In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Detalhes bibliográficos
Autor(a) principal: Victorio, Cristiane Pimentel
Data de Publicação: 2021
Outros Autores: Silva, Davi Oliveira e, Alviano, Daniela, Alviano, Celuta, Kuster, Ricardo Machado, Lage, Celso Luiz Salgueiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Fitos
Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037
Resumo: This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.
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spelling In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extractAtividade antimicrobiana in vitro das frações apolares dos extratos de folhas de Alpinia zerumbet e A. purpurataAntifungal activityZingiberaceaeAgar drop diffusionFilamentous fungiYeastAlpiniaAtividade antifúngicaZingiberaceaeDifusão em agarFungos filamentososLevedurasThis study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.Este trabalho teve como objetivo avaliar a atividade antimicrobiana in vitro das frações dos extratos brutos hidroalcoólicos de folhas: hexano, diclorometano, acetato de etila e butanólico de Alpinia zerumbet e A. purpurata pelo método de difusão em ágar gota a fim de triar os principais compostos envolvidos na atividade antimicrobiana. Folhas de plantas adultas de Alpinia zerumbet (Pers.) B.L. Burtt et R.M. Sm. e A. purpurata (Vieill) K. Schum foram coletadas secas e maceradas em etanol a 70%. Os extratos etanólicos foram particionados com solventes de polaridade crescente. As frações de diclorometano foram analisadas por cromatografia gasosa/espectrometria de massa (CG/ EM). A principal substância na fração diclorometânica de ambas as espécies foi o ácido hexadecanóico (ácido palmítico). A atividade antimicrobiana do extrato bruto, bem como das frações de hexano, diclorometano, acetato de etila e butanol foram avaliadas contra bactérias e fungos patogênicos utilizando o teste de difusão em agar. Não houve inibição das bactérias testadas. Entretanto, a fração diclorometânica apresentou atividade antifúngica promissora contra os seguintes fungos: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis e M. gypseum.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2021-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmltext/xmltext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/103710.32712/2446-4775.2021.1037Revista Fitos; Vol. 15 No. 2 (2021); 136-143Revista Fitos; Vol. 15 Núm. 2 (2021); 136-143Revista Fitos; v. 15 n. 2 (2021); 136-1432446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/775https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/776https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/852https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/855Copyright (c) 2019 Revista Fitoshttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessVictorio, Cristiane PimentelSilva, Davi Oliveira eAlviano, DanielaAlviano, Celuta Kuster, Ricardo MachadoLage, Celso Luiz Salgueiro2021-07-01T20:46:52Zoai:ojs.revistafitos.far.fiocruz.br:article/1037Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2021-07-01T20:46:52Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
Atividade antimicrobiana in vitro das frações apolares dos extratos de folhas de Alpinia zerumbet e A. purpurata
title In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
spellingShingle In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
Victorio, Cristiane Pimentel
Antifungal activity
Zingiberaceae
Agar drop diffusion
Filamentous fungi
Yeast
Alpinia
Atividade antifúngica
Zingiberaceae
Difusão em agar
Fungos filamentosos
Leveduras
title_short In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
title_full In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
title_fullStr In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
title_full_unstemmed In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
title_sort In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
author Victorio, Cristiane Pimentel
author_facet Victorio, Cristiane Pimentel
Silva, Davi Oliveira e
Alviano, Daniela
Alviano, Celuta
Kuster, Ricardo Machado
Lage, Celso Luiz Salgueiro
author_role author
author2 Silva, Davi Oliveira e
Alviano, Daniela
Alviano, Celuta
Kuster, Ricardo Machado
Lage, Celso Luiz Salgueiro
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Victorio, Cristiane Pimentel
Silva, Davi Oliveira e
Alviano, Daniela
Alviano, Celuta
Kuster, Ricardo Machado
Lage, Celso Luiz Salgueiro
dc.subject.por.fl_str_mv Antifungal activity
Zingiberaceae
Agar drop diffusion
Filamentous fungi
Yeast
Alpinia
Atividade antifúngica
Zingiberaceae
Difusão em agar
Fungos filamentosos
Leveduras
topic Antifungal activity
Zingiberaceae
Agar drop diffusion
Filamentous fungi
Yeast
Alpinia
Atividade antifúngica
Zingiberaceae
Difusão em agar
Fungos filamentosos
Leveduras
description This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037
10.32712/2446-4775.2021.1037
url https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037
identifier_str_mv 10.32712/2446-4775.2021.1037
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/775
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/776
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/852
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1037/855
dc.rights.driver.fl_str_mv Copyright (c) 2019 Revista Fitos
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Revista Fitos
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
text/xml
text/html
dc.publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
dc.source.none.fl_str_mv Revista Fitos; Vol. 15 No. 2 (2021); 136-143
Revista Fitos; Vol. 15 Núm. 2 (2021); 136-143
Revista Fitos; v. 15 n. 2 (2021); 136-143
2446-4775
1808-9569
reponame:Revista Fitos
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Revista Fitos
collection Revista Fitos
repository.name.fl_str_mv Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br
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