Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA

Detalhes bibliográficos
Autor(a) principal: Cuervo, Patrícia
Data de Publicação: 2002
Outros Autores: Cupolillo, Elisa, Segura, Iris, Saravia, Nancy, Fernandes, Octavio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44209
Resumo: Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia.
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spelling Cuervo, PatríciaCupolillo, ElisaSegura, IrisSaravia, NancyFernandes, Octavio2020-10-26T18:56:13Z2020-10-26T18:56:13Z2002CUERVO, Patricia et al. Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 877-880, Sept. 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4420910.1590/S0074-027620020006000231678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziEspaçadores transcritos internosMini-exon geneDNA ribossômicoColômbiaTrypanosoma cruziInternal transcribed spacersMini-exon geneRibosomal DNAColombiaGenetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia.Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia.Universidade do Estado de Rio de Janeiro. Rio de Janeiro, RJ, Brasil.American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44209/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALElisaCupolillo_PatriciaCuervo_etal_IOC_2002.pdfElisaCupolillo_PatriciaCuervo_etal_IOC_2002.pdfapplication/pdf36934https://www.arca.fiocruz.br/bitstream/icict/44209/2/ElisaCupolillo_PatriciaCuervo_etal_IOC_2002.pdfcb8c1f342d7585c96c84a2c703afba98MD52TEXTElisaCupolillo_PatriciaCuervo_etal_IOC_2002.pdf.txtElisaCupolillo_PatriciaCuervo_etal_IOC_2002.pdf.txtExtracted texttext/plain18048https://www.arca.fiocruz.br/bitstream/icict/44209/3/ElisaCupolillo_PatriciaCuervo_etal_IOC_2002.pdf.txt60f7a9e4093a5fffd962ea8c7bbd70d1MD53icict/442092020-10-27 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dc.title.pt_BR.fl_str_mv Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
title Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
spellingShingle Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
Cuervo, Patrícia
Trypanosoma cruzi
Espaçadores transcritos internos
Mini-exon gene
DNA ribossômico
Colômbia
Trypanosoma cruzi
Internal transcribed spacers
Mini-exon gene
Ribosomal DNA
Colombia
title_short Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
title_full Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
title_fullStr Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
title_full_unstemmed Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
title_sort Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA
author Cuervo, Patrícia
author_facet Cuervo, Patrícia
Cupolillo, Elisa
Segura, Iris
Saravia, Nancy
Fernandes, Octavio
author_role author
author2 Cupolillo, Elisa
Segura, Iris
Saravia, Nancy
Fernandes, Octavio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cuervo, Patrícia
Cupolillo, Elisa
Segura, Iris
Saravia, Nancy
Fernandes, Octavio
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Espaçadores transcritos internos
Mini-exon gene
DNA ribossômico
Colômbia
topic Trypanosoma cruzi
Espaçadores transcritos internos
Mini-exon gene
DNA ribossômico
Colômbia
Trypanosoma cruzi
Internal transcribed spacers
Mini-exon gene
Ribosomal DNA
Colombia
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Internal transcribed spacers
Mini-exon gene
Ribosomal DNA
Colombia
description Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-10-26T18:56:13Z
dc.date.available.fl_str_mv 2020-10-26T18:56:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUERVO, Patricia et al. Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 877-880, Sept. 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44209
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762002000600023
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CUERVO, Patricia et al. Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 877-880, Sept. 2002.
0074-0276
10.1590/S0074-02762002000600023
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44209
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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