Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

Detalhes bibliográficos
Autor(a) principal: Mendonça,Marcos César Lima de
Data de Publicação: 2011
Outros Autores: Ferreira,Ana Maria de Amorim, Santos,Marta Gonçalves Matos dos, Oviedo,Elva Cristina, Bello,Maria Sônia Dal, Siqueira,Marilda Mendonça, Maceira,Juan Manuel Piñeiro, von Hubinger,Maria Genoveva, Couceiro,José Nelson dos Santos Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019
Resumo: Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.
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spelling Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencingparvovirus B19nucleotide sequencinggenotypingHMASSCPHeteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.Instituto Oswaldo Cruz, Ministério da Saúde2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019Memórias do Instituto Oswaldo Cruz v.106 n.4 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000400019info:eu-repo/semantics/openAccessMendonça,Marcos César Lima deFerreira,Ana Maria de AmorimSantos,Marta Gonçalves Matos dosOviedo,Elva CristinaBello,Maria Sônia DalSiqueira,Marilda MendonçaMaceira,Juan Manuel Piñeirovon Hubinger,Maria GenovevaCouceiro,José Nelson dos Santos Silvaeng2020-04-25T17:51:01Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:42.839Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
spellingShingle Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
Mendonça,Marcos César Lima de
parvovirus B19
nucleotide sequencing
genotyping
HMA
SSCP
title_short Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_full Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_fullStr Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_full_unstemmed Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
title_sort Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing
author Mendonça,Marcos César Lima de
author_facet Mendonça,Marcos César Lima de
Ferreira,Ana Maria de Amorim
Santos,Marta Gonçalves Matos dos
Oviedo,Elva Cristina
Bello,Maria Sônia Dal
Siqueira,Marilda Mendonça
Maceira,Juan Manuel Piñeiro
von Hubinger,Maria Genoveva
Couceiro,José Nelson dos Santos Silva
author_role author
author2 Ferreira,Ana Maria de Amorim
Santos,Marta Gonçalves Matos dos
Oviedo,Elva Cristina
Bello,Maria Sônia Dal
Siqueira,Marilda Mendonça
Maceira,Juan Manuel Piñeiro
von Hubinger,Maria Genoveva
Couceiro,José Nelson dos Santos Silva
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mendonça,Marcos César Lima de
Ferreira,Ana Maria de Amorim
Santos,Marta Gonçalves Matos dos
Oviedo,Elva Cristina
Bello,Maria Sônia Dal
Siqueira,Marilda Mendonça
Maceira,Juan Manuel Piñeiro
von Hubinger,Maria Genoveva
Couceiro,José Nelson dos Santos Silva
dc.subject.por.fl_str_mv parvovirus B19
nucleotide sequencing
genotyping
HMA
SSCP
topic parvovirus B19
nucleotide sequencing
genotyping
HMA
SSCP
dc.description.none.fl_txt_mv Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.
description Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000400019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.4 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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