Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains

Detalhes bibliográficos
Autor(a) principal: Sabbadini,Priscila Soares
Data de Publicação: 2010
Outros Autores: Genovez,Marcia Rocha Novais, Silva,Cecília Ferreira da, Adelino,Thelma Lúcia Novaes, Santos,Cintia Silva dos, Pereira,Gabriela Andrade, Nagao,Prescilla Emy, Dias,Alexandre Alves de Souza de Oliveira, Mattos-Guaraldi,Ana Luiza, Hirata Júnior,Raphael
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018
Resumo: The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains.
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spelling Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strainsCorynebacterium diphtheriaediphtheriafibrinfibrinogenpseudomembranenontoxigenicThe production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains.Instituto Oswaldo Cruz, Ministério da Saúde2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018Memórias do Instituto Oswaldo Cruz v.105 n.5 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000500018info:eu-repo/semantics/openAccessSabbadini,Priscila SoaresGenovez,Marcia Rocha NovaisSilva,Cecília Ferreira daAdelino,Thelma Lúcia NovaesSantos,Cintia Silva dosPereira,Gabriela AndradeNagao,Prescilla EmyDias,Alexandre Alves de Souza de OliveiraMattos-Guaraldi,Ana LuizaHirata Júnior,Raphaeleng2020-04-25T17:50:50Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:12.32Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
title Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
spellingShingle Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
Sabbadini,Priscila Soares
Corynebacterium diphtheriae
diphtheria
fibrin
fibrinogen
pseudomembrane
nontoxigenic
title_short Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
title_full Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
title_fullStr Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
title_full_unstemmed Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
title_sort Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
author Sabbadini,Priscila Soares
author_facet Sabbadini,Priscila Soares
Genovez,Marcia Rocha Novais
Silva,Cecília Ferreira da
Adelino,Thelma Lúcia Novaes
Santos,Cintia Silva dos
Pereira,Gabriela Andrade
Nagao,Prescilla Emy
Dias,Alexandre Alves de Souza de Oliveira
Mattos-Guaraldi,Ana Luiza
Hirata Júnior,Raphael
author_role author
author2 Genovez,Marcia Rocha Novais
Silva,Cecília Ferreira da
Adelino,Thelma Lúcia Novaes
Santos,Cintia Silva dos
Pereira,Gabriela Andrade
Nagao,Prescilla Emy
Dias,Alexandre Alves de Souza de Oliveira
Mattos-Guaraldi,Ana Luiza
Hirata Júnior,Raphael
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sabbadini,Priscila Soares
Genovez,Marcia Rocha Novais
Silva,Cecília Ferreira da
Adelino,Thelma Lúcia Novaes
Santos,Cintia Silva dos
Pereira,Gabriela Andrade
Nagao,Prescilla Emy
Dias,Alexandre Alves de Souza de Oliveira
Mattos-Guaraldi,Ana Luiza
Hirata Júnior,Raphael
dc.subject.por.fl_str_mv Corynebacterium diphtheriae
diphtheria
fibrin
fibrinogen
pseudomembrane
nontoxigenic
topic Corynebacterium diphtheriae
diphtheria
fibrin
fibrinogen
pseudomembrane
nontoxigenic
dc.description.none.fl_txt_mv The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains.
description The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000500018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.5 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
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repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
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