Development of nuclear DNA probes for the typing of Trypanososma cruzi

Detalhes bibliográficos
Autor(a) principal: Takeuchi,A. Mayumi
Data de Publicação: 1991
Outros Autores: Traub-Cseko,Yara M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005
Resumo: We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.
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spelling Development of nuclear DNA probes for the typing of Trypanososma cruziTrypanosoma cruzirandom probestypingstrainsnumerical taxonomyWe have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level. Instituto Oswaldo Cruz, Ministério da Saúde1991-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005Memórias do Instituto Oswaldo Cruz v.86 n.3 1991reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761991000300005info:eu-repo/semantics/openAccessTakeuchi,A. MayumiTraub-Cseko,Yara M.eng2020-04-25T17:46:35Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:04.464Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Development of nuclear DNA probes for the typing of Trypanososma cruzi
title Development of nuclear DNA probes for the typing of Trypanososma cruzi
spellingShingle Development of nuclear DNA probes for the typing of Trypanososma cruzi
Takeuchi,A. Mayumi
Trypanosoma cruzi
random probes
typing
strains
numerical taxonomy
title_short Development of nuclear DNA probes for the typing of Trypanososma cruzi
title_full Development of nuclear DNA probes for the typing of Trypanososma cruzi
title_fullStr Development of nuclear DNA probes for the typing of Trypanososma cruzi
title_full_unstemmed Development of nuclear DNA probes for the typing of Trypanososma cruzi
title_sort Development of nuclear DNA probes for the typing of Trypanososma cruzi
author Takeuchi,A. Mayumi
author_facet Takeuchi,A. Mayumi
Traub-Cseko,Yara M.
author_role author
author2 Traub-Cseko,Yara M.
author2_role author
dc.contributor.author.fl_str_mv Takeuchi,A. Mayumi
Traub-Cseko,Yara M.
dc.subject.por.fl_str_mv Trypanosoma cruzi
random probes
typing
strains
numerical taxonomy
topic Trypanosoma cruzi
random probes
typing
strains
numerical taxonomy
dc.description.none.fl_txt_mv We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.
description We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level.
publishDate 1991
dc.date.none.fl_str_mv 1991-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761991000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.86 n.3 1991
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
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reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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