Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Macedo,Andrea M
Data de Publicação: 2001
Outros Autores: Pimenta,Juliana R, Aguiar,Renato S de, Melo,Anna Izabel R, Chiari,Egler, Zingales,Bianca, Pena,Sérgio DJ, Oliveira,Riva P
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000300023
Resumo: Through microsatellite analysis of 53 monoclonal populations of Trypanosoma cruzi, we found a remarkable degree of genetic polymorphism with no single multilocus genotype being observed more than once. The microsatellite profile proved to be stable during 70 generations of the CL Brener clone in culture. The microsatellite profiling presented also high diagnostic sensitivity since DNA amplifications could be achieved with less than 100 fg DNA, corresponding to half parasite total DNA content. Based on these technical attributes the microsatellite assay turns out to be an important tool for direct typing T. cruzi in biological samples. By using this approach we were able to type T. cruzi in feces of artificially infected bugs and in single cells sorted by FACS. The microsatellites have shown to be excellent markers for T. cruzi phylogenetic reconstruction. We used maximum parsimony based on the minimum number of mutational steps to build an unrooted Wagner network, which confirms previous conclusions based on the analysis of the D7 domain of the LSU rDNA gene that T. cruzi is composed by two major groups. We also obtained evidence that strains belonging to rRNA group 2 are subdivided into two genetically distant clusters, and that one of these clusters is more related to rRNA group 1/2. These results suggest different origins for these strains.
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spelling Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruziTrypanosoma cruzimicrosatellitesgenetic typingrDNA classificationphylogenetic inferenceThrough microsatellite analysis of 53 monoclonal populations of Trypanosoma cruzi, we found a remarkable degree of genetic polymorphism with no single multilocus genotype being observed more than once. The microsatellite profile proved to be stable during 70 generations of the CL Brener clone in culture. The microsatellite profiling presented also high diagnostic sensitivity since DNA amplifications could be achieved with less than 100 fg DNA, corresponding to half parasite total DNA content. Based on these technical attributes the microsatellite assay turns out to be an important tool for direct typing T. cruzi in biological samples. By using this approach we were able to type T. cruzi in feces of artificially infected bugs and in single cells sorted by FACS. The microsatellites have shown to be excellent markers for T. cruzi phylogenetic reconstruction. We used maximum parsimony based on the minimum number of mutational steps to build an unrooted Wagner network, which confirms previous conclusions based on the analysis of the D7 domain of the LSU rDNA gene that T. cruzi is composed by two major groups. We also obtained evidence that strains belonging to rRNA group 2 are subdivided into two genetically distant clusters, and that one of these clusters is more related to rRNA group 1/2. These results suggest different origins for these strains.Instituto Oswaldo Cruz, Ministério da Saúde2001-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000300023Memórias do Instituto Oswaldo Cruz v.96 n.3 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000300023info:eu-repo/semantics/openAccessMacedo,Andrea MPimenta,Juliana RAguiar,Renato S deMelo,Anna Izabel RChiari,EglerZingales,BiancaPena,Sérgio DJOliveira,Riva Peng2020-04-25T17:48:33Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:17.006Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
title Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
spellingShingle Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
Macedo,Andrea M
Trypanosoma cruzi
microsatellites
genetic typing
rDNA classification
phylogenetic inference
title_short Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
title_full Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
title_fullStr Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
title_full_unstemmed Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
title_sort Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi
author Macedo,Andrea M
author_facet Macedo,Andrea M
Pimenta,Juliana R
Aguiar,Renato S de
Melo,Anna Izabel R
Chiari,Egler
Zingales,Bianca
Pena,Sérgio DJ
Oliveira,Riva P
author_role author
author2 Pimenta,Juliana R
Aguiar,Renato S de
Melo,Anna Izabel R
Chiari,Egler
Zingales,Bianca
Pena,Sérgio DJ
Oliveira,Riva P
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Macedo,Andrea M
Pimenta,Juliana R
Aguiar,Renato S de
Melo,Anna Izabel R
Chiari,Egler
Zingales,Bianca
Pena,Sérgio DJ
Oliveira,Riva P
dc.subject.por.fl_str_mv Trypanosoma cruzi
microsatellites
genetic typing
rDNA classification
phylogenetic inference
topic Trypanosoma cruzi
microsatellites
genetic typing
rDNA classification
phylogenetic inference
dc.description.none.fl_txt_mv Through microsatellite analysis of 53 monoclonal populations of Trypanosoma cruzi, we found a remarkable degree of genetic polymorphism with no single multilocus genotype being observed more than once. The microsatellite profile proved to be stable during 70 generations of the CL Brener clone in culture. The microsatellite profiling presented also high diagnostic sensitivity since DNA amplifications could be achieved with less than 100 fg DNA, corresponding to half parasite total DNA content. Based on these technical attributes the microsatellite assay turns out to be an important tool for direct typing T. cruzi in biological samples. By using this approach we were able to type T. cruzi in feces of artificially infected bugs and in single cells sorted by FACS. The microsatellites have shown to be excellent markers for T. cruzi phylogenetic reconstruction. We used maximum parsimony based on the minimum number of mutational steps to build an unrooted Wagner network, which confirms previous conclusions based on the analysis of the D7 domain of the LSU rDNA gene that T. cruzi is composed by two major groups. We also obtained evidence that strains belonging to rRNA group 2 are subdivided into two genetically distant clusters, and that one of these clusters is more related to rRNA group 1/2. These results suggest different origins for these strains.
description Through microsatellite analysis of 53 monoclonal populations of Trypanosoma cruzi, we found a remarkable degree of genetic polymorphism with no single multilocus genotype being observed more than once. The microsatellite profile proved to be stable during 70 generations of the CL Brener clone in culture. The microsatellite profiling presented also high diagnostic sensitivity since DNA amplifications could be achieved with less than 100 fg DNA, corresponding to half parasite total DNA content. Based on these technical attributes the microsatellite assay turns out to be an important tool for direct typing T. cruzi in biological samples. By using this approach we were able to type T. cruzi in feces of artificially infected bugs and in single cells sorted by FACS. The microsatellites have shown to be excellent markers for T. cruzi phylogenetic reconstruction. We used maximum parsimony based on the minimum number of mutational steps to build an unrooted Wagner network, which confirms previous conclusions based on the analysis of the D7 domain of the LSU rDNA gene that T. cruzi is composed by two major groups. We also obtained evidence that strains belonging to rRNA group 2 are subdivided into two genetically distant clusters, and that one of these clusters is more related to rRNA group 1/2. These results suggest different origins for these strains.
publishDate 2001
dc.date.none.fl_str_mv 2001-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000300023
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000300023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762001000300023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.96 n.3 2001
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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