Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis

Detalhes bibliográficos
Autor(a) principal: Primon-Barros,Muriel
Data de Publicação: 2015
Outros Autores: Rigo,Graziela Vargas, Frasson,Amanda Piccoli, Santos,Odelta dos, Smiderle,Lisiane, Almeida,Silvana, Macedo,Alexandre José, Tasca,Tiana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000700877
Resumo: Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalisisolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.
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spelling Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalisTrichomonas vaginalisadenosine deaminaseiron chelatorspurinergic systemTrichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalisisolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.Instituto Oswaldo Cruz, Ministério da Saúde2015-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000700877Memórias do Instituto Oswaldo Cruz v.110 n.7 2015reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760150076info:eu-repo/semantics/openAccessPrimon-Barros,MurielRigo,Graziela VargasFrasson,Amanda PiccoliSantos,Odelta dosSmiderle,LisianeAlmeida,SilvanaMacedo,Alexandre JoséTasca,Tianaeng2020-04-25T17:52:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:50.315Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
title Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
spellingShingle Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
Primon-Barros,Muriel
Trichomonas vaginalis
adenosine deaminase
iron chelators
purinergic system
title_short Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
title_full Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
title_fullStr Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
title_full_unstemmed Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
title_sort Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
author Primon-Barros,Muriel
author_facet Primon-Barros,Muriel
Rigo,Graziela Vargas
Frasson,Amanda Piccoli
Santos,Odelta dos
Smiderle,Lisiane
Almeida,Silvana
Macedo,Alexandre José
Tasca,Tiana
author_role author
author2 Rigo,Graziela Vargas
Frasson,Amanda Piccoli
Santos,Odelta dos
Smiderle,Lisiane
Almeida,Silvana
Macedo,Alexandre José
Tasca,Tiana
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Primon-Barros,Muriel
Rigo,Graziela Vargas
Frasson,Amanda Piccoli
Santos,Odelta dos
Smiderle,Lisiane
Almeida,Silvana
Macedo,Alexandre José
Tasca,Tiana
dc.subject.por.fl_str_mv Trichomonas vaginalis
adenosine deaminase
iron chelators
purinergic system
topic Trichomonas vaginalis
adenosine deaminase
iron chelators
purinergic system
dc.description.none.fl_txt_mv Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalisisolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.
description Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalisisolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.
publishDate 2015
dc.date.none.fl_str_mv 2015-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000700877
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000700877
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-02760150076
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.110 n.7 2015
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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