Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014 |
Resumo: | Protein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals. |
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Memórias do Instituto Oswaldo Cruz |
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Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c micevisceral leishmaniasismalnutritionLeishmania chagasiBALB/cinterleukin 4interferon-gammaProtein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals.Instituto Oswaldo Cruz, Ministério da Saúde2010-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014Memórias do Instituto Oswaldo Cruz v.105 n.6 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000600014info:eu-repo/semantics/openAccessSerafim,Tiago DonatelliMalafaia,GuilhermeSilva,Marcelo EustáquioPedrosa,Maria LúciaRezende,Simone Aparecidaeng2020-04-25T17:50:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:15.667Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
title |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
spellingShingle |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice Serafim,Tiago Donatelli visceral leishmaniasis malnutrition Leishmania chagasi BALB/c interleukin 4 interferon-gamma |
title_short |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
title_full |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
title_fullStr |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
title_full_unstemmed |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
title_sort |
Immune response to Leishmania (Leishmania) chagasi infection is reduced in malnourished BALB/c mice |
author |
Serafim,Tiago Donatelli |
author_facet |
Serafim,Tiago Donatelli Malafaia,Guilherme Silva,Marcelo Eustáquio Pedrosa,Maria Lúcia Rezende,Simone Aparecida |
author_role |
author |
author2 |
Malafaia,Guilherme Silva,Marcelo Eustáquio Pedrosa,Maria Lúcia Rezende,Simone Aparecida |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Serafim,Tiago Donatelli Malafaia,Guilherme Silva,Marcelo Eustáquio Pedrosa,Maria Lúcia Rezende,Simone Aparecida |
dc.subject.por.fl_str_mv |
visceral leishmaniasis malnutrition Leishmania chagasi BALB/c interleukin 4 interferon-gamma |
topic |
visceral leishmaniasis malnutrition Leishmania chagasi BALB/c interleukin 4 interferon-gamma |
dc.description.none.fl_txt_mv |
Protein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals. |
description |
Protein-energy malnutrition and micronutrient deficiencies may down-regulate immune response and increase morbidity and mortality due to infection. In this study, a murine model was used to study the effects of protein, iron and zinc deficiencies on the immune response to Leishmania (Leishmania) chagasi infection. Mice were initially fed a standard diet or with a diet containing 3% casein but deficient in zinc and iron. After malnutrition was established, mice were inoculated with L. chagasiand sacrificed four weeks later in order to evaluate liver and spleen parasite loads and serum biochemical parameters. Significant decreases in liver and spleen weight, an increase in the parasite loads in these organs and decreases in serum protein and glucose concentrations in malnourished animals were observed. Furthermore, the production of interferon-gamma by spleen cells from infected malnourished mice stimulated by Leishmaniaantigen was significantly lower compared with that in control diet mice. These data suggest that malnutrition alters the immune response to L. chagasiinfection in the BALB/c model and, in association with the effects on biochemical and anatomical parameters of the host, favored increases in the parasite loads in the spleens and livers of these animals. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762010000600014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.105 n.6 2010 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937708760301568 |