Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice

Detalhes bibliográficos
Autor(a) principal: Serafim,Tiago Donatelli
Data de Publicação: 2010
Outros Autores: Malafaia,Guilherme, Silva,Marcelo Eustáquio, Pedrosa,Maria Lúcia, Rezende,Simone Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014
Resumo: Protein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals.
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spelling Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c micevisceral leishmaniasismalnutritionLeishmania chagasiBALB/cinterleukin 4interferon-gammaProtein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals.Instituto Oswaldo Cruz, Ministério da Saúde2010-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014Memórias do Instituto Oswaldo Cruz v.105 n.6 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000600014info:eu-repo/semantics/openAccessSerafim,Tiago DonatelliMalafaia,GuilhermeSilva,Marcelo EustáquioPedrosa,Maria LúciaRezende,Simone Aparecidaeng2020-04-25T17:50:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:15.667Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
title Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
spellingShingle Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
Serafim,Tiago Donatelli
visceral leishmaniasis
malnutrition
Leishmania chagasi
BALB/c
interleukin 4
interferon-gamma
title_short Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
title_full Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
title_fullStr Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
title_full_unstemmed Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
title_sort Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
author Serafim,Tiago Donatelli
author_facet Serafim,Tiago Donatelli
Malafaia,Guilherme
Silva,Marcelo Eustáquio
Pedrosa,Maria Lúcia
Rezende,Simone Aparecida
author_role author
author2 Malafaia,Guilherme
Silva,Marcelo Eustáquio
Pedrosa,Maria Lúcia
Rezende,Simone Aparecida
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Serafim,Tiago Donatelli
Malafaia,Guilherme
Silva,Marcelo Eustáquio
Pedrosa,Maria Lúcia
Rezende,Simone Aparecida
dc.subject.por.fl_str_mv visceral leishmaniasis
malnutrition
Leishmania chagasi
BALB/c
interleukin 4
interferon-gamma
topic visceral leishmaniasis
malnutrition
Leishmania chagasi
BALB/c
interleukin 4
interferon-gamma
dc.description.none.fl_txt_mv Protein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals.
description Protein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000600014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.6 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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