Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein

Detalhes bibliográficos
Autor(a) principal: Souza,Paulo R Eleutério de
Data de Publicação: 2001
Outros Autores: Valadão,Analina F, Calzavara-Silva,Carlos E, Franco,Glória R, Morais Júnior,Marcos A de, Abath,Frederico GC
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900018
Resumo: The zinc finger motifs (Cys2His2) are found in several proteins playing a role in the regulation of transcripton. SmZF1, a Schistosoma mansoni gene encoding a zinc finger protein was initially isolated from an adult worm cDNA library, as a partial cDNA. The full sequence of the gene was obtained by subcloning and sequencing cDNA and genomic fragments. The collated gene sequence is 2181 nt and the complete cDNA sequence is 705 bp containing the full open reading frame of the gene. Analysis of the genome sequence revealed the presence of three introns interrupting the coding region. The open reading frame theoretically encodes a protein of 164 amino acids, with a calculated molecular mass of 18,667Da. The predicted protein contains three zinc finger motifs, usually present in transcription regulatory proteins. PCR amplification with specific primers for the gene allowed for the detection of the target in egg, cercariae, schistosomulum and adult worm cDNA libraries indicating the expression of the mRNA in these life cycle stages of S. mansoni. This pattern of expression suggests the gene plays a role in vital functions of different life cycle stages of the parasite. Future research will be directed to elucidate the functional role of SmZF1.
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spelling Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger proteinDNA binding proteingene cloningSchistosoma mansonizinc finger proteinThe zinc finger motifs (Cys2His2) are found in several proteins playing a role in the regulation of transcripton. SmZF1, a Schistosoma mansoni gene encoding a zinc finger protein was initially isolated from an adult worm cDNA library, as a partial cDNA. The full sequence of the gene was obtained by subcloning and sequencing cDNA and genomic fragments. The collated gene sequence is 2181 nt and the complete cDNA sequence is 705 bp containing the full open reading frame of the gene. Analysis of the genome sequence revealed the presence of three introns interrupting the coding region. The open reading frame theoretically encodes a protein of 164 amino acids, with a calculated molecular mass of 18,667Da. The predicted protein contains three zinc finger motifs, usually present in transcription regulatory proteins. PCR amplification with specific primers for the gene allowed for the detection of the target in egg, cercariae, schistosomulum and adult worm cDNA libraries indicating the expression of the mRNA in these life cycle stages of S. mansoni. This pattern of expression suggests the gene plays a role in vital functions of different life cycle stages of the parasite. Future research will be directed to elucidate the functional role of SmZF1.Instituto Oswaldo Cruz, Ministério da Saúde2001-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900018Memórias do Instituto Oswaldo Cruz v.96 suppl.0 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000900018info:eu-repo/semantics/openAccessSouza,Paulo R Eleutério deValadão,Analina FCalzavara-Silva,Carlos EFranco,Glória RMorais Júnior,Marcos A deAbath,Frederico GCeng2020-04-25T17:48:41Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:46.878Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
title Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
spellingShingle Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
Souza,Paulo R Eleutério de
DNA binding protein
gene cloning
Schistosoma mansoni
zinc finger protein
title_short Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
title_full Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
title_fullStr Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
title_full_unstemmed Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
title_sort Cloning and characterization of SmZF1, a gene encoding a Schistosoma mansoni zinc finger protein
author Souza,Paulo R Eleutério de
author_facet Souza,Paulo R Eleutério de
Valadão,Analina F
Calzavara-Silva,Carlos E
Franco,Glória R
Morais Júnior,Marcos A de
Abath,Frederico GC
author_role author
author2 Valadão,Analina F
Calzavara-Silva,Carlos E
Franco,Glória R
Morais Júnior,Marcos A de
Abath,Frederico GC
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza,Paulo R Eleutério de
Valadão,Analina F
Calzavara-Silva,Carlos E
Franco,Glória R
Morais Júnior,Marcos A de
Abath,Frederico GC
dc.subject.por.fl_str_mv DNA binding protein
gene cloning
Schistosoma mansoni
zinc finger protein
topic DNA binding protein
gene cloning
Schistosoma mansoni
zinc finger protein
dc.description.none.fl_txt_mv The zinc finger motifs (Cys2His2) are found in several proteins playing a role in the regulation of transcripton. SmZF1, a Schistosoma mansoni gene encoding a zinc finger protein was initially isolated from an adult worm cDNA library, as a partial cDNA. The full sequence of the gene was obtained by subcloning and sequencing cDNA and genomic fragments. The collated gene sequence is 2181 nt and the complete cDNA sequence is 705 bp containing the full open reading frame of the gene. Analysis of the genome sequence revealed the presence of three introns interrupting the coding region. The open reading frame theoretically encodes a protein of 164 amino acids, with a calculated molecular mass of 18,667Da. The predicted protein contains three zinc finger motifs, usually present in transcription regulatory proteins. PCR amplification with specific primers for the gene allowed for the detection of the target in egg, cercariae, schistosomulum and adult worm cDNA libraries indicating the expression of the mRNA in these life cycle stages of S. mansoni. This pattern of expression suggests the gene plays a role in vital functions of different life cycle stages of the parasite. Future research will be directed to elucidate the functional role of SmZF1.
description The zinc finger motifs (Cys2His2) are found in several proteins playing a role in the regulation of transcripton. SmZF1, a Schistosoma mansoni gene encoding a zinc finger protein was initially isolated from an adult worm cDNA library, as a partial cDNA. The full sequence of the gene was obtained by subcloning and sequencing cDNA and genomic fragments. The collated gene sequence is 2181 nt and the complete cDNA sequence is 705 bp containing the full open reading frame of the gene. Analysis of the genome sequence revealed the presence of three introns interrupting the coding region. The open reading frame theoretically encodes a protein of 164 amino acids, with a calculated molecular mass of 18,667Da. The predicted protein contains three zinc finger motifs, usually present in transcription regulatory proteins. PCR amplification with specific primers for the gene allowed for the detection of the target in egg, cercariae, schistosomulum and adult worm cDNA libraries indicating the expression of the mRNA in these life cycle stages of S. mansoni. This pattern of expression suggests the gene plays a role in vital functions of different life cycle stages of the parasite. Future research will be directed to elucidate the functional role of SmZF1.
publishDate 2001
dc.date.none.fl_str_mv 2001-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000900018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762001000900018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.96 suppl.0 2001
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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