Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004 |
Resumo: | Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active. |
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Memórias do Instituto Oswaldo Cruz |
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Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzimacromolecule synthesisradioactive tracersautoradiographic studiescAMPDibutyryl-cAMPisoproterenolMacromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active.Instituto Oswaldo Cruz, Ministério da Saúde1988-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004Memórias do Instituto Oswaldo Cruz v.83 n.3 1988reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761988000300004info:eu-repo/semantics/openAccessSantos,Dilvani O.Oliveira,Mécia M.eng2020-04-25T17:46:06Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:26.871Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
title |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
spellingShingle |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi Santos,Dilvani O. macromolecule synthesis radioactive tracers autoradiographic studies cAMP Dibutyryl-cAMP isoproterenol |
title_short |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
title_full |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
title_fullStr |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
title_full_unstemmed |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
title_sort |
Effect of cAMP on macromolecule synthesis in the pathogenic protozoa Trypanosoma cruzi |
author |
Santos,Dilvani O. |
author_facet |
Santos,Dilvani O. Oliveira,Mécia M. |
author_role |
author |
author2 |
Oliveira,Mécia M. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Santos,Dilvani O. Oliveira,Mécia M. |
dc.subject.por.fl_str_mv |
macromolecule synthesis radioactive tracers autoradiographic studies cAMP Dibutyryl-cAMP isoproterenol |
topic |
macromolecule synthesis radioactive tracers autoradiographic studies cAMP Dibutyryl-cAMP isoproterenol |
dc.description.none.fl_txt_mv |
Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active. |
description |
Macromolecule synthesis of Trypanosoma cruzi in culture was monitored using radioactive tracers. Cells of different days in culture displayed a preferential incorporation of precursors as follows: 1 day for (³H)-thymidine cells; 3 days for (³H)-uridine cells, and 4 days for (³H)-leucine cells. Autoradiographic studies showed that (³H)-thymidine was incorporated in the DNA of both kinetoplast and nucleus in this order. Shifts in the intracellular content of cAMP either by addition of dibutyryl-cAMP or by stimulation of the adenylcyclase by isoproterenol, caused an inhibition in the synthesis of DNA, RNA and proteins. Addition to the T. cruzi cultures of these agents which elevate the intracellular content ofcAMP provoked an interruption of cell proliferation as a result of the impairment of macromolecule synthesis. A discrimination was observed among the stereoisomers of isoproterenol, the L configuration showing to be most active. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000300004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761988000300004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.83 n.3 1988 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937651074990080 |