Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations

Detalhes bibliográficos
Autor(a) principal: Ortiz-Princz,Diana
Data de Publicação: 2010
Outros Autores: Guariglia-Oropeza,Verónica, Ávila,Maira, Correnti,María, Perrone,Marianella, Gutierrez,Beatriz, Torres,Javier, Megraud,Francis, Cavazza,María Eugenia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300016
Resumo: The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segments was performed using two regions of cagA: 349 bp were amplified with the F1/B1 primers and the remaining 335 bp were amplified with the B7629/B7628 primers. The VA1-F/VA1-R set of primers was used to amplify the 259-bp (s1) or 286-bp (s2) product and the VAG-R/VAG-F set of primers was used to amplify the 567-bp (m1) or 642-bp (m2) regions of vacA. cagA was detected in 87% of the antral samples from Cuban patients and 80.3% of those from Venezuelan patients. All possible combinations of vacA regions were found, with the exception of s2/m1. The predominant combination found in both countries was s1/m1. The percentage of cagA+ strains was increased by the use of a second set of primers and a greater number of strains was amplified with the B7629/B7628 primers in the Cuban patients (p = 0.0001). There was no significant difference between the presence of the allelic variants of vacA and cagA in both populations. The predominant genotype was cagA+/s1m1 in both countries. The results support the necessary investigation of isolates circulating among the human population in each region.
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spelling Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populationsHelicobacter pyloricagAvacAchronic gastritisThe aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segments was performed using two regions of cagA: 349 bp were amplified with the F1/B1 primers and the remaining 335 bp were amplified with the B7629/B7628 primers. The VA1-F/VA1-R set of primers was used to amplify the 259-bp (s1) or 286-bp (s2) product and the VAG-R/VAG-F set of primers was used to amplify the 567-bp (m1) or 642-bp (m2) regions of vacA. cagA was detected in 87% of the antral samples from Cuban patients and 80.3% of those from Venezuelan patients. All possible combinations of vacA regions were found, with the exception of s2/m1. The predominant combination found in both countries was s1/m1. The percentage of cagA+ strains was increased by the use of a second set of primers and a greater number of strains was amplified with the B7629/B7628 primers in the Cuban patients (p = 0.0001). There was no significant difference between the presence of the allelic variants of vacA and cagA in both populations. The predominant genotype was cagA+/s1m1 in both countries. The results support the necessary investigation of isolates circulating among the human population in each region.Instituto Oswaldo Cruz, Ministério da Saúde2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300016Memórias do Instituto Oswaldo Cruz v.105 n.3 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000300016info:eu-repo/semantics/openAccessOrtiz-Princz,DianaGuariglia-Oropeza,VerónicaÁvila,MairaCorrenti,MaríaPerrone,MarianellaGutierrez,BeatrizTorres,JavierMegraud,FrancisCavazza,María Eugeniaeng2020-04-25T17:50:47Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:55.996Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
title Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
spellingShingle Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
Ortiz-Princz,Diana
Helicobacter pylori
cagA
vacA
chronic gastritis
title_short Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
title_full Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
title_fullStr Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
title_full_unstemmed Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
title_sort Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations
author Ortiz-Princz,Diana
author_facet Ortiz-Princz,Diana
Guariglia-Oropeza,Verónica
Ávila,Maira
Correnti,María
Perrone,Marianella
Gutierrez,Beatriz
Torres,Javier
Megraud,Francis
Cavazza,María Eugenia
author_role author
author2 Guariglia-Oropeza,Verónica
Ávila,Maira
Correnti,María
Perrone,Marianella
Gutierrez,Beatriz
Torres,Javier
Megraud,Francis
Cavazza,María Eugenia
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ortiz-Princz,Diana
Guariglia-Oropeza,Verónica
Ávila,Maira
Correnti,María
Perrone,Marianella
Gutierrez,Beatriz
Torres,Javier
Megraud,Francis
Cavazza,María Eugenia
dc.subject.por.fl_str_mv Helicobacter pylori
cagA
vacA
chronic gastritis
topic Helicobacter pylori
cagA
vacA
chronic gastritis
dc.description.none.fl_txt_mv The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segments was performed using two regions of cagA: 349 bp were amplified with the F1/B1 primers and the remaining 335 bp were amplified with the B7629/B7628 primers. The VA1-F/VA1-R set of primers was used to amplify the 259-bp (s1) or 286-bp (s2) product and the VAG-R/VAG-F set of primers was used to amplify the 567-bp (m1) or 642-bp (m2) regions of vacA. cagA was detected in 87% of the antral samples from Cuban patients and 80.3% of those from Venezuelan patients. All possible combinations of vacA regions were found, with the exception of s2/m1. The predominant combination found in both countries was s1/m1. The percentage of cagA+ strains was increased by the use of a second set of primers and a greater number of strains was amplified with the B7629/B7628 primers in the Cuban patients (p = 0.0001). There was no significant difference between the presence of the allelic variants of vacA and cagA in both populations. The predominant genotype was cagA+/s1m1 in both countries. The results support the necessary investigation of isolates circulating among the human population in each region.
description The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segments was performed using two regions of cagA: 349 bp were amplified with the F1/B1 primers and the remaining 335 bp were amplified with the B7629/B7628 primers. The VA1-F/VA1-R set of primers was used to amplify the 259-bp (s1) or 286-bp (s2) product and the VAG-R/VAG-F set of primers was used to amplify the 567-bp (m1) or 642-bp (m2) regions of vacA. cagA was detected in 87% of the antral samples from Cuban patients and 80.3% of those from Venezuelan patients. All possible combinations of vacA regions were found, with the exception of s2/m1. The predominant combination found in both countries was s1/m1. The percentage of cagA+ strains was increased by the use of a second set of primers and a greater number of strains was amplified with the B7629/B7628 primers in the Cuban patients (p = 0.0001). There was no significant difference between the presence of the allelic variants of vacA and cagA in both populations. The predominant genotype was cagA+/s1m1 in both countries. The results support the necessary investigation of isolates circulating among the human population in each region.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000300016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.3 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
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collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
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repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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