Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens

Detalhes bibliográficos
Autor(a) principal: Caterino-de-Araujo,Adele
Data de Publicação: 1992
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200012
Resumo: The present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.
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spelling Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigenshuman immunodeficiency virus type 1 (HIV-1)in vitro secretion of HIV-1-specific antibodydiagnosis of pediatric HIV-1 infectionThe present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.Instituto Oswaldo Cruz, Ministério da Saúde1992-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200012Memórias do Instituto Oswaldo Cruz v.87 n.2 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000200012info:eu-repo/semantics/openAccessCaterino-de-Araujo,Adeleeng2020-04-25T17:46:45Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:36.556Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
title Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
spellingShingle Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
Caterino-de-Araujo,Adele
human immunodeficiency virus type 1 (HIV-1)
in vitro secretion of HIV-1-specific antibody
diagnosis of pediatric HIV-1 infection
title_short Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
title_full Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
title_fullStr Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
title_full_unstemmed Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
title_sort Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
author Caterino-de-Araujo,Adele
author_facet Caterino-de-Araujo,Adele
author_role author
dc.contributor.author.fl_str_mv Caterino-de-Araujo,Adele
dc.subject.por.fl_str_mv human immunodeficiency virus type 1 (HIV-1)
in vitro secretion of HIV-1-specific antibody
diagnosis of pediatric HIV-1 infection
topic human immunodeficiency virus type 1 (HIV-1)
in vitro secretion of HIV-1-specific antibody
diagnosis of pediatric HIV-1 infection
dc.description.none.fl_txt_mv The present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.
description The present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection.
publishDate 1992
dc.date.none.fl_str_mv 1992-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200012
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000200012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 n.2 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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