Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA

Detalhes bibliográficos
Autor(a) principal: Cuervo,Patricia
Data de Publicação: 2002
Outros Autores: Cupolillo,Elisa, Segura,Iris, Saravia,Nancy, Fernandes,Octavio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600023
Resumo: American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.
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spelling Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNATrypanosoma cruziinternal transcribed spacersmini-exon generibosomal DNAColombiaAmerican trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.Instituto Oswaldo Cruz, Ministério da Saúde2002-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600023Memórias do Instituto Oswaldo Cruz v.97 n.6 2002reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762002000600023info:eu-repo/semantics/openAccessCuervo,PatriciaCupolillo,ElisaSegura,IrisSaravia,NancyFernandes,Octavioeng2020-04-25T17:48:51Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:21.736Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
title Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
spellingShingle Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
Cuervo,Patricia
Trypanosoma cruzi
internal transcribed spacers
mini-exon gene
ribosomal DNA
Colombia
title_short Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
title_full Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
title_fullStr Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
title_full_unstemmed Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
title_sort Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA
author Cuervo,Patricia
author_facet Cuervo,Patricia
Cupolillo,Elisa
Segura,Iris
Saravia,Nancy
Fernandes,Octavio
author_role author
author2 Cupolillo,Elisa
Segura,Iris
Saravia,Nancy
Fernandes,Octavio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cuervo,Patricia
Cupolillo,Elisa
Segura,Iris
Saravia,Nancy
Fernandes,Octavio
dc.subject.por.fl_str_mv Trypanosoma cruzi
internal transcribed spacers
mini-exon gene
ribosomal DNA
Colombia
topic Trypanosoma cruzi
internal transcribed spacers
mini-exon gene
ribosomal DNA
Colombia
dc.description.none.fl_txt_mv American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.
description American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.
publishDate 2002
dc.date.none.fl_str_mv 2002-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600023
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762002000600023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.97 n.6 2002
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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