Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Fitos |
Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616 |
Resumo: | Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) is a medicinal plant commonly known as ipecac, which contains two alkaloids of high pharmacological value (emetine and cephaeline) with anti-diarrheal, amebicidal, expectorant and anti-inflammatory properties. The risk of extinction and the difficulty of the conventional cultivation of the species combined with great market demand has generated a need for studies of cultivation methods that allow the economic exploitation of this species. This work presents data on the development of an in vitro root culture protocol for P. ipecacuanha. Leaf, nodal, internodal and root segments were used, which were introduced in culture media containing different concentrations (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg.L-1) of indolbutiric acid. The best treatment for root formation included the use of nodal segments in culture media containing 2.5 and 3.0 mg.L-1 of IBA, which gave rise to 100% root formation at 60 days of culture. Regarding the number of roots per explant, the best result (13:1) was obtained with the use of internodal segments in medium supplemented with 2.5 mg.L-1 IBA and, the best root length was obtained in root segments developed in medium enriched with 1.5 mg.L-1 IBA, with an average increment of 0.87 cm per root. |
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Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae)Efeito do ácido indolbutírico na produção de raízes in vitro de Psychotria ipecacuanha (Brot.) Stokes – RubiaceaeIpecac. Root culture. In vitro culture. Plant biotechnology.Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) is a medicinal plant commonly known as ipecac, which contains two alkaloids of high pharmacological value (emetine and cephaeline) with anti-diarrheal, amebicidal, expectorant and anti-inflammatory properties. The risk of extinction and the difficulty of the conventional cultivation of the species combined with great market demand has generated a need for studies of cultivation methods that allow the economic exploitation of this species. This work presents data on the development of an in vitro root culture protocol for P. ipecacuanha. Leaf, nodal, internodal and root segments were used, which were introduced in culture media containing different concentrations (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg.L-1) of indolbutiric acid. The best treatment for root formation included the use of nodal segments in culture media containing 2.5 and 3.0 mg.L-1 of IBA, which gave rise to 100% root formation at 60 days of culture. Regarding the number of roots per explant, the best result (13:1) was obtained with the use of internodal segments in medium supplemented with 2.5 mg.L-1 IBA and, the best root length was obtained in root segments developed in medium enriched with 1.5 mg.L-1 IBA, with an average increment of 0.87 cm per root.Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) é uma planta medicinal, conhecida como ipeca, que em suas raízes, são encontrados dois alcalóides de grande valor farmacológico (emetina e cefalina), usados no tratamento antidiarréico, amebicida, expectorante e antiinflamatório. O risco de extinção e a dificuldade do cultivo convencional da espécie, frente à grande demanda de mercado, fomentam estudos de métodos de cultivo que possibilitem a exploração econômica da mesma. Este trabalho apresenta dados sobre o desenvolvimento de protocolo de cultivo de raízes in vitro de P. ipecacuanha. Foram utilizadas folhas, segmentos nodais, internodais e raízes, que foram introduzidos em meios de cultura contendo diferentes concentrações (0,5; 1,0; 1,5; 2,0; 2,5 e 3,0 mg.L-1) de ácido indolbutírico. O melhor tratamento para a formação de raízes foi com a utilização de segmentos nodais, em meio de cultura contendo 2,5 e 3,0 mg.L-1 de AIB, que deram origem a 100% de formação de raízes, aos 60 dias de cultivo. Com relação ao número de raízes, o melhor resultado (13:1), foi obtido com a utilização de segmentos internodais em meio suplementado com 2,5 mg.L-1 de AIB e, o melhor comprimento de raízes foi obtido em segmentos radiculares desenvolvidos em 1,5 mg.L-1 de AIB (0,87cm por raiz).Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2018-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/61610.17648/2446-4775.2018.616Revista Fitos; Vol. 12 No. 3 (2018); 218-226Revista Fitos; Vol. 12 Núm. 3 (2018); 218-226Revista Fitos; v. 12 n. 3 (2018); 218-2262446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616/pdfhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616/htmlCopyright (c) 2018 Revista Fitos Eletrônicainfo:eu-repo/semantics/openAccessda Silva, SimoneAstolfi Filho, Spartaco2021-01-29T13:22:32Zoai:ojs.revistafitos.far.fiocruz.br:article/616Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2021-01-29T13:22:32Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) Efeito do ácido indolbutírico na produção de raízes in vitro de Psychotria ipecacuanha (Brot.) Stokes – Rubiaceae |
title |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) |
spellingShingle |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) da Silva, Simone Ipecac. Root culture. In vitro culture. Plant biotechnology. |
title_short |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) |
title_full |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) |
title_fullStr |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) |
title_full_unstemmed |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) |
title_sort |
Effect of indolebutyric acid on in vitro root production of Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) |
author |
da Silva, Simone |
author_facet |
da Silva, Simone Astolfi Filho, Spartaco |
author_role |
author |
author2 |
Astolfi Filho, Spartaco |
author2_role |
author |
dc.contributor.author.fl_str_mv |
da Silva, Simone Astolfi Filho, Spartaco |
dc.subject.por.fl_str_mv |
Ipecac. Root culture. In vitro culture. Plant biotechnology. |
topic |
Ipecac. Root culture. In vitro culture. Plant biotechnology. |
description |
Psychotria ipecacuanha (Brot.) Stokes (Rubiaceae) is a medicinal plant commonly known as ipecac, which contains two alkaloids of high pharmacological value (emetine and cephaeline) with anti-diarrheal, amebicidal, expectorant and anti-inflammatory properties. The risk of extinction and the difficulty of the conventional cultivation of the species combined with great market demand has generated a need for studies of cultivation methods that allow the economic exploitation of this species. This work presents data on the development of an in vitro root culture protocol for P. ipecacuanha. Leaf, nodal, internodal and root segments were used, which were introduced in culture media containing different concentrations (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg.L-1) of indolbutiric acid. The best treatment for root formation included the use of nodal segments in culture media containing 2.5 and 3.0 mg.L-1 of IBA, which gave rise to 100% root formation at 60 days of culture. Regarding the number of roots per explant, the best result (13:1) was obtained with the use of internodal segments in medium supplemented with 2.5 mg.L-1 IBA and, the best root length was obtained in root segments developed in medium enriched with 1.5 mg.L-1 IBA, with an average increment of 0.87 cm per root. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616 10.17648/2446-4775.2018.616 |
url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616 |
identifier_str_mv |
10.17648/2446-4775.2018.616 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616/pdf https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/616/html |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Revista Fitos Eletrônica info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Revista Fitos Eletrônica |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf text/html |
dc.publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
publisher.none.fl_str_mv |
Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
dc.source.none.fl_str_mv |
Revista Fitos; Vol. 12 No. 3 (2018); 218-226 Revista Fitos; Vol. 12 Núm. 3 (2018); 218-226 Revista Fitos; v. 12 n. 3 (2018); 218-226 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Revista Fitos |
collection |
Revista Fitos |
repository.name.fl_str_mv |
Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
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1798313474720268288 |