Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Santos,Dilvani O.
Data de Publicação: 1988
Outros Autores: Oliveira,Mécia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004
Resumo: Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active.
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spelling Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzimacromolecule synthesisradioactive tracersautoradiographic studiescAMPDibutyryl-cAMPisoproterenolMacromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active.Instituto Oswaldo Cruz, Ministério da Saúde1988-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004Memórias do Instituto Oswaldo Cruz v.83 n.3 1988reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761988000300004info:eu-repo/semantics/openAccessSantos,Dilvani O.Oliveira,Mécia M.eng2020-04-25T17:46:06Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:26.871Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
title Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
spellingShingle Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
Santos,Dilvani O.
macromolecule synthesis
radioactive tracers
autoradiographic studies
cAMP
Dibutyryl-cAMP
isoproterenol
title_short Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
title_full Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
title_fullStr Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
title_full_unstemmed Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
title_sort Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
author Santos,Dilvani O.
author_facet Santos,Dilvani O.
Oliveira,Mécia M.
author_role author
author2 Oliveira,Mécia M.
author2_role author
dc.contributor.author.fl_str_mv Santos,Dilvani O.
Oliveira,Mécia M.
dc.subject.por.fl_str_mv macromolecule synthesis
radioactive tracers
autoradiographic studies
cAMP
Dibutyryl-cAMP
isoproterenol
topic macromolecule synthesis
radioactive tracers
autoradiographic studies
cAMP
Dibutyryl-cAMP
isoproterenol
dc.description.none.fl_txt_mv Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active.
description Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active.
publishDate 1988
dc.date.none.fl_str_mv 1988-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761988000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.83 n.3 1988
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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