Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns

Detalhes bibliográficos
Autor(a) principal: Kurokawa, Cilmery S.
Data de Publicação: 2005
Outros Autores: Lopes, Catalina R., Sugizaki, Maria F., Kuramae, Eiko E., Franco, Marcello F., Peraçoli, Maria T. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30935
Resumo: Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast cell recovery from lungs and demonstration of histopathologic lesions in different organs. The results showed that the isolates presented four virulence degrees: high virulence, intermediate, low and non-virulence. RAPD clustered the isolates studied in two main groups with 56% of genetic similarity. Strains with low virulence, Pb265 or the non-virulent, Pb192, showed glabrous/cerebriform morphology and high genetic similarity (98.7%) when compared to the other isolates studied. The same was observed with Bt79 and Bt83 that shared 96% genetic similarity, cottony colonies and high virulence. The RAPD technique could only discriminate P. brasiliensis isolates according to glabrous/cerebriform or cottony colonies, and also high from low virulence strains. Isolates with intermediate virulence such as Pb18, Pb18B6, Bt32 and Bt56 showed variability in their similarity coefficient suggesting that RAPD was able to detect genetic variability in this fungal specie. Virulence profile of P. brasiliensis demonstrated that both mycelial morphologic extreme phenotypes may be associated with fungal virulence and their in vitro subculture time. Thus, RAPD technique analysis employed in association with virulence, morphologic and immunologic aspects might prove adequate to detect differences between P. brasiliensis isolates.
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spelling Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético AntibodiesColony morphologyParacoccidioides brasiliensisRAPDVirulence Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast cell recovery from lungs and demonstration of histopathologic lesions in different organs. The results showed that the isolates presented four virulence degrees: high virulence, intermediate, low and non-virulence. RAPD clustered the isolates studied in two main groups with 56% of genetic similarity. Strains with low virulence, Pb265 or the non-virulent, Pb192, showed glabrous/cerebriform morphology and high genetic similarity (98.7%) when compared to the other isolates studied. The same was observed with Bt79 and Bt83 that shared 96% genetic similarity, cottony colonies and high virulence. The RAPD technique could only discriminate P. brasiliensis isolates according to glabrous/cerebriform or cottony colonies, and also high from low virulence strains. Isolates with intermediate virulence such as Pb18, Pb18B6, Bt32 and Bt56 showed variability in their similarity coefficient suggesting that RAPD was able to detect genetic variability in this fungal specie. Virulence profile of P. brasiliensis demonstrated that both mycelial morphologic extreme phenotypes may be associated with fungal virulence and their in vitro subculture time. Thus, RAPD technique analysis employed in association with virulence, morphologic and immunologic aspects might prove adequate to detect differences between P. brasiliensis isolates. Dez isolados de P. brasiliensis foram avaliados em relação à patogenicidade por inoculação intravenosa em camundongos e associação com morfologia miceliana e padrão genético por amplificação genônica do DNA polimórfico (RAPD). A patogenicidade, avaliada por recuperação de fungos viáveis a partir de tecido pulmonar e por lesões histopatológicas em diferentes órgãos, mostrou que os isolados apresentaram quatro graus de virulência: alta virulência, virulência intermediária, baixa virulência e não virulência. A técnica de RAPD agrupou os isolados em dois grupos com 56% de similaridade genética. Amostras com baixa virulência Pb265 ou não virulência Pb192 apresentaram morfologia glabra/cerebriforme e alta similaridade genética (98,7%) quando comparadas com os outros isolados estudados. O mesmo foi observado com os isolados Bt79 e Bt83, que compartilharam 96% de semelhança genética, colônias cotonosas e alta virulência. Essa técnica pode discriminar apenas isolados com morfologia glabra da cotonosa e com alta e baixa virulência. Isolados com virulência intermediária como Pb18, Pb18B6, Bt32 e Bt54 mostraram variabilidade no coeficiente de similaridade, sugerindo que a técnica de RAPD permite mostrar variabilidade genética nessa espécie fúngica. O estudo do perfil de virulência das amostras de P. brasiliensis demonstrou que os dois fenótipos extremos de morfologia miceliana podem ser associados com a virulência do fungo e com o tempo de subcultivo in vitro. Assim, a análise de RAPD, utilizada em conjunto com aspectos de virulência, morfológicos e imunológicos pode ser considerada adequada para detectar diferenças entre isolados de P. brasiliensis. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30935Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 No. 5 (2005); 257-262 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 Núm. 5 (2005); 257-262 Revista do Instituto de Medicina Tropical de São Paulo; v. 47 n. 5 (2005); 257-262 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30935/32819Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessKurokawa, Cilmery S.Lopes, Catalina R.Sugizaki, Maria F.Kuramae, Eiko E.Franco, Marcello F.Peraçoli, Maria T. S.2012-07-07T18:38:08Zoai:revistas.usp.br:article/30935Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:39.564868Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
Perfil de virulência de dez isolados de Paracoccidioides brasiliensis: associação com morfologia e padrão genético
title Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
spellingShingle Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
Kurokawa, Cilmery S.
