The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient

Detalhes bibliográficos
Autor(a) principal: Silva,Luciana Diniz
Data de Publicação: 2011
Outros Autores: Rocha,Andreia Maria Camargos, Rocha,Gifone Aguiar, Moura,Sílvia Beleza de, Rocha,Márcia Maria Negreiros Pinto, Dani,Renato, Melo,Fabrício Freire de, Guerra,Juliana Becattini, Castro,Lúcia Porto Fonseca de, Mendes,Guilherme Santiago, Ferrari,Teresa Cristina de Abreu, Lima,Agnaldo Soares, Queiroz,Dulciene Maria Magalhães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600016
Resumo: The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.
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spelling The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patientHelicobacter pyloriliver diseasesIL-10IL-17IFN-γThe hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.Instituto Oswaldo Cruz, Ministério da Saúde2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600016Memórias do Instituto Oswaldo Cruz v.106 n.6 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000600016info:eu-repo/semantics/openAccessSilva,Luciana DinizRocha,Andreia Maria CamargosRocha,Gifone AguiarMoura,Sílvia Beleza deRocha,Márcia Maria Negreiros PintoDani,RenatoMelo,Fabrício Freire deGuerra,Juliana BecattiniCastro,Lúcia Porto Fonseca deMendes,Guilherme SantiagoFerrari,Teresa Cristina de AbreuLima,Agnaldo SoaresQueiroz,Dulciene Maria Magalhãeseng2020-04-25T17:51:04Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:50.824Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
title The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
spellingShingle The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
Silva,Luciana Diniz
Helicobacter pylori
liver diseases
IL-10
IL-17
IFN-γ
title_short The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
title_full The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
title_fullStr The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
title_full_unstemmed The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
title_sort The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient
author Silva,Luciana Diniz
author_facet Silva,Luciana Diniz
Rocha,Andreia Maria Camargos
Rocha,Gifone Aguiar
Moura,Sílvia Beleza de
Rocha,Márcia Maria Negreiros Pinto
Dani,Renato
Melo,Fabrício Freire de
Guerra,Juliana Becattini
Castro,Lúcia Porto Fonseca de
Mendes,Guilherme Santiago
Ferrari,Teresa Cristina de Abreu
Lima,Agnaldo Soares
Queiroz,Dulciene Maria Magalhães
author_role author
author2 Rocha,Andreia Maria Camargos
Rocha,Gifone Aguiar
Moura,Sílvia Beleza de
Rocha,Márcia Maria Negreiros Pinto
Dani,Renato
Melo,Fabrício Freire de
Guerra,Juliana Becattini
Castro,Lúcia Porto Fonseca de
Mendes,Guilherme Santiago
Ferrari,Teresa Cristina de Abreu
Lima,Agnaldo Soares
Queiroz,Dulciene Maria Magalhães
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Luciana Diniz
Rocha,Andreia Maria Camargos
Rocha,Gifone Aguiar
Moura,Sílvia Beleza de
Rocha,Márcia Maria Negreiros Pinto
Dani,Renato
Melo,Fabrício Freire de
Guerra,Juliana Becattini
Castro,Lúcia Porto Fonseca de
Mendes,Guilherme Santiago
Ferrari,Teresa Cristina de Abreu
Lima,Agnaldo Soares
Queiroz,Dulciene Maria Magalhães
dc.subject.por.fl_str_mv Helicobacter pylori
liver diseases
IL-10
IL-17
IFN-γ
topic Helicobacter pylori
liver diseases
IL-10
IL-17
IFN-γ
dc.description.none.fl_txt_mv The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.
description The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600016
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000600016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000600016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.6 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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