On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Farias, Leonardo P.
Data de Publicação: 2013
Outros Autores: Peterson, Greice Krautz, Tararam, Cibele A., Montoya, Bogar O. Araujo, Fraga, Tatiana R., Rofatto, Henrique K., Silva Jr., Floriano P., Isaac, Lourdes, Da`dara, Akram A., Wilson, R. Alan, Shoemaker, Charles B., Leite, Luciana C. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11533
Resumo: Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil.
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spelling Farias, Leonardo P.Peterson, Greice KrautzTararam, Cibele A.Montoya, Bogar O. AraujoFraga, Tatiana R.Rofatto, Henrique K.Silva Jr., Floriano P.Isaac, LourdesDa`dara, Akram A.Wilson, R. AlanShoemaker, Charles B.Leite, Luciana C. C.2015-08-19T13:49:28Z2015-08-19T13:49:28Z2013FARIAS, Leonardo P.; et al. On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni. PLoS Negl Trop Dis, v.7, n.10, 15p, 2013,https://www.arca.fiocruz.br/handle/icict/1153310.1371/journal.pntd.0002482engPlos OneOn the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil.Tufts University Cummings School of Veterinary Medicine. Department of Infectious Diseases and Global Health. North Grafton, Massachussets, USA.Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil / Universidade de São Paulo. Programa de Pós-Graduação Interunidades em Biotecnologia. São Paulo, SP, Brasil.Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil / Universidade de São Paulo. Programa de Pós-Graduação Interunidades em Biotecnologia. São Paulo, SP, Brasil / Fundação Oswaldo cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Departamento de Imunologia. São Paulo, SP, Brasil.Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil / Universidade de São Paulo. Programa de Pós-Graduação Interunidades em Biotecnologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Departamento de Imunologia. São Paulo, SP, Brasil.Tufts University Cummings School of Veterinary Medicine. Department of Infectious Diseases and Global Health. North Grafton, Massachussets, USA.University of York. Department of Biology. York, UK.Tufts University Cummings School of Veterinary Medicine. Department of Infectious Diseases and Global Health. North Grafton, Massachussets, USA.Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil.Background: It is believed that schistosomes evade complement-mediated killing by expressing regulatory proteins on their surface. Recently, six homologues of human CD59, an important inhibitor of the complement system membrane attack complex, were identified in the schistosome genome. Therefore, it is important to investigate whether these molecules could act as CD59-like complement inhibitors in schistosomes as part of an immune evasion strategy. Methodology/Principal Findings: Herein, we describe the molecular characterization of seven putative SmCD59-like genes and attempt to address the putative biological function of two isoforms. Superimposition analysis of the 3D structure of hCD59 and schistosome sequences revealed that they contain the three-fingered protein domain (TFPD). However, the conserved amino acid residues involved in complement recognition in mammals could not be identified. Real-time RT-PCR and Western blot analysis determined that most of these genes are up-regulated in the transition from free-living cercaria to adult worm stage. Immunolocalization experiments and tegument preparations confirm that at least some of the SmCD59- like proteins are surface-localized; however, significant expression was also detected in internal tissues of adult worms. Finally, the involvement of two SmCD59 proteins in complement inhibition was evaluated by three different approaches: (i) a hemolytic assay using recombinant soluble forms expressed in Pichia pastoris and E. coli; (ii) complement-resistance of CHO cells expressing the respective membrane-anchored proteins; and (iii) the complement killing of schistosomula after gene suppression by RNAi. Our data indicated that these proteins are not involved in the regulation of complement activation. Conclusions: Our results suggest that this group of proteins belongs to the TFPD superfamily. Their expression is associated to intra-host stages, present in the tegument surface, and also in intra-parasite tissues. Three distinct approaches using SmCD59 proteins to inhibit complement strongly suggested that these proteins are not complement inhibitors and their function in schistosomes remains to be determined.Schistosoma mansoniCD59-Like ProteinsThree-Finger Protein Domain FoldSchistosoma mansoniBactériasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11533/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALfloriano_silvajr_etal_IOC-2013.pdfapplication/pdf7298271https://www.arca.fiocruz.br/bitstream/icict/11533/2/floriano_silvajr_etal_IOC-2013.pdf48ea84b3e161930025ae63d506a9698aMD52TEXTfloriano_silvajr_etal_IOC-2013.pdf.txtfloriano_silvajr_etal_IOC-2013.pdf.txtExtracted texttext/plain87507https://www.arca.fiocruz.br/bitstream/icict/11533/3/floriano_silvajr_etal_IOC-2013.pdf.txtde5556c7867f0e6e9f2cd402656781bcMD53icict/115332022-06-24 13:02:59.32oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:02:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
title On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
spellingShingle On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
Farias, Leonardo P.
Schistosoma mansoni
CD59-Like Proteins
Three-Finger Protein Domain Fold
Schistosoma mansoni
Bactérias
title_short On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
title_full On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
title_fullStr On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
title_full_unstemmed On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
title_sort On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni
author Farias, Leonardo P.
author_facet Farias, Leonardo P.
Peterson, Greice Krautz
Tararam, Cibele A.
Montoya, Bogar O. Araujo
Fraga, Tatiana R.
Rofatto, Henrique K.
Silva Jr., Floriano P.
Isaac, Lourdes
Da`dara, Akram A.
Wilson, R. Alan
Shoemaker, Charles B.
Leite, Luciana C. C.
author_role author
author2 Peterson, Greice Krautz
Tararam, Cibele A.
Montoya, Bogar O. Araujo
Fraga, Tatiana R.
Rofatto, Henrique K.
Silva Jr., Floriano P.
Isaac, Lourdes
Da`dara, Akram A.
Wilson, R. Alan
Shoemaker, Charles B.
Leite, Luciana C. C.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Farias, Leonardo P.
Peterson, Greice Krautz
Tararam, Cibele A.
Montoya, Bogar O. Araujo
Fraga, Tatiana R.
Rofatto, Henrique K.
Silva Jr., Floriano P.
Isaac, Lourdes
Da`dara, Akram A.
Wilson, R. Alan
Shoemaker, Charles B.
Leite, Luciana C. C.
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
CD59-Like Proteins
Three-Finger Protein Domain Fold
topic Schistosoma mansoni
CD59-Like Proteins
Three-Finger Protein Domain Fold
Schistosoma mansoni
Bactérias
dc.subject.decs.pt_BR.fl_str_mv Schistosoma mansoni
Bactérias
description Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:28Z
dc.date.available.fl_str_mv 2015-08-19T13:49:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIAS, Leonardo P.; et al. On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni. PLoS Negl Trop Dis, v.7, n.10, 15p, 2013,
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11533
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0002482
identifier_str_mv FARIAS, Leonardo P.; et al. On the Three-Finger Protein Domain Fold and CD59-Like Proteins in Schistosoma mansoni. PLoS Negl Trop Dis, v.7, n.10, 15p, 2013,
10.1371/journal.pntd.0002482
url https://www.arca.fiocruz.br/handle/icict/11533
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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