Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500004 |
Resumo: | The epidemiologic typing of bacterial pathogens can be applied to answer a number of different questions: in case of outbreak, what is the extent and mode of transmission of epidemic clone(s )? In case of long-term surveillance, what is the prevalence over time and the geographic spread of epidemic and endemic clones in the population? A number of molecular typing methods can be used to classify bacteria based on genomic diversity into groups of closely-related isolates (presumed to arise from a common ancestor in the same chain of transmission) and divergent, epidemiologically-unrelated isolates (arising from independent sources of infection). Ribotyping, IS-RFLP fingerprinting, macrorestriction analysis of chromosomal DNA and PCR-fingerprinting using arbitrary sequence or repeat element primers are useful methods for outbreak investigations and regional surveillance. Library typing systems based on multilocus sequence-based analysis and strain-specific probe hybridization schemes are in development for the international surveillance of major pathogens like Mycobacterium tuberculosis. Accurate epidemiological interpretation of data obtained with molecular typing systems still requires additional research on the evolution rate of polymorphic loci in bacterial pathogens. |
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Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectivesbacterial typingDNA polymorphismpulsed-field gel electrophoresisribotypingRFLP typingPCR fingerprintingrandomly amplified polymorphic DNA (RAPD)crossinfectionepidemiologysurveillanceThe epidemiologic typing of bacterial pathogens can be applied to answer a number of different questions: in case of outbreak, what is the extent and mode of transmission of epidemic clone(s )? In case of long-term surveillance, what is the prevalence over time and the geographic spread of epidemic and endemic clones in the population? A number of molecular typing methods can be used to classify bacteria based on genomic diversity into groups of closely-related isolates (presumed to arise from a common ancestor in the same chain of transmission) and divergent, epidemiologically-unrelated isolates (arising from independent sources of infection). Ribotyping, IS-RFLP fingerprinting, macrorestriction analysis of chromosomal DNA and PCR-fingerprinting using arbitrary sequence or repeat element primers are useful methods for outbreak investigations and regional surveillance. Library typing systems based on multilocus sequence-based analysis and strain-specific probe hybridization schemes are in development for the international surveillance of major pathogens like Mycobacterium tuberculosis. Accurate epidemiological interpretation of data obtained with molecular typing systems still requires additional research on the evolution rate of polymorphic loci in bacterial pathogens.Instituto Oswaldo Cruz, Ministério da Saúde1998-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500004Memórias do Instituto Oswaldo Cruz v.93 n.5 1998reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761998000500004info:eu-repo/semantics/openAccessStruelens,Marc Jeng2020-04-25T17:47:54Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:08:14.863Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
title |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
spellingShingle |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives Struelens,Marc J bacterial typing DNA polymorphism pulsed-field gel electrophoresis ribotyping RFLP typing PCR fingerprinting randomly amplified polymorphic DNA (RAPD) cross infection epidemiology surveillance |
title_short |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
title_full |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
title_fullStr |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
title_full_unstemmed |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
title_sort |
Molecular Epidemiologic Typing Systems of Bacterial Pathogens: Current Issues and Perpectives |
author |
Struelens,Marc J |
author_facet |
Struelens,Marc J |
author_role |
author |
dc.contributor.author.fl_str_mv |
Struelens,Marc J |
dc.subject.por.fl_str_mv |
bacterial typing DNA polymorphism pulsed-field gel electrophoresis ribotyping RFLP typing PCR fingerprinting randomly amplified polymorphic DNA (RAPD) cross infection epidemiology surveillance |
topic |
bacterial typing DNA polymorphism pulsed-field gel electrophoresis ribotyping RFLP typing PCR fingerprinting randomly amplified polymorphic DNA (RAPD) cross infection epidemiology surveillance |
dc.description.none.fl_txt_mv |
The epidemiologic typing of bacterial pathogens can be applied to answer a number of different questions: in case of outbreak, what is the extent and mode of transmission of epidemic clone(s )? In case of long-term surveillance, what is the prevalence over time and the geographic spread of epidemic and endemic clones in the population? A number of molecular typing methods can be used to classify bacteria based on genomic diversity into groups of closely-related isolates (presumed to arise from a common ancestor in the same chain of transmission) and divergent, epidemiologically-unrelated isolates (arising from independent sources of infection). Ribotyping, IS-RFLP fingerprinting, macrorestriction analysis of chromosomal DNA and PCR-fingerprinting using arbitrary sequence or repeat element primers are useful methods for outbreak investigations and regional surveillance. Library typing systems based on multilocus sequence-based analysis and strain-specific probe hybridization schemes are in development for the international surveillance of major pathogens like Mycobacterium tuberculosis. Accurate epidemiological interpretation of data obtained with molecular typing systems still requires additional research on the evolution rate of polymorphic loci in bacterial pathogens. |
description |
The epidemiologic typing of bacterial pathogens can be applied to answer a number of different questions: in case of outbreak, what is the extent and mode of transmission of epidemic clone(s )? In case of long-term surveillance, what is the prevalence over time and the geographic spread of epidemic and endemic clones in the population? A number of molecular typing methods can be used to classify bacteria based on genomic diversity into groups of closely-related isolates (presumed to arise from a common ancestor in the same chain of transmission) and divergent, epidemiologically-unrelated isolates (arising from independent sources of infection). Ribotyping, IS-RFLP fingerprinting, macrorestriction analysis of chromosomal DNA and PCR-fingerprinting using arbitrary sequence or repeat element primers are useful methods for outbreak investigations and regional surveillance. Library typing systems based on multilocus sequence-based analysis and strain-specific probe hybridization schemes are in development for the international surveillance of major pathogens like Mycobacterium tuberculosis. Accurate epidemiological interpretation of data obtained with molecular typing systems still requires additional research on the evolution rate of polymorphic loci in bacterial pathogens. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500004 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761998000500004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.93 n.5 1998 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937673048948736 |