Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population

Detalhes bibliográficos
Autor(a) principal: Velavan,Thirumalaisamy Palanichamy
Data de Publicação: 2011
Outros Autores: Bechlars,Silke, Tomiuk,Jürgen, Kremsner,Peter G, Kun,Jürgen FJ
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100011
Resumo: Parasites remain competent invaders of host immunity. Their invasion strategies have proven to impact immunorelevant genes leading to diversity among gene families. We focussed on signal transducer and activator of transcription (STAT6) factor that plays a fundamental role in signal transduction and activation of transcription. Recent studies have highlighted the role of STAT6 variants in control of infection levels. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the STAT6 gene in a group of Gabonese individuals exposed to a variety of parasitic infections. Three promoter variants were identified in 40 individual subjects. We further validated these promoter variants for their allelic gene expression using transient transfection assays. One promoter variant, rs3024944 (G/C), revealed an altered expression of the marker gene. The identification of function-altering SNPs in the promoter may facilitate studying parasite susceptibility in association studies.
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spelling Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese populationSTAT6polymorphismtransfectionregulatory T cellsParasites remain competent invaders of host immunity. Their invasion strategies have proven to impact immunorelevant genes leading to diversity among gene families. We focussed on signal transducer and activator of transcription (STAT6) factor that plays a fundamental role in signal transduction and activation of transcription. Recent studies have highlighted the role of STAT6 variants in control of infection levels. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the STAT6 gene in a group of Gabonese individuals exposed to a variety of parasitic infections. Three promoter variants were identified in 40 individual subjects. We further validated these promoter variants for their allelic gene expression using transient transfection assays. One promoter variant, rs3024944 (G/C), revealed an altered expression of the marker gene. The identification of function-altering SNPs in the promoter may facilitate studying parasite susceptibility in association studies.Instituto Oswaldo Cruz, Ministério da Saúde2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100011Memórias do Instituto Oswaldo Cruz v.106 n.1 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000100011info:eu-repo/semantics/openAccessVelavan,Thirumalaisamy PalanichamyBechlars,SilkeTomiuk,JürgenKremsner,Peter GKun,Jürgen FJeng2020-04-25T17:50:57Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:27.352Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
title Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
spellingShingle Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
Velavan,Thirumalaisamy Palanichamy
STAT6
polymorphism
transfection
regulatory T cells
title_short Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
title_full Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
title_fullStr Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
title_full_unstemmed Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
title_sort Molecular characterization of regulatory polymorphisms in the promoter region of the STAT6 gene in a Gabonese population
author Velavan,Thirumalaisamy Palanichamy
author_facet Velavan,Thirumalaisamy Palanichamy
Bechlars,Silke
Tomiuk,Jürgen
Kremsner,Peter G
Kun,Jürgen FJ
author_role author
author2 Bechlars,Silke
Tomiuk,Jürgen
Kremsner,Peter G
Kun,Jürgen FJ
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Velavan,Thirumalaisamy Palanichamy
Bechlars,Silke
Tomiuk,Jürgen
Kremsner,Peter G
Kun,Jürgen FJ
dc.subject.por.fl_str_mv STAT6
polymorphism
transfection
regulatory T cells
topic STAT6
polymorphism
transfection
regulatory T cells
dc.description.none.fl_txt_mv Parasites remain competent invaders of host immunity. Their invasion strategies have proven to impact immunorelevant genes leading to diversity among gene families. We focussed on signal transducer and activator of transcription (STAT6) factor that plays a fundamental role in signal transduction and activation of transcription. Recent studies have highlighted the role of STAT6 variants in control of infection levels. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the STAT6 gene in a group of Gabonese individuals exposed to a variety of parasitic infections. Three promoter variants were identified in 40 individual subjects. We further validated these promoter variants for their allelic gene expression using transient transfection assays. One promoter variant, rs3024944 (G/C), revealed an altered expression of the marker gene. The identification of function-altering SNPs in the promoter may facilitate studying parasite susceptibility in association studies.
description Parasites remain competent invaders of host immunity. Their invasion strategies have proven to impact immunorelevant genes leading to diversity among gene families. We focussed on signal transducer and activator of transcription (STAT6) factor that plays a fundamental role in signal transduction and activation of transcription. Recent studies have highlighted the role of STAT6 variants in control of infection levels. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the STAT6 gene in a group of Gabonese individuals exposed to a variety of parasitic infections. Three promoter variants were identified in 40 individual subjects. We further validated these promoter variants for their allelic gene expression using transient transfection assays. One promoter variant, rs3024944 (G/C), revealed an altered expression of the marker gene. The identification of function-altering SNPs in the promoter may facilitate studying parasite susceptibility in association studies.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100011
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000100011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.1 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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