Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/147007 |
Resumo: | Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival. |
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Barros, Muriel Primon deRigo, Graziela de VargasFrasson, Amanda PiccoliSantos, Odelta dosSmiderle, LisianeAlmeida, Silvana Maria deMacedo, Alexandre JoséTasca, Tiana2016-08-10T02:15:45Z20150074-0276http://hdl.handle.net/10183/147007000986547Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.application/pdfengMemórias do Instituto Oswaldo Cruz. Vol. 110, n. 7 (2015), p. 877-883FarmáciaTrichomonas vaginalisAdenosine deaminaseIron chelatorsPurinergic systemModulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalisEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000986547.pdf000986547.pdfTexto completo (inglês)application/pdf563730http://www.lume.ufrgs.br/bitstream/10183/147007/1/000986547.pdfa81dfcdc499ed1547e211769cf4fd416MD51TEXT000986547.pdf.txt000986547.pdf.txtExtracted Texttext/plain35080http://www.lume.ufrgs.br/bitstream/10183/147007/2/000986547.pdf.txt5c5939c6f2b3b98037e65561ada349c7MD52THUMBNAIL000986547.pdf.jpg000986547.pdf.jpgGenerated Thumbnailimage/jpeg2171http://www.lume.ufrgs.br/bitstream/10183/147007/3/000986547.pdf.jpgbd5e8036e3db13529c70527ec0b8c340MD5310183/1470072024-03-20 04:47:51.196933oai:www.lume.ufrgs.br:10183/147007Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-03-20T07:47:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
title |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
spellingShingle |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis Barros, Muriel Primon de Farmácia Trichomonas vaginalis Adenosine deaminase Iron chelators Purinergic system |
title_short |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
title_full |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
title_fullStr |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
title_full_unstemmed |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
title_sort |
Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis |
author |
Barros, Muriel Primon de |
author_facet |
Barros, Muriel Primon de Rigo, Graziela de Vargas Frasson, Amanda Piccoli Santos, Odelta dos Smiderle, Lisiane Almeida, Silvana Maria de Macedo, Alexandre José Tasca, Tiana |
author_role |
author |
author2 |
Rigo, Graziela de Vargas Frasson, Amanda Piccoli Santos, Odelta dos Smiderle, Lisiane Almeida, Silvana Maria de Macedo, Alexandre José Tasca, Tiana |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Barros, Muriel Primon de Rigo, Graziela de Vargas Frasson, Amanda Piccoli Santos, Odelta dos Smiderle, Lisiane Almeida, Silvana Maria de Macedo, Alexandre José Tasca, Tiana |
dc.subject.por.fl_str_mv |
Farmácia |
topic |
Farmácia Trichomonas vaginalis Adenosine deaminase Iron chelators Purinergic system |
dc.subject.eng.fl_str_mv |
Trichomonas vaginalis Adenosine deaminase Iron chelators Purinergic system |
description |
Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-08-10T02:15:45Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10183/147007 |
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0074-0276 |
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000986547 |
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http://hdl.handle.net/10183/147007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Memórias do Instituto Oswaldo Cruz. Vol. 110, n. 7 (2015), p. 877-883 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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