Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Fitos |
Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891 |
Resumo: | The present work investigated the biotechnological potential of the endophytic fungus Graphium jumulu. The fungus was grown in potato broth supplemented with 0.2% yeast extract in a shaker incubator at 120 rpm at 30ºC for 20 days, the metabolites of the mycelium being extracted with ethyl acetate in an ultrasound bath. The extract was tested for toxicity, antioxidant and antibacterial activities. The extract did not show a toxic and antioxidant effect, showing only antibacterial activity, which led to its fractionation and subsequent isolation of the substance: 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone. This was tested against the pathogenic strains Staphylococcus aureus, Salmonella enteritidis, Aeromonas hydrophila, Pseudomonas fluorescens, Klebsiella pneumoniae, Edwardsiella tarda, Acinetobacter baumannii, Serratia marcencens, Pseudomonas aeruginosa, Escherichia coli. The substance did not inhibit any of the strains evaluated, but stimulated bacterial growth of all, with emphasis on S. aureus, A. baumannii and S. enteritidis, which presented a linear growth as function of the substance’s concentration. It is concluded that 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone is a potential microbial growth stimulant suggesting the continuity of evaluations against other bacteria of clinical interest and of industrial interest. This is the first report of this substance in the endophytic fungus Graphium jumulu. |
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Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae)Avaliação química e biológica de Graphium jumulu, fungo endofítico de Duroia macrophylla Huber (Rubiaceae)BioprospectionGraphium jumuluAntibacterial activityPerylene quinoneBioprospecçãoGraphium jumuluAtividade antibacterianaPerileno quinonaThe present work investigated the biotechnological potential of the endophytic fungus Graphium jumulu. The fungus was grown in potato broth supplemented with 0.2% yeast extract in a shaker incubator at 120 rpm at 30ºC for 20 days, the metabolites of the mycelium being extracted with ethyl acetate in an ultrasound bath. The extract was tested for toxicity, antioxidant and antibacterial activities. The extract did not show a toxic and antioxidant effect, showing only antibacterial activity, which led to its fractionation and subsequent isolation of the substance: 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone. This was tested against the pathogenic strains Staphylococcus aureus, Salmonella enteritidis, Aeromonas hydrophila, Pseudomonas fluorescens, Klebsiella pneumoniae, Edwardsiella tarda, Acinetobacter baumannii, Serratia marcencens, Pseudomonas aeruginosa, Escherichia coli. The substance did not inhibit any of the strains evaluated, but stimulated bacterial growth of all, with emphasis on S. aureus, A. baumannii and S. enteritidis, which presented a linear growth as function of the substance’s concentration. It is concluded that 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone is a potential microbial growth stimulant suggesting the continuity of evaluations against other bacteria of clinical interest and of industrial interest. This is the first report of this substance in the endophytic fungus Graphium jumulu.O presente trabalho investigou o potencial biotecnológico do fungo endofítico Graphium jumulu. O fungo foi cultivado em meio caldo de batata suplementado com 0,2% de extrato de levedura, em agitação a 120 rpm, a 30°C durante 20 dias, sendo os metabólitos do micélio extraídos com acetato de etila em banho ultrassom. O extrato foi testado para as atividades de toxicidade, antioxidante e antibacteriana, e não demonstrou efeito tóxico e nem atividade antioxidante, apresentando somente atividade antibacteriana, o que levou a seu fracionamento e, posterior, isolamento da substância: 4,9-dihidroxi-1,2,11,12-tetrahidroperileno-3,10-quinona. Esta substância foi testada frente as cepas patogênicas Acinetobacter baumannii, Aeromonas hydrophila, Edwardsiella tarda, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella enteritidis, Serratia marcencens, Staphylococcus aureus. A substância não inibiu nenhuma das cepas avaliadas, entretanto estimulou o crescimento bacteriano de todas, com destaque para S. aureus, A. baumannii e S. enteritidis, que apresentaram um crescimento de forma linear crescente em função da concentração da substância. Conclui-se que a 4,9-dihidroxi-1, 2,11,12-tetrahidroperileno-3,10-quinona é um potencial estimulador do crescimento microbiano sugerindo a continuidade de avaliações frente a outras bactérias de interesse clínico e de interesse industrial. Este é o primeiro relato desta substância no fungo endofítico G. jumulu.