Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018 |
Resumo: | The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains. |
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Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strainsCorynebacterium diphtheriaediphtheriafibrinfibrinogenpseudomembranenontoxigenicThe production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains.Instituto Oswaldo Cruz, Ministério da Saúde2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018Memórias do Instituto Oswaldo Cruz v.105 n.5 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000500018info:eu-repo/semantics/openAccessSabbadini,Priscila SoaresGenovez,Marcia Rocha NovaisSilva,Cecília Ferreira daAdelino,Thelma Lúcia NovaesSantos,Cintia Silva dosPereira,Gabriela AndradeNagao,Prescilla EmyDias,Alexandre Alves de Souza de OliveiraMattos-Guaraldi,Ana LuizaHirata Júnior,Raphaeleng2020-04-25T17:50:50Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:12.32Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
title |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
spellingShingle |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains Sabbadini,Priscila Soares Corynebacterium diphtheriae diphtheria fibrin fibrinogen pseudomembrane nontoxigenic |
title_short |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
title_full |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
title_fullStr |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
title_full_unstemmed |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
title_sort |
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains |
author |
Sabbadini,Priscila Soares |
author_facet |
Sabbadini,Priscila Soares Genovez,Marcia Rocha Novais Silva,Cecília Ferreira da Adelino,Thelma Lúcia Novaes Santos,Cintia Silva dos Pereira,Gabriela Andrade Nagao,Prescilla Emy Dias,Alexandre Alves de Souza de Oliveira Mattos-Guaraldi,Ana Luiza Hirata Júnior,Raphael |
author_role |
author |
author2 |
Genovez,Marcia Rocha Novais Silva,Cecília Ferreira da Adelino,Thelma Lúcia Novaes Santos,Cintia Silva dos Pereira,Gabriela Andrade Nagao,Prescilla Emy Dias,Alexandre Alves de Souza de Oliveira Mattos-Guaraldi,Ana Luiza Hirata Júnior,Raphael |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sabbadini,Priscila Soares Genovez,Marcia Rocha Novais Silva,Cecília Ferreira da Adelino,Thelma Lúcia Novaes Santos,Cintia Silva dos Pereira,Gabriela Andrade Nagao,Prescilla Emy Dias,Alexandre Alves de Souza de Oliveira Mattos-Guaraldi,Ana Luiza Hirata Júnior,Raphael |
dc.subject.por.fl_str_mv |
Corynebacterium diphtheriae diphtheria fibrin fibrinogen pseudomembrane nontoxigenic |
topic |
Corynebacterium diphtheriae diphtheria fibrin fibrinogen pseudomembrane nontoxigenic |
dc.description.none.fl_txt_mv |
The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains. |
description |
The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762010000500018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.105 n.5 2010 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937707985403904 |