Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes

Detalhes bibliográficos
Autor(a) principal: Saldarriaga,Omar A
Data de Publicação: 2003
Outros Autores: Velásquez,Jaime I, Ossa,Jorge E, Rugeles,Maria T
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017
Resumo: We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
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spelling Standardization of bovine macrophage monolayers and isolation and culture of trypanosomesmacrophagesbovinetrypanosomesWe describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.Instituto Oswaldo Cruz, Ministério da Saúde2003-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017Memórias do Instituto Oswaldo Cruz v.98 n.2 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000200017info:eu-repo/semantics/openAccessSaldarriaga,Omar AVelásquez,Jaime IOssa,Jorge ERugeles,Maria Teng2020-04-25T17:49:01Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:46.784Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
spellingShingle Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
Saldarriaga,Omar A
macrophages
bovine
trypanosomes
title_short Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_full Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_fullStr Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_full_unstemmed Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
title_sort Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
author Saldarriaga,Omar A
author_facet Saldarriaga,Omar A
Velásquez,Jaime I
Ossa,Jorge E
Rugeles,Maria T
author_role author
author2 Velásquez,Jaime I
Ossa,Jorge E
Rugeles,Maria T
author2_role author
author
author
dc.contributor.author.fl_str_mv Saldarriaga,Omar A
Velásquez,Jaime I
Ossa,Jorge E
Rugeles,Maria T
dc.subject.por.fl_str_mv macrophages
bovine
trypanosomes
topic macrophages
bovine
trypanosomes
dc.description.none.fl_txt_mv We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
description We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.
publishDate 2003
dc.date.none.fl_str_mv 2003-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762003000200017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.98 n.2 2003
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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