Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/7152 |
Resumo: | p. 977-984 |
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Oliveira, Camila Indiani deTeixeira, Maria JaniaTeixeira, Clarissa RomeroJesus, Joílson Ramos deRosato, Andréa BomuraSilva, João Santa daBrodskyn, Claudia IdaBarral-Netto, ManoelBarral, Aldina Maria PradoOliveira, Camila Indiani deTeixeira, Maria JaniaTeixeira, Clarissa RomeroJesus, Joílson Ramos deRosato, Andréa BomuraSilva, João Santa daBrodskyn, Claudia IdaBarral-Netto, ManoelBarral, Aldina Maria Prado2012-11-08T18:46:05Z20041286-4579http://www.repositorio.ufba.br/ri/handle/ri/7152v. 6, n. 1p. 977-984Leishmania braziliensis is the species responsible for the majority of cases of human cutaneous leishmaniasis in Brazil. In the present study, L. braziliensis isolates from two different geographic areas in Brazil were studied by RAPD, using arbitrary primers.We also evaluated other biological features of these two isolates. We compared (a) the clinical features they initiate or not once delivered subcutaneously as stationary-phase promastigotes in the footpad of BALB/c mice; (b) the parasite load in both the footpad and the draining lymph node; (c) the cytokines present in the supernatant of cultures of the cell suspensions from the draining lymph nodes; and (d) the cell types present at the site of parasite delivery. The results show that the L. braziliensis strain from Ceará (H3227) is genotypically different from the L. braziliensis strain from Bahia (BA788). H3227-parasitized mice developed detectable lesions, whereas BA788-parasitized mice did not. Fifteen days post parasite inoculation there was an increase in the numbers of macrophages and lymphocytes in the footpads, whatever the parasite inoculum. Parasite load at the inoculation site—namely the footpad—did not differ significantly; in draining lymph nodes, however, it increased over the period under study. Early after parasite inoculation, the cells recovered from the draining lymph nodes of BA788-parasitized mice produced higher levels of IFN-c, a feature coupled to a higher number of NK cells. Later, after the parasite inoculation, there was an increased content of IL-12p70 and IL-10 in the supernatant of cells recovered from the lymph nodes of H3227-parasitized mice. This comparative analysis points out that L. braziliensis isolates differing in their genomic profiles do establish different parasitic processes in BALB/c mice.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-11-08T18:46:05Z No. of bitstreams: 1 1-s2.0-S1286457904001856-main.pdf: 320965 bytes, checksum: aef854a2cfd36c43568ef089a238616a (MD5)Made available in DSpace on 2012-11-08T18:46:05Z (GMT). No. of bitstreams: 1 1-s2.0-S1286457904001856-main.pdf: 320965 bytes, checksum: aef854a2cfd36c43568ef089a238616a (MD5) Previous issue date: 2004http://dx.doi.org/10.1016/j.micinf.2004.05.009reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania braziliensisRandom amplification of polymorphic DNAInterferon gammaInterleukin-12p70Interleukin-10Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c miceMicrobes and Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S1286457904001856-main.pdf1-s2.0-S1286457904001856-main.pdfapplication/pdf320965https://repositorio.ufba.br/bitstream/ri/7152/1/1-s2.0-S1286457904001856-main.pdfaef854a2cfd36c43568ef089a238616aMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/7152/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT1-s2.0-S1286457904001856-main.pdf.txt1-s2.0-S1286457904001856-main.pdf.txtExtracted texttext/plain35877https://repositorio.ufba.br/bitstream/ri/7152/3/1-s2.0-S1286457904001856-main.pdf.txtcdd0fd66149726a0ee890759161191fcMD53ri/71522022-07-05 