Antibodies
Colony morphology
Paracoccidioides brasiliensis
RAPD
Virulence
title_short Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
title_full Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
title_fullStr Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
title_full_unstemmed Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
title_sort Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns
author Kurokawa, Cilmery S.
author_facet Kurokawa, Cilmery S.
Lopes, Catalina R.
Sugizaki, Maria F.
Kuramae, Eiko E.
Franco, Marcello F.
Peraçoli, Maria T. S.
author_role author
author2 Lopes, Catalina R.
Sugizaki, Maria F.
Kuramae, Eiko E.
Franco, Marcello F.
Peraçoli, Maria T. S.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kurokawa, Cilmery S.
Lopes, Catalina R.
Sugizaki, Maria F.
Kuramae, Eiko E.
Franco, Marcello F.
Peraçoli, Maria T. S.
dc.subject.por.fl_str_mv Antibodies
Colony morphology
Paracoccidioides brasiliensis
RAPD
Virulence
topic Antibodies
Colony morphology
Paracoccidioides brasiliensis
RAPD
Virulence
description Ten isolates of Paracoccidioides brasiliensis were examined for differences in virulence in outbred mice intravenously inoculated with the fungus, associated with mycelial morphology, and genetic patterns measured by random amplified polymorphic DNA (RAPD). Virulence was evaluated by viable yeast cell recovery from lungs and demonstration of histopathologic lesions in different organs. The results showed that the isolates presented four virulence degrees: high virulence, intermediate, low and non-virulence. RAPD clustered the isolates studied in two main groups with 56% of genetic similarity. Strains with low virulence, Pb265 or the non-virulent, Pb192, showed glabrous/cerebriform morphology and high genetic similarity (98.7%) when compared to the other isolates studied. The same was observed with Bt79 and Bt83 that shared 96% genetic similarity, cottony colonies and high virulence. The RAPD technique could only discriminate P. brasiliensis isolates according to glabrous/cerebriform or cottony colonies, and also high from low virulence strains. Isolates with intermediate virulence such as Pb18, Pb18B6, Bt32 and Bt56 showed variability in their similarity coefficient suggesting that RAPD was able to detect genetic variability in this fungal specie. Virulence profile of P. brasiliensis demonstrated that both mycelial morphologic extreme phenotypes may be associated with fungal virulence and their in vitro subculture time. Thus, RAPD technique analysis employed in association with virulence, morphologic and immunologic aspects might prove adequate to detect differences between P. brasiliensis isolates.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30935
url https://www.revistas.usp.br/rimtsp/article/view/30935
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30935/32819
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 No. 5 (2005); 257-262
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 47 Núm. 5 (2005); 257-262
Revista do Instituto de Medicina Tropical de São Paulo; v. 47 n. 5 (2005); 257-262
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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