Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Pica-Mattoccia,Livia
Data de Publicação: 1992
Outros Autores: Dias,Luiz Candido de Souza, Cioli,Donato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800032
Resumo: The objective of this study is to determine whether various hycanthone resistant strains of schistosomes which have been independently isolated are all affected in the same gene. A strain obtained from a Brazilian patient was compared with a strain of Puerto Rican origin selected in the laboratory. If the mutation conferring resistance involved two different genes, one would expect that the progeny of a cross between the two strains would show complementation, i.e. it would be sensitive to the drug. We have performed such a cross and obtained F1 hybrid worms wich were essentially all resistant, thus suggesting that the mutation conferring resistance in the two strains involves the same gene.
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spelling Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoniSchistosoma mansonihycanthone resistancegenetic complementationThe objective of this study is to determine whether various hycanthone resistant strains of schistosomes which have been independently isolated are all affected in the same gene. A strain obtained from a Brazilian patient was compared with a strain of Puerto Rican origin selected in the laboratory. If the mutation conferring resistance involved two different genes, one would expect that the progeny of a cross between the two strains would show complementation, i.e. it would be sensitive to the drug. We have performed such a cross and obtained F1 hybrid worms wich were essentially all resistant, thus suggesting that the mutation conferring resistance in the two strains involves the same gene.Instituto Oswaldo Cruz, Ministério da Saúde1992-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800032Memórias do Instituto Oswaldo Cruz v.87 suppl.4 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000800032info:eu-repo/semantics/openAccessPica-Mattoccia,LiviaDias,Luiz Candido de SouzaCioli,Donatoeng2020-04-25T17:47:02Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:05:21.097Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
title Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
spellingShingle Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
Pica-Mattoccia,Livia
Schistosoma mansoni
hycanthone resistance
genetic complementation
title_short Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
title_full Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
title_fullStr Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
title_full_unstemmed Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
title_sort Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni
author Pica-Mattoccia,Livia
author_facet Pica-Mattoccia,Livia
Dias,Luiz Candido de Souza
Cioli,Donato
author_role author
author2 Dias,Luiz Candido de Souza
Cioli,Donato
author2_role author
author
dc.contributor.author.fl_str_mv Pica-Mattoccia,Livia
Dias,Luiz Candido de Souza
Cioli,Donato
dc.subject.por.fl_str_mv Schistosoma mansoni
hycanthone resistance
genetic complementation
topic Schistosoma mansoni
hycanthone resistance
genetic complementation
dc.description.none.fl_txt_mv The objective of this study is to determine whether various hycanthone resistant strains of schistosomes which have been independently isolated are all affected in the same gene. A strain obtained from a Brazilian patient was compared with a strain of Puerto Rican origin selected in the laboratory. If the mutation conferring resistance involved two different genes, one would expect that the progeny of a cross between the two strains would show complementation, i.e. it would be sensitive to the drug. We have performed such a cross and obtained F1 hybrid worms wich were essentially all resistant, thus suggesting that the mutation conferring resistance in the two strains involves the same gene.
description The objective of this study is to determine whether various hycanthone resistant strains of schistosomes which have been independently isolated are all affected in the same gene. A strain obtained from a Brazilian patient was compared with a strain of Puerto Rican origin selected in the laboratory. If the mutation conferring resistance involved two different genes, one would expect that the progeny of a cross between the two strains would show complementation, i.e. it would be sensitive to the drug. We have performed such a cross and obtained F1 hybrid worms wich were essentially all resistant, thus suggesting that the mutation conferring resistance in the two strains involves the same gene.
publishDate 1992
dc.date.none.fl_str_mv 1992-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800032
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000800032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000800032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 suppl.4 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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