Molecular aspects of Schistosoma mansoni female maturation

Detalhes bibliográficos
Autor(a) principal: Giannini,Ana Lúcia Moraes
Data de Publicação: 1995
Outros Autores: Linhares,Sérgio Vasconcelos, Caride,Elena Cristina, Braga,Vânia Maria Martins, Rumjanek,Franklin David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200009
Resumo: Incubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.
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spelling Molecular aspects of Schistosoma mansoni female maturationSchistosoma mansoninuclear proteinsF-10 genesteroid receptorsTamoxifeneggsIncubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.Instituto Oswaldo Cruz, Ministério da Saúde1995-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200009Memórias do Instituto Oswaldo Cruz v.90 n.2 1995reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761995000200009info:eu-repo/semantics/openAccessGiannini,Ana Lúcia MoraesLinhares,Sérgio VasconcelosCaride,Elena CristinaBraga,Vânia Maria MartinsRumjanek,Franklin Davideng2020-04-25T17:47:23Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:26.295Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular aspects of Schistosoma mansoni female maturation
title Molecular aspects of Schistosoma mansoni female maturation
spellingShingle Molecular aspects of Schistosoma mansoni female maturation
Giannini,Ana Lúcia Moraes
Schistosoma mansoni
nuclear proteins
F-10 gene
steroid receptors
Tamoxifen
eggs
title_short Molecular aspects of Schistosoma mansoni female maturation
title_full Molecular aspects of Schistosoma mansoni female maturation
title_fullStr Molecular aspects of Schistosoma mansoni female maturation
title_full_unstemmed Molecular aspects of Schistosoma mansoni female maturation
title_sort Molecular aspects of Schistosoma mansoni female maturation
author Giannini,Ana Lúcia Moraes
author_facet Giannini,Ana Lúcia Moraes
Linhares,Sérgio Vasconcelos
Caride,Elena Cristina
Braga,Vânia Maria Martins
Rumjanek,Franklin David
author_role author
author2 Linhares,Sérgio Vasconcelos
Caride,Elena Cristina
Braga,Vânia Maria Martins
Rumjanek,Franklin David
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Giannini,Ana Lúcia Moraes
Linhares,Sérgio Vasconcelos
Caride,Elena Cristina
Braga,Vânia Maria Martins
Rumjanek,Franklin David
dc.subject.por.fl_str_mv Schistosoma mansoni
nuclear proteins
F-10 gene
steroid receptors
Tamoxifen
eggs
topic Schistosoma mansoni
nuclear proteins
F-10 gene
steroid receptors
Tamoxifen
eggs
dc.description.none.fl_txt_mv Incubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.
description Incubation of total protein extracts of Schistosoma mansoni with 3H 17-beta-estradiol and 20-hydroxyecdysone, revealed steroid binding proteins in both, male and female worms. The interaction of nuclear proteins with restriction fragments of the gender and stage-specific gene F-10 was investigated using the "Band-Shift" technique. Distinct male and female nuclear proteins bound to the fragments of this gene. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the estrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females, a band profile similar to that obtained with male worm protein extracts. When Tamoxifen was injected into schistosome infected mice, the eggs produced by females presented an abnormal morphology, compatible with non-viable eggs. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors.
publishDate 1995
dc.date.none.fl_str_mv 1995-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761995000200009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.90 n.2 1995
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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