Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener
Autor(a) principal: | |
---|---|
Data de Publicação: | 1997 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600021 |
Resumo: | Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease |
id |
FIOCRUZ-4_893b33d691e92d45cbb54bc392952767 |
---|---|
oai_identifier_str |
oai:scielo:S0074-02761997000600021 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-BrenerTrypanosoma cruzigenome projectphysical mapYac libraryBAC librarysequence tagged sites (STS)expressed sequence tags (EST)Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas diseaseInstituto Oswaldo Cruz, Ministério da Saúde1997-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600021Memórias do Instituto Oswaldo Cruz v.92 n.6 1997reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761997000600021info:eu-repo/semantics/openAccessFerrari,ILorenzi,HSantos,MRBrandariz,SRequena,JMSchijman,AVázquez,MSilveira,JF daBen-Dov,CMedrano,CGhío,SLópez Bergami,PCano,IZingales,BUrmenyi,TPRondinelli,EGonzález,ACortes,ALopez,MCThomas,MCAlonso,CRamírez,JLChiurrillo,MAAldao,R RangelBrandão,ADegrave,WPerrot,VSaumier,MBillaut,ACohen,DLe Paslier,DLevin,MJeng2020-04-25T17:47:46Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:44.56Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
title |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
spellingShingle |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener Ferrari,I Trypanosoma cruzi genome project physical map Yac library BAC library sequence tagged sites (STS) expressed sequence tags (EST) |
title_short |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
title_full |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
title_fullStr |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
title_full_unstemmed |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
title_sort |
Towards the Physical Map of the Trypanosoma cruzi Nuclear Genome: Construction of YAC and BAC Libraries of the Reference Clone T. cruzi CL-Brener |
author |
Ferrari,I |
author_facet |
Ferrari,I Lorenzi,H Santos,MR Brandariz,S Requena,JM Schijman,A Vázquez,M Silveira,JF da Ben-Dov,C Medrano,C Ghío,S López Bergami,P Cano,I Zingales,B Urmenyi,TP Rondinelli,E González,A Cortes,A Lopez,MC Thomas,MC Alonso,C Ramírez,JL Chiurrillo,MA Aldao,R Rangel Brandão,A Degrave,W Perrot,V Saumier,M Billaut,A Cohen,D Le Paslier,D Levin,MJ |
author_role |
author |
author2 |
Lorenzi,H Santos,MR Brandariz,S Requena,JM Schijman,A Vázquez,M Silveira,JF da Ben-Dov,C Medrano,C Ghío,S López Bergami,P Cano,I Zingales,B Urmenyi,TP Rondinelli,E González,A Cortes,A Lopez,MC Thomas,MC Alonso,C Ramírez,JL Chiurrillo,MA Aldao,R Rangel Brandão,A Degrave,W Perrot,V Saumier,M Billaut,A Cohen,D Le Paslier,D Levin,MJ |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferrari,I Lorenzi,H Santos,MR Brandariz,S Requena,JM Schijman,A Vázquez,M Silveira,JF da Ben-Dov,C Medrano,C Ghío,S López Bergami,P Cano,I Zingales,B Urmenyi,TP Rondinelli,E González,A Cortes,A Lopez,MC Thomas,MC Alonso,C Ramírez,JL Chiurrillo,MA Aldao,R Rangel Brandão,A Degrave,W Perrot,V Saumier,M Billaut,A Cohen,D Le Paslier,D Levin,MJ |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi genome project physical map Yac library BAC library sequence tagged sites (STS) expressed sequence tags (EST) |
topic |
Trypanosoma cruzi genome project physical map Yac library BAC library sequence tagged sites (STS) expressed sequence tags (EST) |
dc.description.none.fl_txt_mv |
Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease |
description |
Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600021 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761997000600021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.92 n.6 1997 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937671574650880 |