Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever

Detalhes bibliográficos
Autor(a) principal: Oliveira,KA
Data de Publicação: 2008
Outros Autores: Oliveira,LS, Dias,CCA, Silva Jr,A, Almeida,MR, Almada,G, Bouyer,DH, Galvão,MAM, Mafra,CL
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011
Resumo: Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
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spelling Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted FeverRickettsia felisRickettsia rickettsiiCtenocephalides felisBrazilian Spotted FeverR. felis-spotted fevertickRickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.Instituto Oswaldo Cruz, Ministério da Saúde2008-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011Memórias do Instituto Oswaldo Cruz v.103 n.2 2008reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762008000200011info:eu-repo/semantics/openAccessOliveira,KAOliveira,LSDias,CCASilva Jr,AAlmeida,MRAlmada,GBouyer,DHGalvão,MAMMafra,CLeng2020-04-25T17:50:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:15:31.654Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
title Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
spellingShingle Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
Oliveira,KA
Rickettsia felis
Rickettsia rickettsii
Ctenocephalides felis
Brazilian Spotted Fever
R. felis-spotted fever
tick
title_short Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
title_full Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
title_fullStr Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
title_full_unstemmed Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
title_sort Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever
author Oliveira,KA
author_facet Oliveira,KA
Oliveira,LS
Dias,CCA
Silva Jr,A
Almeida,MR
Almada,G
Bouyer,DH
Galvão,MAM
Mafra,CL
author_role author
author2 Oliveira,LS
Dias,CCA
Silva Jr,A
Almeida,MR
Almada,G
Bouyer,DH
Galvão,MAM
Mafra,CL
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,KA
Oliveira,LS
Dias,CCA
Silva Jr,A
Almeida,MR
Almada,G
Bouyer,DH
Galvão,MAM
Mafra,CL
dc.subject.por.fl_str_mv Rickettsia felis
Rickettsia rickettsii
Ctenocephalides felis
Brazilian Spotted Fever
R. felis-spotted fever
tick
topic Rickettsia felis
Rickettsia rickettsii
Ctenocephalides felis
Brazilian Spotted Fever
R. felis-spotted fever
tick
dc.description.none.fl_txt_mv Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
description Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
publishDate 2008
dc.date.none.fl_str_mv 2008-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000200011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762008000200011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.103 n.2 2008
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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