Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)

Detalhes bibliográficos
Autor(a) principal: Da Silva, Weison Lima
Data de Publicação: 2020
Outros Autores: Fachin-Espinar, María Teresa, de Souza, Maria Carolina Scheffer, Lima, Laísley Martins, Nunez, Cecilia Veronica
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Fitos
Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891
Resumo: The present work investigated the biotechnological potential of the endophytic fungus Graphium jumulu. The fungus was grown in potato broth supplemented with 0.2% yeast extract in a shaker incubator at 120 rpm at 30ºC for 20 days, the metabolites of the mycelium being extracted with ethyl acetate in an ultrasound bath. The extract was tested for toxicity, antioxidant and antibacterial activities. The extract did not show a toxic and antioxidant effect, showing only antibacterial activity, which led to its fractionation and subsequent isolation of the substance: 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone. This was tested against the pathogenic strains Staphylococcus aureus, Salmonella enteritidis, Aeromonas hydrophila, Pseudomonas fluorescens, Klebsiella pneumoniae, Edwardsiella tarda, Acinetobacter baumannii, Serratia marcencens, Pseudomonas aeruginosa, Escherichia coli. The substance did not inhibit any of the strains evaluated, but stimulated bacterial growth of all, with emphasis on S. aureus, A. baumannii and S. enteritidis, which presented a linear growth as function of the substance’s concentration. It is concluded that 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone is a potential microbial growth stimulant suggesting the continuity of evaluations against other bacteria of clinical interest and of industrial interest. This is the first report of this substance in the endophytic fungus Graphium jumulu.
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spelling Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)Avaliação química e biológica de Graphium jumulu, fungo endofítico de Duroia macrophylla Huber (Rubiaceae)BioprospectionGraphium jumuluAntibacterial activityPerylene quinoneBioprospecçãoGraphium jumuluAtividade antibacterianaPerileno quinonaThe present work investigated the biotechnological potential of the endophytic fungus Graphium jumulu. The fungus was grown in potato broth supplemented with 0.2% yeast extract in a shaker incubator at 120 rpm at 30ºC for 20 days, the metabolites of the mycelium being extracted with ethyl acetate in an ultrasound bath. The extract was tested for toxicity, antioxidant and antibacterial activities. The extract did not show a toxic and antioxidant effect, showing only antibacterial activity, which led to its fractionation and subsequent isolation of the substance: 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone. This was tested against the pathogenic strains Staphylococcus aureus, Salmonella enteritidis, Aeromonas hydrophila, Pseudomonas fluorescens, Klebsiella pneumoniae, Edwardsiella tarda, Acinetobacter baumannii, Serratia marcencens, Pseudomonas aeruginosa, Escherichia coli. The substance did not inhibit any of the strains evaluated, but stimulated bacterial growth of all, with emphasis on S. aureus, A. baumannii and S. enteritidis, which presented a linear growth as function of the substance’s concentration. It is concluded that 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone is a potential microbial growth stimulant suggesting the continuity of evaluations against other bacteria of clinical interest and of industrial interest. This is the first report of this substance in the endophytic fungus Graphium jumulu.O presente trabalho investigou o potencial biotecnológico do fungo endofítico Graphium jumulu. O fungo foi cultivado em meio caldo de batata suplementado com 0,2% de extrato de levedura, em agitação a 120 rpm, a 30°C durante 20 dias, sendo os metabólitos do micélio extraídos com acetato de etila em banho ultrassom. O extrato foi testado para as atividades de toxicidade, antioxidante e antibacteriana, e não demonstrou efeito tóxico e nem atividade antioxidante, apresentando somente atividade antibacteriana, o que levou a seu fracionamento e, posterior, isolamento da substância: 4,9-dihidroxi-1,2,11,12-tetrahidroperileno-3,10-quinona. Esta substância foi testada frente as cepas patogênicas Acinetobacter baumannii, Aeromonas hydrophila, Edwardsiella tarda, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella enteritidis, Serratia marcencens, Staphylococcus aureus. A substância não inibiu nenhuma das cepas avaliadas, entretanto estimulou o crescimento bacteriano de todas, com destaque para S. aureus, A. baumannii e S. enteritidis, que apresentaram um crescimento de forma linear crescente em função da concentração da substância. Conclui-se que a 4,9-dihidroxi-1, 2,11,12-tetrahidroperileno-3,10-quinona é um potencial estimulador do crescimento microbiano sugerindo a continuidade de avaliações frente a outras bactérias de interesse clínico e de interesse industrial. Este é o primeiro relato desta substância no fungo endofítico G. jumulu.