Evaluation of the genotoxicity of aqueous extracts of Moringa oleifera Lam. (Moringaceae) leaves using the Allium cepa test

• Autor(a) principal: Altamirano, Carlos Gustavo
• Data de Publicação: 2020
• Outros Autores: Aguilar Pozzer, Maria Jose, Rebatta, Jose Luis, Semckzuk, Rosaura Ines, Florentín, Alejandro Paul, Sandoval Fernandez, Maria de los Angeles
• Tipo de documento: Artigo
• Idioma: spa
• Título da fonte: Revista Fitos
• Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/809
• topic: Genotoxicity, M. oleifer. Aqueous extracts. A. cepa test.; genotoxicidad; M. oleifera; extractos acuosos; A. cepa test

Currently the current use of medicinal plants for the treatment of different conditions has increased and in most cases they are commercialized without previous toxicity studies. M. oleifera is a tree distributed in tropical and subtropical zones, widely used in traditional medicine to treat multiple conditions. Within the framework of the Research Project incentivized 16Q626 “Evaluación de la Genotoxicidad de Extractivos de Especies Medicinales de uso corriente para las afecciones más frecuentes en la Ciudad de Posadas Provincia de Misiones Argentina”, in this instance it was proposed to analyze the genotoxicity of aqueous extracts of M. oleifera by means of the Allium cepa test. The experiment consisted of two stages, where in addition to determining the concentration that stops the growth of the roots by 50% in comparison with the controls and the macroscopic anomalies, the predetermined biomarkers of genotoxicity (lagged chromosomes, c-mitoses, anaphase bridge, etc.) at concentrations equal to or greater than the concentration 50. The highest concentrations tested showed greater inhibition of mitosis with respect to the controls, but no genotoxic effects were found, although the doses used in the experiment are not usual doses of M. oleifera in traditional medicine.