Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

• Autor(a) principal: Marinheiro, Juliana Cristina
• Data de Publicação: 2009
• Outros Autores: Sanalios, Roberta Braga, dos Santos, Daniela Carvalho, Costa, Cristóvão Alves da, Hársi, Charlotte Marianna
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/14517
• topic: Adenoviridae; Human Adenovirus; Human Respiratory Syncytial Virus; Hydrangea Ringspot Virus; Respiratory Syncytial Virus; Adenoviridae; Human Adenovirus; Human Respiratory Syncytial Virus; Hydrangea Ringspot Virus; Respiratory Syncytial Virus; Acute Disease; Adenovirus; Adenovirus Infection; Case Control Study; Child; Genetics; Human; Isolation And Purification; Methodology; Nasopharynx; Polymerase Chain Reaction; Respiratory Syncytial Pneumovirus; Respiratory Tract Infection; Sensitivity And Specificity; Virology; Virus Infection; Acute Disease; Adenoviridae; Adenoviridae Infections; Case-control Studies; Child; Humans; Nasopharynx; Polymerase Chain Reaction; Respiratory Syncytial Virus Infections; Human Respiratory Syncytial Virus; Respiratory Tract Infections; Sensitivity And Specificity

Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used: 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.