Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Jobe,Momodou
Data de Publicação: 2012
Outros Autores: Anwuzia-Iwegbu,Charles, Banful,Ama, Bosier,Emma, Iqbal,Mubeen, Jones,Kelly, Lecutier,Suzanne J, Lepper,Kasimir, Redmond,Matt, Ross-Parker,Andrew, Ward,Emily, Wernham,Paul, Whidden,Eleanor M, Tyler,Kevin M, Steverding,Dietmar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700018
Resumo: In this study the effect of eight DNA topoisomerase inhibitors on the growth Trypanosoma rangeli epimastigotes in cell culture was investigated. Among the eight compounds tested, idarubicin was the only compound that displayed promising trypanocidal activity with a half-maximal growth inhibition (GI50) value in the sub-micromolar range. Fluorescence-activated cell sorting analysis showed a reduction in DNA content in T. rangeli epimastigotes when treated with idarubicin. In contrast to T. rangeli, against Trypanosoma cruzi epimastigotes idarubicin was much less effective exhibiting a GI50 value in the mid-micromolar range. This result indicates that idarubicin displays differential toxic effects in T. rangeli and T. cruzi. Compared with African trypanosomes, it seems that American trypanosomes are generally less susceptible to DNA topoisomerase inhibitors.
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spelling Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruziTrypanosoma rangeliTrypanosoma cruziChagas diseaseDNA topoisomerase inhibitorsdrug screeningchemotherapyIn this study the effect of eight DNA topoisomerase inhibitors on the growth Trypanosoma rangeli epimastigotes in cell culture was investigated. Among the eight compounds tested, idarubicin was the only compound that displayed promising trypanocidal activity with a half-maximal growth inhibition (GI50) value in the sub-micromolar range. Fluorescence-activated cell sorting analysis showed a reduction in DNA content in T. rangeli epimastigotes when treated with idarubicin. In contrast to T. rangeli, against Trypanosoma cruzi epimastigotes idarubicin was much less effective exhibiting a GI50 value in the mid-micromolar range. This result indicates that idarubicin displays differential toxic effects in T. rangeli and T. cruzi. Compared with African trypanosomes, it seems that American trypanosomes are generally less susceptible to DNA topoisomerase inhibitors.Instituto Oswaldo Cruz, Ministério da Saúde2012-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700018Memórias do Instituto Oswaldo Cruz v.107 n.7 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000700018info:eu-repo/semantics/openAccessJobe,MomodouAnwuzia-Iwegbu,CharlesBanful,AmaBosier,EmmaIqbal,MubeenJones,KellyLecutier,Suzanne JLepper,KasimirRedmond,MattRoss-Parker,AndrewWard,EmilyWernham,PaulWhidden,Eleanor MTyler,Kevin MSteverding,Dietmareng2020-04-25T17:51:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:35.774Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
title Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
spellingShingle Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
Jobe,Momodou
Trypanosoma rangeli
Trypanosoma cruzi
Chagas disease
DNA topoisomerase inhibitors
drug screening
chemotherapy
title_short Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
title_full Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
title_fullStr Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
title_full_unstemmed Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
title_sort Differential in vitro activity of the DNA topoisomerase inhibitor idarubicin against Trypanosoma rangeli and Trypanosoma cruzi
author Jobe,Momodou
author_facet Jobe,Momodou
Anwuzia-Iwegbu,Charles
Banful,Ama
Bosier,Emma
Iqbal,Mubeen
Jones,Kelly
Lecutier,Suzanne J
Lepper,Kasimir
Redmond,Matt
Ross-Parker,Andrew
Ward,Emily
Wernham,Paul
Whidden,Eleanor M
Tyler,Kevin M
Steverding,Dietmar
author_role author
author2 Anwuzia-Iwegbu,Charles
Banful,Ama
Bosier,Emma
Iqbal,Mubeen
Jones,Kelly
Lecutier,Suzanne J
Lepper,Kasimir
Redmond,Matt
Ross-Parker,Andrew
Ward,Emily
Wernham,Paul
Whidden,Eleanor M
Tyler,Kevin M
Steverding,Dietmar
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jobe,Momodou
Anwuzia-Iwegbu,Charles
Banful,Ama
Bosier,Emma
Iqbal,Mubeen
Jones,Kelly
Lecutier,Suzanne J
Lepper,Kasimir
Redmond,Matt
Ross-Parker,Andrew
Ward,Emily
Wernham,Paul
Whidden,Eleanor M
Tyler,Kevin M
Steverding,Dietmar
dc.subject.por.fl_str_mv Trypanosoma rangeli
Trypanosoma cruzi
Chagas disease
DNA topoisomerase inhibitors
drug screening
chemotherapy
topic Trypanosoma rangeli
Trypanosoma cruzi
Chagas disease
DNA topoisomerase inhibitors
drug screening
chemotherapy
dc.description.none.fl_txt_mv In this study the effect of eight DNA topoisomerase inhibitors on the growth Trypanosoma rangeli epimastigotes in cell culture was investigated. Among the eight compounds tested, idarubicin was the only compound that displayed promising trypanocidal activity with a half-maximal growth inhibition (GI50) value in the sub-micromolar range. Fluorescence-activated cell sorting analysis showed a reduction in DNA content in T. rangeli epimastigotes when treated with idarubicin. In contrast to T. rangeli, against Trypanosoma cruzi epimastigotes idarubicin was much less effective exhibiting a GI50 value in the mid-micromolar range. This result indicates that idarubicin displays differential toxic effects in T. rangeli and T. cruzi. Compared with African trypanosomes, it seems that American trypanosomes are generally less susceptible to DNA topoisomerase inhibitors.
description In this study the effect of eight DNA topoisomerase inhibitors on the growth Trypanosoma rangeli epimastigotes in cell culture was investigated. Among the eight compounds tested, idarubicin was the only compound that displayed promising trypanocidal activity with a half-maximal growth inhibition (GI50) value in the sub-micromolar range. Fluorescence-activated cell sorting analysis showed a reduction in DNA content in T. rangeli epimastigotes when treated with idarubicin. In contrast to T. rangeli, against Trypanosoma cruzi epimastigotes idarubicin was much less effective exhibiting a GI50 value in the mid-micromolar range. This result indicates that idarubicin displays differential toxic effects in T. rangeli and T. cruzi. Compared with African trypanosomes, it seems that American trypanosomes are generally less susceptible to DNA topoisomerase inhibitors.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000700018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762012000700018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.107 n.7 2012
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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