Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever

Detalhes bibliográficos
Autor(a) principal: Oliveira, KA
Data de Publicação: 2008
Outros Autores: Oliveira, LS, Dias, CCA, Silva Jr, A, Almeida, MR, Almada, G, Bouyer, DH, Galvão, MAM, Mafra, CL
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S0074-02762008000200011
http://www.locus.ufv.br/handle/123456789/12392
Resumo: Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
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spelling Oliveira, KAOliveira, LSDias, CCASilva Jr, AAlmeida, MRAlmada, GBouyer, DHGalvão, MAMMafra, CL2017-10-25T15:06:57Z2017-10-25T15:06:57Z2008-03-201678-8060http://dx.doi.org/10.1590/S0074-02762008000200011http://www.locus.ufv.br/handle/123456789/12392Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.engMemórias do Instituto Oswaldo CruzVol.103, n.2, p.191-194, Mar. 2008Rickettsia felisRickettsia rickettsiiCtenocephalides felisBrazilian spotted feverR. felis-spotted feverTickMolecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted feverinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINAL124.pdf124.pdftexto completoapplication/pdf369568https://locus.ufv.br//bitstream/123456789/12392/1/124.pdf1f1ae8c80d39d4aaa99107f08d3fdf26MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/12392/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAIL124.pdf.jpg124.pdf.jpgIM Thumbnailimage/jpeg4898https://locus.ufv.br//bitstream/123456789/12392/3/124.pdf.jpg74de056263447411dc9bc913ee008413MD53123456789/123922017-10-25 22:00:39.298oai:locus.ufv.br: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Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452017-10-26T01:00:39LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
title Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
spellingShingle Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
Oliveira, KA
Rickettsia felis
Rickettsia rickettsii
Ctenocephalides felis
Brazilian spotted fever
R. felis-spotted fever
Tick
title_short Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
title_full Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
title_fullStr Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
title_full_unstemmed Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
title_sort Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian spotted fever
author Oliveira, KA
author_facet Oliveira, KA
Oliveira, LS
Dias, CCA
Silva Jr, A
Almeida, MR
Almada, G
Bouyer, DH
Galvão, MAM
Mafra, CL
author_role author
author2 Oliveira, LS
Dias, CCA
Silva Jr, A
Almeida, MR
Almada, G
Bouyer, DH
Galvão, MAM
Mafra, CL
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, KA
Oliveira, LS
Dias, CCA
Silva Jr, A
Almeida, MR
Almada, G
Bouyer, DH
Galvão, MAM
Mafra, CL
dc.subject.pt-BR.fl_str_mv Rickettsia felis
Rickettsia rickettsii
Ctenocephalides felis
Brazilian spotted fever
R. felis-spotted fever
Tick
topic Rickettsia felis
Rickettsia rickettsii
Ctenocephalides felis
Brazilian spotted fever
R. felis-spotted fever
Tick
description Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.
publishDate 2008
dc.date.issued.fl_str_mv 2008-03-20
dc.date.accessioned.fl_str_mv 2017-10-25T15:06:57Z
dc.date.available.fl_str_mv 2017-10-25T15:06:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762008000200011
http://www.locus.ufv.br/handle/123456789/12392
dc.identifier.issn.none.fl_str_mv 1678-8060
identifier_str_mv 1678-8060
url http://dx.doi.org/10.1590/S0074-02762008000200011
http://www.locus.ufv.br/handle/123456789/12392
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Vol.103, n.2, p.191-194, Mar. 2008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
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