Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae)
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Fitos |
Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108 |
Resumo: | Leaves from Piper cabralanum C.DC. were collected in the city of Teresópolis / RJ, in particular site near the National Park of Serra dos Órgãos. The plant material was dried and then subjected to extraction with methanol by static maceration. The obtained methanol extract was concentrated and subjected to liquid-liquid partition with solvents of different polarities, yielding fractions soluble in n-hexane, dichloromethane, ethyl acetate and n-butanol. The crude methanol extract and its partitions were tested in promastigotes of Leishmania amazonensis. Only the crude methanol extract and its partition obtained with n-hexane (PCA-Hex) showed high activity against promastigotes (IC50 value = 30 μg/ml and 25 μg/ml, respectively). This partition was subjected to purification by column chromatography with silica gel, and the resulting non-polar fractions were analyzed by gas chromatography coupled to mass spectrometry, which allowed the characterization of hydrocarbons, sesquiterpenes, squalene and a mixture of steroids (campesterol, stigmasterol and b-sitosterol). |
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Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae)Estudo Químico e da Atividade Leishmanicida de Frações de Piper cabralanum C.DC. (Piperaceae)Piper cabralanumPiperaceaeAntileishmanial ActivityHydrocarbonsSesquiterpenesSteroidsPiper cabralanumPiperaceaeAtividade LeishmanicidaHidrocarbonetosSesquiterpenosEsteróidesLeaves from Piper cabralanum C.DC. were collected in the city of Teresópolis / RJ, in particular site near the National Park of Serra dos Órgãos. The plant material was dried and then subjected to extraction with methanol by static maceration. The obtained methanol extract was concentrated and subjected to liquid-liquid partition with solvents of different polarities, yielding fractions soluble in n-hexane, dichloromethane, ethyl acetate and n-butanol. The crude methanol extract and its partitions were tested in promastigotes of Leishmania amazonensis. Only the crude methanol extract and its partition obtained with n-hexane (PCA-Hex) showed high activity against promastigotes (IC50 value = 30 μg/ml and 25 μg/ml, respectively). This partition was subjected to purification by column chromatography with silica gel, and the resulting non-polar fractions were analyzed by gas chromatography coupled to mass spectrometry, which allowed the characterization of hydrocarbons, sesquiterpenes, squalene and a mixture of steroids (campesterol, stigmasterol and b-sitosterol).Folhas de Piper cabralanum C.DC. foram coletadas no Município de Teresópolis/RJ, em área particular próxima ao Parque Nacional da Serra dos Órgãos. O material vegetal foi seco em estufa a 40oC e, em seguida, submetido a extração com metanol por maceração estática. O extrato metanólico foi concentrado e particionado em solventes de diferentes polaridades, rendendo frações solúveis em n-hexano, diclorometano, acetato de etila e n-butanol. O extrato metanólico bruto e suas partições foram testadas em promastigotas de Leishmania amazonensis. Somente o extrato metanólico bruto e sua partição em n-hexano (PCA-Hex) mostraram boa atividade contra L. amazonensis (CE50 de 30 μg/ml e 25 μg/ml, respectivamente. Essa partição foi submetida à purificação por cromatografia em coluna com gel de sílica e posterior análise por cromatografia com fase gasosa acoplada à espectrometria de massas, o que possibilitou a caracterização de hidrocarbonetos, sesquiterpenos, esqualeno e uma mistura de esteróides (campesterol, estigmasterol e b-sitosterol).Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/10810.32712/2446-4775.2010.108Revista Fitos; Vol. 5 No. 01 (2010); 92-98Revista Fitos; Vol. 5 Núm. 01 (2010); 92-98Revista Fitos; v. 5 n. 01 (2010); 92-982446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108/107https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108/1212Copyright (c) 2010 Revista Fitos Eletrônicainfo:eu-repo/semantics/openAccessMoreira, D. L.Fonseca, V. M.Bhering, C. A.Vasconcelos, M. F.Cunha-Junior, E. F.Canto-Cavalheiro, M. M.Torres-Santos, E. C.Kaplan, M. A. C.2022-10-18T19:22:43Zoai:ojs.revistafitos.far.fiocruz.br:article/108Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2022-10-18T19:22:43Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) Estudo Químico e da Atividade Leishmanicida de Frações de Piper cabralanum C.DC. (Piperaceae) |
