há a inibição da transmissão da informação nociceptiva, sendo mediada por sistemas endógenos e alcançada por substâncias exógenas, como os fármacos. A necessidade de se buscar novos compostos farmacológicos que auxiliam no tratamento da dor compõe um amplo campo de estudo, sendo assim, o presente trabalho objetivou testar um modelo biológico utilizando o peixe D. rerio para avaliação do potencial antinociceptivo de substâncias extraídas de plantas. Utilizou-se 24 peixes que foram filmados por 5 min (linha de base), tratados com veículo (Controle, n=8), óleo essencial de C. citratus (200 mg/kg, n=8) ou dipirona (100 mg/kg, n=8), após 30 minutos os animais foram submetidos ao teste nociceptivo da formalina e novamente filmados por 5 min (pós-estímulo), para avaliação comportamental. Foi observada diminuição da atividade locomotora em resposta à formalina, sendo este efeito bloqueado pelo tratamento com dipirona. O óleo essencial (OE) não apresentou efeitos significativos sobre a nocicepção, sendo necessários estudos complementares para a determinação de seu potencial antinociceptivo.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2023-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmltext/xmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/147910.32712/2446-4775.2023.1479Revista Fitos; Vol. 17 No. 3 (2023); 411-424Revista Fitos; Vol. 17 Núm. 3 (2023); 411-424Revista Fitos; v. 17 n. 3 (2023); 411-4242446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479/1381https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479/1382https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479/1452Copyright (c) 2023 Revista Fitoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess Wolkers, Carla Patrícia Bejo Pinheiro, Letícia Rodrigues2023-12-13T14:40:38Zoai:ojs.revistafitos.far.fiocruz.br:article/1479Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2023-12-13T14:40:38Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
Uso do teste da formalina para avaliação do potencial antinociceptivo do óleo essencial de capim limão (Cymbopogon citratus) utilizando o peixe Danio rerio
title Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
spellingShingle Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
Wolkers, Carla Patrícia Bejo
Antinociception
Pain
Nociception
Zebrafish
Antinocicepção
Dor
Nocicepção
Zebrafish
title_short Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
title_full Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
title_fullStr Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
title_full_unstemmed Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
title_sort Use of the formalin test to evaluate the antinociceptive potential of lemongrass essential oil (Cymbopogon citratus) using the fish Danio rerio
author Wolkers, Carla Patrícia Bejo
author_facet Wolkers, Carla Patrícia Bejo
Pinheiro, Letícia Rodrigues
author_role author
author2 Pinheiro, Letícia Rodrigues
author2_role author
dc.contributor.author.fl_str_mv Wolkers, Carla Patrícia Bejo
Pinheiro, Letícia Rodrigues
dc.subject.por.fl_str_mv Antinociception
Pain
Nociception
Zebrafish
Antinocicepção
Dor
Nocicepção
Zebrafish
topic Antinociception
Pain
Nociception
Zebrafish
Antinocicepção
Dor
Nocicepção
Zebrafish
description Pain is characterized as an unpleasant sensory and emotional experience associated with tissue damage or injury, being detected through specialized receptors called nociceptors. Antinociception is the process where the transmission of nociceptive information is inhibited, being mediated by endogenous systems and achieved by exogenous substances, such as drugs. The research for new pharmacological compounds for pain treatment is a wide field of study, therefore, the present work aimed evaluate a new biological model using the fish D. rerio to analyze the antinociceptive potential of substances extracted from plants. Twenty-four fish were filmed for 5 min (baseline), treated with vehicle (Control, n=8), C. citratus essential oil (200 mg/kg, n=8) or dipyrone (100 mg/kg, n=8), after 30 minutes the animals were submitted to the formalin nociceptive test and filmed for 5 min (post-stimulus) for behavioral evaluation. Decreased locomotor activity was observed in response to formalin, and this effect was blocked by treatment with dipyrone. The essential oil (EO) did not present significant effects on nociception, requiring further studies to determine its analgesic potential.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479
10.32712/2446-4775.2023.1479
url https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479
identifier_str_mv 10.32712/2446-4775.2023.1479
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479/1381
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479/1382
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1479/1452
dc.rights.driver.fl_str_mv Copyright (c) 2023 Revista Fitos
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Revista Fitos
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
text/xml
dc.publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
dc.source.none.fl_str_mv Revista Fitos; Vol. 17 No. 3 (2023); 411-424
Revista Fitos; Vol. 17 Núm. 3 (2023); 411-424
Revista Fitos; v. 17 n. 3 (2023); 411-424
2446-4775
1808-9569
reponame:Revista Fitos
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Revista Fitos
collection Revista Fitos
repository.name.fl_str_mv Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br
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