Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

Detalhes bibliográficos
Autor(a) principal: Marinheiro,Juliana Cristina
Data de Publicação: 2009
Outros Autores: Sanalios,Roberta Braga, Santos,Daniela Carvalho dos, Costa,Cristovão Alves da, Hársi,Charlotte Marianna
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000100018
Resumo: Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
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spelling Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal sampleshuman adenovirushuman respiratory syncytial virusacute respiratory infectionpolymerase chain reactionHuman adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.Instituto Oswaldo Cruz, Ministério da Saúde2009-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000100018Memórias do Instituto Oswaldo Cruz v.104 n.1 2009reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762009000100018info:eu-repo/semantics/openAccessMarinheiro,Juliana CristinaSanalios,Roberta BragaSantos,Daniela Carvalho dosCosta,Cristovão Alves daHársi,Charlotte Mariannaeng2020-04-25T17:50:26Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:01.407Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
spellingShingle Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Marinheiro,Juliana Cristina
human adenovirus
human respiratory syncytial virus
acute respiratory infection
polymerase chain reaction
title_short Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_full Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_fullStr Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_full_unstemmed Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
title_sort Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
author Marinheiro,Juliana Cristina
author_facet Marinheiro,Juliana Cristina
Sanalios,Roberta Braga
Santos,Daniela Carvalho dos
Costa,Cristovão Alves da
Hársi,Charlotte Marianna
author_role author
author2 Sanalios,Roberta Braga
Santos,Daniela Carvalho dos
Costa,Cristovão Alves da
Hársi,Charlotte Marianna
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Marinheiro,Juliana Cristina
Sanalios,Roberta Braga
Santos,Daniela Carvalho dos
Costa,Cristovão Alves da
Hársi,Charlotte Marianna
dc.subject.por.fl_str_mv human adenovirus
human respiratory syncytial virus
acute respiratory infection
polymerase chain reaction
topic human adenovirus
human respiratory syncytial virus
acute respiratory infection
polymerase chain reaction
dc.description.none.fl_txt_mv Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
description Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
publishDate 2009
dc.date.none.fl_str_mv 2009-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000100018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762009000100018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.104 n.1 2009
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937704301756416

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