Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1

Detalhes bibliográficos
Autor(a) principal: Cândido,AL
Data de Publicação: 2000
Outros Autores: Bontempo,ED, Resende,M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015
Resumo: Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
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spelling Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1bovine herpesvirus-1cosolventspolymerase chain reactionEspecial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.Instituto Oswaldo Cruz, Ministério da Saúde2000-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015Memórias do Instituto Oswaldo Cruz v.95 n.2 2000reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762000000200015info:eu-repo/semantics/openAccessCândido,ALBontempo,EDResende,Meng2020-04-25T17:48:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:09:36.091Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
title Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
spellingShingle Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
Cândido,AL
bovine herpesvirus-1
cosolvents
polymerase chain reaction
title_short Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
title_full Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
title_fullStr Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
title_full_unstemmed Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
title_sort Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
author Cândido,AL
author_facet Cândido,AL
Bontempo,ED
Resende,M
author_role author
author2 Bontempo,ED
Resende,M
author2_role author
author
dc.contributor.author.fl_str_mv Cândido,AL
Bontempo,ED
Resende,M
dc.subject.por.fl_str_mv bovine herpesvirus-1
cosolvents
polymerase chain reaction
topic bovine herpesvirus-1
cosolvents
polymerase chain reaction
dc.description.none.fl_txt_mv Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
description Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
publishDate 2000
dc.date.none.fl_str_mv 2000-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762000000200015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.95 n.2 2000
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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