Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae)

• Autor(a) principal: Moreira, D. L.
• Data de Publicação: 2010
• Outros Autores: Fonseca, V. M., Bhering, C. A., Vasconcelos, M. F., Cunha-Junior, E. F., Canto-Cavalheiro, M. M., Torres-Santos, E. C., Kaplan, M. A. C.
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108
• topic: Piper cabralanum; Piperaceae; Antileishmanial Activity; Hydrocarbons; Sesquiterpenes; Steroids; Piper cabralanum; Piperaceae; Atividade Leishmanicida; Hidrocarbonetos; Sesquiterpenos; Esteróides

Leaves from Piper cabralanum C.DC. were collected in the city of Teresópolis / RJ, in particular site near the National Park of Serra dos Órgãos. The plant material was dried and then subjected to extraction with methanol by static maceration. The obtained methanol extract was concentrated and subjected to liquid-liquid partition with solvents of different polarities, yielding fractions soluble in n-hexane, dichloromethane, ethyl acetate and n-butanol. The crude methanol extract and its partitions were tested in promastigotes of Leishmania amazonensis. Only the crude methanol extract and its partition obtained with n-hexane (PCA-Hex) showed high activity against promastigotes (IC50 value = 30 μg/ml and 25 μg/ml, respectively). This partition was subjected to purification by column chromatography with silica gel, and the resulting non-polar fractions were analyzed by gas chromatography coupled to mass spectrometry, which allowed the characterization of hydrocarbons, sesquiterpenes, squalene and a mixture of steroids (campesterol, stigmasterol and b-sitosterol).