14:03:44.263oai:repositorio.ufba.br:ri/7152VGVybW8gZGUgTGljZW7Dp2EsIG7Do28gZXhjbHVzaXZvLCBwYXJhIG8gZGVww7NzaXRvIG5vIHJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGQkEKCiAgICBQZWxvIHByb2Nlc3NvIGRlIHN1Ym1pc3PDo28gZGUgZG9jdW1lbnRvcywgbyBhdXRvciBvdSBzZXUKcmVwcmVzZW50YW50ZSBsZWdhbCwgYW8gYWNlaXRhciBlc3NlIHRlcm1vIGRlIGxpY2Vuw6dhLCBjb25jZWRlIGFvClJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRhIEJhaGlhIG8gZGlyZWl0bwpkZSBtYW50ZXIgdW1hIGPDs3BpYSBlbSBzZXUgcmVwb3NpdMOzcmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHDp8Ojby4gRXNzZXMgdGVybW9zLCBuw6NvIGV4Y2x1c2l2b3MsIG1hbnTDqm0gb3MgZGlyZWl0b3MgZGUgCmF1dG9yL2NvcHlyaWdodCwgbWFzIGVudGVuZGUgbyBkb2N1bWVudG8gY29tbyBwYXJ0ZSBkbyBhY2Vydm8gaW50ZWxlY3R1YWwgZGVzc2EgVW5pdmVyc2lkYWRlLiAKCiAgICBQYXJhIG9zIGRvY3VtZW50b3MgcHVibGljYWRvcyBjb20gcmVwYXNzZSBkZSBkaXJlaXRvcyBkZSBkaXN0cmlidWnDp8OjbywgZXNzZSB0ZXJtbyBkZSBsaWNlbsOnYSBlbnRlbmRlIHF1ZTogCgogICAgTWFudGVuZG8gb3MgIGRpcmVpdG9zIGF1dG9yYWlzLCByZXBhc3NhZG9zIGEgdGVyY2Vpcm9zLCBlbSBjYXNvIApkZSBwdWJsaWNhw6fDtWVzLCBvIHJlcG9zaXTDs3JpbyBwb2RlIHJlc3RyaW5naXIgbyBhY2Vzc28gYW8gdGV4dG8gCmludGVncmFsLCBtYXMgbGliZXJhIGFzIGluZm9ybWHDp8O1ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXDp8OjbyBjaWVudMOtZmljYSBjb20gYXMgcmVzdHJpw6fDtWVzIGltcG9zdGFzIHBlbG9zIAplZGl0b3JlcyBkZSBwZXJpw7NkaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNhw6fDtWVzIGVtIGluaWNpYXRpdmFzIHF1ZSBzZWd1ZW0gYSBwb2zDrcKsdGljYSBkZSAKQWNlc3NvIEFiZXJ0bywgb3MgZGVww7NzaXRvcyBjb21wdWxzw7NyaW9zIG5lc3NlIHJlcG9zaXTDs3JpbyBtYW50w6ptIApvcyBkaXJlaXRvcyBhdXRvcmFpcywgbWFzIG1hbnTDqW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0YcOnw6NvIGRlc3NlIHRlcm1vIG7Do28gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7Dp2EsIHBvZGUgc2VyIGFjZWl0byBwZWxvIAphdXRvciwgZGV0ZW50b3JlcyBkZSBkaXJlaXRvcyBlL291IHRlcmNlaXJvcyBhbXBhcmFkb3MgcGVsYSAKdW5pdmVyc2lkYWRlLiBEZXZpZG8gYW9zIGRpZmVyZW50ZXMgcHJvY2Vzc29zIHBlbG8gcXVhbCBhIHN1Ym1pc3PDo28gCnBvZGUgb2NvcnJlciwgbyByZXBvc2l0w7NyaW8gcGVybWl0ZSBhIGFjZWl0YcOnw6NvIGRhIGxpY2Vuw6dhIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnDp8Ojby4KRepositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:44Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| dc.title.alternative.pt_BR.fl_str_mv |
Microbes and Infection |
| title |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| spellingShingle |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice Oliveira, Camila Indiani de Leishmania braziliensis Random amplification of polymorphic DNA Interferon gamma Interleukin-12p70 Interleukin-10 |
| title_short |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| title_full |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| title_fullStr |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| title_full_unstemmed |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| title_sort |
Leishmania braziliensis isolates differing at the genome level display distinctive features in BALB/c mice |
| author |
Oliveira, Camila Indiani de |
| author_facet |
Oliveira, Camila Indiani de Teixeira, Maria Jania Teixeira, Clarissa Romero Jesus, Joílson Ramos de Rosato, Andréa Bomura Silva, João Santa da Brodskyn, Claudia Ida Barral-Netto, Manoel Barral, Aldina Maria Prado |
| author_role |
author |
| author2 |
Teixeira, Maria Jania Teixeira, Clarissa Romero Jesus, Joílson Ramos de Rosato, Andréa Bomura Silva, João Santa da Brodskyn, Claudia Ida Barral-Netto, Manoel Barral, Aldina Maria Prado |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Camila Indiani de Teixeira, Maria Jania Teixeira, Clarissa Romero Jesus, Joílson Ramos de Rosato, Andréa Bomura Silva, João Santa da Brodskyn, Claudia Ida Barral-Netto, Manoel Barral, Aldina Maria Prado Oliveira, Camila Indiani de Teixeira, Maria Jania Teixeira, Clarissa Romero Jesus, Joílson Ramos de Rosato, Andréa Bomura Silva, João Santa da Brodskyn, Claudia Ida Barral-Netto, Manoel Barral, Aldina Maria Prado |
| dc.subject.por.fl_str_mv |
Leishmania braziliensis Random amplification of polymorphic DNA Interferon gamma Interleukin-12p70 Interleukin-10 |
| topic |
Leishmania braziliensis Random amplification of polymorphic DNA Interferon gamma Interleukin-12p70 Interleukin-10 |
| description |
p. 977-984 |
| publishDate |
2004 |
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2004 |
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2012-11-08T18:46:05Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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1286-4579 |
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v. 6, n. 1 |
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1286-4579 v. 6, n. 1 |
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http://www.repositorio.ufba.br/ri/handle/ri/7152 |
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http://dx.doi.org/10.1016/j.micinf.2004.05.009 |
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