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2020-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/89110.32712/2446-4775.2020.891Revista Fitos; Vol. 14 No. 2 (2020); 238-248Revista Fitos; Vol. 14 Núm. 2 (2020); 238-248Revista Fitos; v. 14 n. 2 (2020); 238-2482446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/701https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/702Copyright (c) 2020 Revista Fitoshttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessDa Silva, Weison LimaFachin-Espinar, María Teresade Souza, Maria Carolina SchefferLima, Laísley MartinsNunez, Cecilia Veronica2020-09-30T14:59:12Zoai:ojs.revistafitos.far.fiocruz.br:article/891Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2020-09-30T14:59:12Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) Avaliação química e biológica de Graphium jumulu, fungo endofítico de Duroia macrophylla Huber (Rubiaceae) |
title |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) |
spellingShingle |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) Da Silva, Weison Lima Bioprospection Graphium jumulu Antibacterial activity Perylene quinone Bioprospecção Graphium jumulu Atividade antibacteriana Perileno quinona |
title_short |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) |
title_full |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) |
title_fullStr |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) |
title_full_unstemmed |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) |
title_sort |
Chemical and biological evaluation of Graphium jumulu, endophytic fungus of Duroia macrophylla Huber (Rubiaceae) |
author |
Da Silva, Weison Lima |
author_facet |
Da Silva, Weison Lima Fachin-Espinar, María Teresa de Souza, Maria Carolina Scheffer Lima, Laísley Martins Nunez, Cecilia Veronica |
author_role |
author |
author2 |
Fachin-Espinar, María Teresa de Souza, Maria Carolina Scheffer Lima, Laísley Martins Nunez, Cecilia Veronica |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Da Silva, Weison Lima Fachin-Espinar, María Teresa de Souza, Maria Carolina Scheffer Lima, Laísley Martins Nunez, Cecilia Veronica |
dc.subject.por.fl_str_mv |
Bioprospection Graphium jumulu Antibacterial activity Perylene quinone Bioprospecção Graphium jumulu Atividade antibacteriana Perileno quinona |
topic |
Bioprospection Graphium jumulu Antibacterial activity Perylene quinone Bioprospecção Graphium jumulu Atividade antibacteriana Perileno quinona |
description |
The present work investigated the biotechnological potential of the endophytic fungus Graphium jumulu. The fungus was grown in potato broth supplemented with 0.2% yeast extract in a shaker incubator at 120 rpm at 30ºC for 20 days, the metabolites of the mycelium being extracted with ethyl acetate in an ultrasound bath. The extract was tested for toxicity, antioxidant and antibacterial activities. The extract did not show a toxic and antioxidant effect, showing only antibacterial activity, which led to its fractionation and subsequent isolation of the substance: 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone. This was tested against the pathogenic strains Staphylococcus aureus, Salmonella enteritidis, Aeromonas hydrophila, Pseudomonas fluorescens, Klebsiella pneumoniae, Edwardsiella tarda, Acinetobacter baumannii, Serratia marcencens, Pseudomonas aeruginosa, Escherichia coli. The substance did not inhibit any of the strains evaluated, but stimulated bacterial growth of all, with emphasis on S. aureus, A. baumannii and S. enteritidis, which presented a linear growth as function of the substance’s concentration. It is concluded that 4,9-dihydroxy-1,2,11,12-tetrahydroperylene-3,10-quinone is a potential microbial growth stimulant suggesting the continuity of evaluations against other bacteria of clinical interest and of industrial interest. This is the first report of this substance in the endophytic fungus Graphium jumulu. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891 10.32712/2446-4775.2020.891 |
url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891 |
identifier_str_mv |
10.32712/2446-4775.2020.891 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/701 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/891/702 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Revista Fitos https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Revista Fitos https://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
dc.source.none.fl_str_mv |
Revista Fitos; Vol. 14 No. 2 (2020); 238-248 Revista Fitos; Vol. 14 Núm. 2 (2020); 238-248 Revista Fitos; v. 14 n. 2 (2020); 238-248 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Revista Fitos |
collection |
Revista Fitos |
repository.name.fl_str_mv |
Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
_version_ |
1798313475067346944 |