Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000100006 |
Resumo: | Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 µg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death. |
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Memórias do Instituto Oswaldo Cruz |
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Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venomTrypanosoma cruziprogrammed cell deathBothrops jararacasnake venomCells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 µg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death.Instituto Oswaldo Cruz, Ministério da Saúde2005-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000100006Memórias do Instituto Oswaldo Cruz v.100 n.1 2005reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762005000100006info:eu-repo/semantics/openAccessDeolindo,PolianaTeixeira-Ferreira,André S.Melo,Edésio JTArnholdt,Andrea Cristina VetoSouza,Wanderley deAlves,Elias WDaMatta,Renato Aeng2020-04-25T17:49:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:13:00.006Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
title |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
spellingShingle |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom Deolindo,Poliana Trypanosoma cruzi programmed cell death Bothrops jararaca snake venom |
title_short |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
title_full |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
title_fullStr |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
title_full_unstemmed |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
title_sort |
Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom |
author |
Deolindo,Poliana |
author_facet |
Deolindo,Poliana Teixeira-Ferreira,André S. Melo,Edésio JT Arnholdt,Andrea Cristina Veto Souza,Wanderley de Alves,Elias W DaMatta,Renato A |
author_role |
author |
author2 |
Teixeira-Ferreira,André S. Melo,Edésio JT Arnholdt,Andrea Cristina Veto Souza,Wanderley de Alves,Elias W DaMatta,Renato A |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Deolindo,Poliana Teixeira-Ferreira,André S. Melo,Edésio JT Arnholdt,Andrea Cristina Veto Souza,Wanderley de Alves,Elias W DaMatta,Renato A |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi programmed cell death Bothrops jararaca snake venom |
topic |
Trypanosoma cruzi programmed cell death Bothrops jararaca snake venom |
dc.description.none.fl_txt_mv |
Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 µg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death. |
description |
Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 µg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000100006 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000100006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762005000100006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.100 n.1 2005 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937693141762048 |