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2020-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/89110.32712/2446-4775.2020.891Revista Fitos; Vol. 14 No. 2 (2020); 238-248Revista Fitos; Vol. 14 Núm. 2 (2020); 238-248Revista Fitos; v. 14 n. 2 (2020); 238-2482446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/701https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/702Copyright (c) 2020 Revista Fitoshttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessDa Silva, Weison LimaFachin-Espinar, María Teresade Souza, Maria Carolina SchefferLima, Laísley MartinsNunez, Cecilia Veronica2020-09-30T14:59:12Zoai:ojs.revistafitos.far.fiocruz.br:article/891Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2020-09-30T14:59:12Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
Avaliação química e biológica de Graphium jumulu, fungo endofítico de Duroia macrophylla Huber (Rubiaceae)
title Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
spellingShingle Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
Da Silva, Weison Lima
Bioprospection
Graphium jumulu
Antibacterial activity
Perylene quinone
Bioprospecção
Graphium jumulu
Atividade antibacteriana
Perileno quinona
title_short Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
title_full Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
title_fullStr Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
title_full_unstemmed Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
title_sort Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
author Da Silva, Weison Lima
author_facet Da Silva, Weison Lima
Fachin-Espinar, María Teresa
de Souza, Maria Carolina Scheffer
Lima, Laísley Martins
Nunez, Cecilia Veronica
author_role author
author2 Fachin-Espinar, María Teresa
de Souza, Maria Carolina Scheffer
Lima, Laísley Martins
Nunez, Cecilia Veronica
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Da Silva, Weison Lima
Fachin-Espinar, María Teresa
de Souza, Maria Carolina Scheffer
Lima, Laísley Martins
Nunez, Cecilia Veronica
dc.subject.por.fl_str_mv Bioprospection
Graphium jumulu
Antibacterial activity
Perylene quinone
Bioprospecção
Graphium jumulu
Atividade antibacteriana
Perileno quinona
topic Bioprospection
Graphium jumulu
Antibacterial activity
Perylene quinone
Bioprospecção
Graphium jumulu
Atividade antibacteriana
Perileno quinona
description The present work investigated the biotechnological potential of the endophytic fungus Graphium jumulu. The fungus was grown in potato broth supplemented with 0.2% yeast extract in a shaker incubator at 120 rpm at 30ºC for 20 days, the metabolites of the mycelium being extracted with ethyl acetate in an ultrasound bath. The extract was tested for toxicity, antioxidant and antibacterial activities. The extract did not show a toxic and antioxidant effect, showing only antibacterial activity, which led to its fractionation and subsequent isolation of the substance: 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone. This was tested against the pathogenic strains Staphylococcus aureus, Salmonella enteritidis, Aeromonas hydrophila, Pseudomonas fluorescens, Klebsiella pneumoniae, Edwardsiella tarda, Acinetobacter baumannii, Serratia marcencens, Pseudomonas aeruginosa, Escherichia coli. The substance did not inhibit any of the strains evaluated, but stimulated bacterial growth of all, with emphasis on S. aureus, A. baumannii and S. enteritidis, which presented a linear growth as function of the substance’s concentration. It is concluded that 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone is a potential microbial growth stimulant suggesting the continuity of evaluations against other bacteria of clinical interest and of industrial interest. This is the first report of this substance in the endophytic fungus Graphium jumulu.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891
10.32712/2446-4775.2020.891
url https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891
identifier_str_mv 10.32712/2446-4775.2020.891
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/701
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/702
dc.rights.driver.fl_str_mv Copyright (c) 2020 Revista Fitos
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Revista Fitos
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
dc.source.none.fl_str_mv Revista Fitos; Vol. 14 No. 2 (2020); 238-248
Revista Fitos; Vol. 14 Núm. 2 (2020); 238-248
Revista Fitos; v. 14 n. 2 (2020); 238-248
2446-4775
1808-9569
reponame:Revista Fitos
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Revista Fitos
collection Revista Fitos
repository.name.fl_str_mv Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br
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