title |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) |
spellingShingle |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) Moreira, D. L. Piper cabralanum Piperaceae Antileishmanial Activity Hydrocarbons Sesquiterpenes Steroids Piper cabralanum Piperaceae Atividade Leishmanicida Hidrocarbonetos Sesquiterpenos Esteróides |
title_short |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) |
title_full |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) |
title_fullStr |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) |
title_full_unstemmed |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) |
title_sort |
Chemical Composition and Antileishmanial Activity from Fractions of Piper cabralanum C.DC. (Piperaceae) |
author |
Moreira, D. L. |
author_facet |
Moreira, D. L. Fonseca, V. M. Bhering, C. A. Vasconcelos, M. F. Cunha-Junior, E. F. Canto-Cavalheiro, M. M. Torres-Santos, E. C. Kaplan, M. A. C. |
author_role |
author |
author2 |
Fonseca, V. M. Bhering, C. A. Vasconcelos, M. F. Cunha-Junior, E. F. Canto-Cavalheiro, M. M. Torres-Santos, E. C. Kaplan, M. A. C. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Moreira, D. L. Fonseca, V. M. Bhering, C. A. Vasconcelos, M. F. Cunha-Junior, E. F. Canto-Cavalheiro, M. M. Torres-Santos, E. C. Kaplan, M. A. C. |
dc.subject.por.fl_str_mv |
Piper cabralanum Piperaceae Antileishmanial Activity Hydrocarbons Sesquiterpenes Steroids Piper cabralanum Piperaceae Atividade Leishmanicida Hidrocarbonetos Sesquiterpenos Esteróides |
topic |
Piper cabralanum Piperaceae Antileishmanial Activity Hydrocarbons Sesquiterpenes Steroids Piper cabralanum Piperaceae Atividade Leishmanicida Hidrocarbonetos Sesquiterpenos Esteróides |
description |
Leaves from Piper cabralanum C.DC. were collected in the city of Teresópolis / RJ, in particular site near the National Park of Serra dos Órgãos. The plant material was dried and then subjected to extraction with methanol by static maceration. The obtained methanol extract was concentrated and subjected to liquid-liquid partition with solvents of different polarities, yielding fractions soluble in n-hexane, dichloromethane, ethyl acetate and n-butanol. The crude methanol extract and its partitions were tested in promastigotes of Leishmania amazonensis. Only the crude methanol extract and its partition obtained with n-hexane (PCA-Hex) showed high activity against promastigotes (IC50 value = 30 μg/ml and 25 μg/ml, respectively). This partition was subjected to purification by column chromatography with silica gel, and the resulting non-polar fractions were analyzed by gas chromatography coupled to mass spectrometry, which allowed the characterization of hydrocarbons, sesquiterpenes, squalene and a mixture of steroids (campesterol, stigmasterol and b-sitosterol). |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108 10.32712/2446-4775.2010.108 |
url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108 |
identifier_str_mv |
10.32712/2446-4775.2010.108 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108/107 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/108/1212 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2010 Revista Fitos Eletrônica info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2010 Revista Fitos Eletrônica |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
dc.source.none.fl_str_mv |
Revista Fitos; Vol. 5 No. 01 (2010); 92-98 Revista Fitos; Vol. 5 Núm. 01 (2010); 92-98 Revista Fitos; v. 5 n. 01 (2010); 92-98 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Revista Fitos |
collection |
Revista Fitos |
repository.name.fl_str_mv |
Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
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1798313473685323776 |