Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Batista Júnior,João
Data de Publicação: 2010
Outros Autores: Berzaghi,Rodrigo, Arnaud,Adelita Duanra Medeiros do Monte, Fontes,Cor Jesus Fernandes, Camargo,Zoilo Pires de, Hahn,Rosane Christine
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100009
Resumo: This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.
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spelling Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensisParacoccidioides brasiliensissimultaneous infectionRAPDThis study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.Instituto Oswaldo Cruz, Ministério da Saúde2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100009Memórias do Instituto Oswaldo Cruz v.105 n.1 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000100009info:eu-repo/semantics/openAccessBatista Júnior,JoãoBerzaghi,RodrigoArnaud,Adelita Duanra Medeiros do MonteFontes,Cor Jesus FernandesCamargo,Zoilo Pires deHahn,Rosane Christineeng2020-04-25T17:50:38Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:46.239Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
title Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
spellingShingle Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
Batista Júnior,João
Paracoccidioides brasiliensis
simultaneous infection
RAPD
title_short Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
title_full Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
title_fullStr Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
title_full_unstemmed Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
title_sort Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis
author Batista Júnior,João
author_facet Batista Júnior,João
Berzaghi,Rodrigo
Arnaud,Adelita Duanra Medeiros do Monte
Fontes,Cor Jesus Fernandes
Camargo,Zoilo Pires de
Hahn,Rosane Christine
author_role author
author2 Berzaghi,Rodrigo
Arnaud,Adelita Duanra Medeiros do Monte
Fontes,Cor Jesus Fernandes
Camargo,Zoilo Pires de
Hahn,Rosane Christine
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Batista Júnior,João
Berzaghi,Rodrigo
Arnaud,Adelita Duanra Medeiros do Monte
Fontes,Cor Jesus Fernandes
Camargo,Zoilo Pires de
Hahn,Rosane Christine
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
simultaneous infection
RAPD
topic Paracoccidioides brasiliensis
simultaneous infection
RAPD
dc.description.none.fl_txt_mv This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.
description This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe a simultaneous infection with genetically distinct isolates of P. brasiliensis in a patient with chronic paracoccidioidomycosis. The clinical isolates were obtained from lesions in different anatomical sites and were characterised by random amplified polymorphic DNA (RAPD) analysis. The RAPD technique can be helpful for distinguishing between clinical isolates. Different random primers were used to characterise these clinical isolates. The RAPD patterns allowed for differentiation between isolates and the construction of a phenetic tree, which showed more than 28% genetic variability in this fungal species, opening new possibilities for clinical studies of P. brasiliensis. Based on these results and preliminary clinical findings, we suggest that different genotypes of P. brasiliensis might infect the same patient, inducing the active form of the disease.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.1 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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