Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1

Detalhes bibliográficos
Autor(a) principal: Cândido, Alexandre Luna
Data de Publicação: 2010
Outros Autores: Bontempo, Elizabete Dias, Resende, Maurício
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFS
Texto Completo: https://ri.ufs.br/handle/riufs/865
Resumo: Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
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spelling Cândido, Alexandre LunaBontempo, Elizabete DiasResende, Maurício2014-02-03T19:22:58Z2014-02-03T19:22:58Z2010-04CANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.0074-0276https://ri.ufs.br/handle/riufs/865Creative Commons Attribution LicenseEspecial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.Fundação Oswaldo CruzHerpesvirus bovinoCo-solventesDNAReação em cadeia da polimerasePolymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILPolymeraseChainReaction.pdf.jpgPolymeraseChainReaction.pdf.jpgGenerated Thumbnailimage/jpeg1757https://ri.ufs.br/jspui/bitstream/riufs/865/4/PolymeraseChainReaction.pdf.jpge0da8767fb6bb1773bbc947f9440a207MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/865/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALPolymeraseChainReaction.pdfPolymeraseChainReaction.pdfapplication/pdf46350https://ri.ufs.br/jspui/bitstream/riufs/865/1/PolymeraseChainReaction.pdf30f574a412b17d5c1c0412d609f73aa8MD51TEXTPolymeraseChainReaction.pdf.txtPolymeraseChainReaction.pdf.txtExtracted texttext/plain5960https://ri.ufs.br/jspui/bitstream/riufs/865/3/PolymeraseChainReaction.pdf.txtf1c2db20dd9e48987730ec353c7a80bdMD53riufs/8652014-02-04 02:00:17.177oai:ufs.br:riufs/865Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2014-02-04T05:00:17Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
title Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
spellingShingle Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
Cândido, Alexandre Luna
Herpesvirus bovino
Co-solventes
DNA
Reação em cadeia da polimerase
title_short Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
title_full Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
title_fullStr Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
title_full_unstemmed Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
title_sort Polymerase Chain Reaction Amplification of Genomic Fragments of Bovine Herpesvirus-1
author Cândido, Alexandre Luna
author_facet Cândido, Alexandre Luna
Bontempo, Elizabete Dias
Resende, Maurício
author_role author
author2 Bontempo, Elizabete Dias
Resende, Maurício
author2_role author
author
dc.contributor.author.fl_str_mv Cândido, Alexandre Luna
Bontempo, Elizabete Dias
Resende, Maurício
dc.subject.por.fl_str_mv Herpesvirus bovino
Co-solventes
DNA
Reação em cadeia da polimerase
topic Herpesvirus bovino
Co-solventes
DNA
Reação em cadeia da polimerase
description Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
publishDate 2010
dc.date.issued.fl_str_mv 2010-04
dc.date.accessioned.fl_str_mv 2014-02-03T19:22:58Z
dc.date.available.fl_str_mv 2014-02-03T19:22:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.
dc.identifier.uri.fl_str_mv https://ri.ufs.br/handle/riufs/865
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.license.pt_BR.fl_str_mv Creative Commons Attribution License
identifier_str_mv CANDIDO, A. L.; BONTEMPO, E. D.; RESENDE, M. Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 2, abr. 2000. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.
0074-0276
Creative Commons Attribution License
url https://ri.ufs.br/handle/riufs/865
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
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